# Profoundd archive — Epstein Files
# Bates number: EFTA01032604
# Title:        From: Joichi Ito
# Dataset:      9
# Pages:        2
# Tags:         epstein, doj, dataset-9
# Source PDF:   https://profoundd.com/epstein-docs/EFTA01032604/download
# Doc viewer:   https://profoundd.com/epstein-docs/EFTA01032604
#
# Text below is what Profoundd has extracted from the source PDF.
# 'ocr-enriched' tag means OCR was applied to scan-only pages.
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=== SUMMARY ===
From: Joichi Ito To: Jeffrey Epstein <jeevacation@gmail.com> Subject: Fwd: Mass Spec device Date: Thu, 07 Sep 2017 20:53:11 +0000 This machine will kick off the collaboration between Caleb and Ed to work on the plant/animal stuff. Begin forwarded message: From: Arielle Johnson Subject: Re: Mass Spec device Date: September 5, 2017 at 3:07:36 PM EDT To: Caleb Harper Cc: Edward Boyden , Joichi Ito <ti Hi-> We are getting a Gas Chromatograph-Mass Spectrometer System from Agilent Technologies with a


=== EXTRACTED TEXT ===
From: Joichi Ito
To: Jeffrey Epstein <jeevacation@gmail.com> 
Subject: Fwd: Mass Spec device 
Date: Thu, 07 Sep 2017 20:53:11 +0000 
This machine will kick off the collaboration between Caleb and Ed to work on the plant/animal stuff. 
Begin forwarded message: 
From: Arielle Johnson 
Subject: Re: Mass Spec device 
Date: September 5, 2017 at 3:07:36 PM EDT 
To: Caleb Harper 
Cc: Edward Boyden , Joichi Ito <ti 
Hi-> 
We are getting a Gas Chromatograph-Mass Spectrometer System from Agilent Technologies with a 7890B 
model GC and a 5975 triple qudrupole electron impact mass spec. 
The autosampler is made by a different company called Gerstel. It is a Gerstel MPS model autosampler. 
The whole setup works together to accurately extract, sample, separate, and identify/quantify mixtures of 
volatile (i.e. in equilibrium conditions, spending a significant amount of time in the gas phase) molecules. 
To detect and analyze these molecules, which tend to be in sub-part per million concentrations and inside plant 
tissue and other solids/liquids, a sampling and concentration step is necessary. Previously (and some labs still 
do this) a solvent extraction -distillation process was used to do this, but this is time consuming, labor-intensive, 
uses hazardous materials, and is often not satisfactorily representative of the proportions of molecules that are 
smelled-i.e., in the gas phase. Around 20 years ago or so an analytical chemistry group in the Czech republic 
developed a technique that samples from the headspace above a sample (recall that since we are measuring 
volatiles, the molecules of interest spend some of their time in the gas phase) and uses and absorptive polymer 
instead of a liquid solvent. 
This technique (headspace-solid phase microextraction or SPME) has the added benefit of being highly 
automatable—instead of having a graduate student do a series of timed extractions on the bench with a 
stopwatch, it became possible to aliquot out several sample into vials, and perform a timed/temperature 
controlled sampling and analysis of each with an autosampler robot. It's still necessary to prep the samples by 
hand and there's a lot of hands-on parts to the data analysis. but it greatly increases throughput and accuracy. 
This is the GC model we're getting, refurbished: 
chromatography/gc-systems/7890b-gc
and this is a brochure for the GC-MS setup hup://hpst.cz/sites/default/files/attachments/5989-7827en-5975-
broschure-Ir.pdf
Agilent makes an autosampler, but for our extraction technique I've always used, and have always had 
recommended to use, the Gerstel model. 
this is the autosampler: 
this is some info from Gerstel about SPME: 
EFTA01032604
let me know if I can clarify anything; I'll be in Cambridge in person next week 
best 
Arielle 
EFTA01032605