01 MMRV safety summary 508

CDC ACIP — Vaccine Advisory Committee

Acip

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MMRV Vaccine Safety  
 
Contents  
Summary  ................................ ................................ ................................ ................................ ......  1 
Cochrane Review  ................................ ................................ ................................ ...........................  2 
General safety  ................................ ................................ ................................ ...............................  3 
Febrile convulsion/febrile seizure  ................................ ................................ ................................ .... 6 
Studies performed outside of the United States  ................................ ................................ ................  8 
Clinical Trial data  ................................ ................................ ................................ .........................  10 
 
Summary  
The measles -mumps -rubella -varicella (MMRV) vaccine (ProQuad®, Merck) was licensed in the 
United States in 2005 for use in children 12 months through 12 years of age. In 2006, the 
Advisory Committee on Immunization Practices (ACIP) recommended MMRV as an option for 
both the first and second doses of measles, mumps, rubella, and varicella vaccination. However, 
post -licensure safety surveillance identified an increased risk of febrile seizures 5 –12 days aft er 
the first dose in children aged 12 –23 months compared to those receiving separate MMR and 
varicella (MMR + V) vaccines. Based on this finding, ACIP updated its guidance in 2009.  
ACIP currently recommends that MMR  and varicella vaccines be given separately for the first 
dose in children 12 –47 months ; however , MMRV may be  used if parents or caregivers request a 
preference. Compared with use of MMR vaccine and varicella vaccine at the same visit, use of 
MMRV vaccine results in one fewer injection but is associated with a higher risk for fever and 
febrile seizures 5 -12 days after the first dose among children aged 12 -23 months . Use of MMR 
vaccine and varicella vaccine avoids this increased risk for fever and febrile seizures following 
MMRV vaccine.  Studies of febrile seizures after vaccination with first dose of MMRV vaccine 
have not been done in older children, but experts agree that this increased risk of fever and 
febrile seizures during the 5 -12 days after first dose vaccination likely also occur s in ch ildren 
aged 24 -47 months because that is the biologic window of vulnerability for febrile seizures in 
children (approximately 97% of febrile seizures occur in children aged <4 years). First febrile 
seizures are uncommon after age 4 years  (MMRV Questions and Answers for Healthcare 
Providers | CDC ). Either MMRV or MMR + V may be used for the second dose or for children 
aged 4 years and older  (Use of Combination Measles, Mumps, Rubella, and Varicella Vaccine ). 
Over the past 15+ years  and multiple studies , mild fever and rash are the most common ly 
reported adverse events  following MMRV vaccination . The primary safety concern is an 
increased risk of febrile seizures following the first dose in children aged 12 -23 months.  This risk 
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is estimated at 1 additional seizure occurring 5 -12 days after vaccination per 2,300 -2,600 
vaccinated children  compared to those receiving MMR + V separately  (Use of Combination 
Measles, Mumps, Rubella, and Varicella Vaccine ).  These events resolve without long -term 
consequences  (Measles, Mumps, Rubella, Varicella (MMRV) Vaccine Safety | Vaccine Safety | 
CDC). No increased seizure risk has been observed with the second dose or in children aged 48 
months or older.  
Several clinical trials have also evaluated the safety of MMRV when administered concomitantly 
with other routine childhood vaccines , including pneumococcal conjugate vaccine (PCV -7), 
meningococcal conjugate vaccines (MenACWY , MenC), and DTaP -containing vaccines. These 
studies found no increase in serious adverse events or clinically meaningful differences in 
reactogenicity .  International surveillance and real-world  data from Canada and Europe have  
confirm ed the most common adverse events as mild fever and rash , along with the increased 
risk of febrile seizure as the only serious adverse event  following MMRV vaccination. Although 
rare case s of encephalitis and death have been reported after MMRV vaccination, no direct link 
between these events and the vaccine has been established in persons with healthy immune 
systems . 
Cochrane Review  
• Di Pietrantonj C, Rivetti A, Marchione P , Debalini MG, Demicheli V. Vaccines for measles, 
mumps, rubella, and varicella in children.  Cochrane Database Syst Rev . 
2021;11(11):CD004407. Published 2021 Nov 22. doi:10.1002/14651858.CD004407.pub5  
o Authors searched the Cochrane Central Register of Controlled Trials (CENTRAL) (the 
Cochrane Library 2019, Issue 5), which includes the Cochrane Acute Respiratory 
Infections Group's Specialised Register, MEDLINE (1966 to 2 May 2019), Embase 
(1974 to 2 May 2019), th e WHO International Clinical Trials Registry Platform (2 May 
2019), and ClinicalTrials.gov (2 May 2019).  
o Includes randomized  controlled trials (RCTs), controlled clinical trials (CCTs), 
prospective and retrospective cohort studies (PCS/RCS), case-control studies (CCS), 
interrupted time-series (ITS) studies, case cross-over (CCO) studies, case-only 
ecological method (COEM) studi es, self-controlled case series (SCCS) studies, person-
time cohort (PTC) studies, and case-coverage design/screening methods (CCD/SM) 
studies, assessing any combined MMR or MMRV / MMR+V vaccine given in any 
dose, preparation or time sche dule compared with no intervention or placebo, on 
healthy children up to 15 years of age.  
o Seizure:  The analyses provide evidence supporting an association between 
MMR/MMR+V/MMRV vaccines (Jeryl Lynn strain) and febrile seizures. Febrile 
seizures normally occur in 2% to 4% of healthy children at least once before the 
age of 5. The attributable risk febri le seizures vaccine‐induced is estimated to be 
from 1 per 1700 to 1 per 1150 administered doses.  
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o ITP: ITP can happen after natural measles infection.  The analyses provide evidence 
supporting an association between MMR  vaccination and ITP .  However, the risk of 
ITP after vaccination is smaller than after natural infection with these viruses. Natural 
infection of ITP occur in 5 cases per 100,000 (1 case per 20,000) per year. The 
attributable risk is estimated about 1 case of ITP per 40,000 adm inistered MMR 
doses.
 The overall meta-analysis estimate of association between MMR 
vaccination and ITP in children aged 9 to 23 months was RR 4.21 (95% CI 2.28 to 
7.78). There was no statistical evidence in children aged 4 to 6 y ears ( RR 3.06, 95% CI 
0.42 to 22.30), and no statistical evidence of association between MMRV 
vaccination and ITP in children aged 9 to 23 months ( RR 2.87, 95% CI 0.78 to 10.56).  
The latter two results came from one study ( db-O'Leary 2012 ). 
General safety  
• Klopfer SO, Stek JE, Petrecz M, et al. Analysis of safety data in children after receiving two 
doses of ProQuad® (MMRV).  Vaccine . 2014;32(52):7154 -7160. 
doi:10.1016/j.vaccine.2014.08.067  
o Safety data from five clinical studies were combined for all children who were 
scheduled to receive two doses of MMRV ∼3-6 months apart. All vaccinated children 
were followed for safety following each dose of MMRV.  
o Of 3112 children who received a first dose of MMRV, 2780 (89.3%) received a second 
dose of MMRV. Overall, 70.5% and 57.7% of children reported ≥1 adverse 
experiences following first and second doses of MMRV, respectively . Injection ‐site 
redness  was statistically significantly higher postdose 2 than postdose 1, while 
injection ‐site pain/tenderness  was statistically significantly higher postdose 1 
compared to postdose 2. Rashes  were statistically significantly lower postdose 2 
compared to postdose 1. Ten febr ile seizures  (8 postdose 1, 2 postdose 2) were 
reported following MMRV vaccination. The incidence of febrile seizures postdose 1 
of MMRV was 0.26% (8/3019) compared to 0.07% (2/2695) postdose 2 of MMRV.  
o Author’s conclusions: a dministration of two doses of MMRV has an acceptable safety 
profile in children 12 to 23 months of age. There is a small increase in the risk of 
febrile seizures following the first dose of MMRV as compared to the component 
vaccines, but the risk for any i ndividual child is relatively low.  
• Klein NP , Lewis E, Fireman B, et al. Safety of measles -containing vaccines in 1 -year -old 
children.  Pediatrics . 2015;135(2):e321 -e329. doi:10.1542/peds.2014 -1822  
o Study children were aged 12 to 23 months in the Vaccine Safety Datalink from 2000 
to 2012. Nine study outcomes were investigated : 7 main outcomes (anaphylaxis, 
ITP, ataxia, arthritis, meningitis/encephalitis, acute disseminated 
encephalomyelitis, and Kawasaki disease), seizure, and fever.  Comparing MMRV 
with MMR + V, relative risk was estimated by using stratified exact binomial tests. 
Secondary analyses examined post -MMRV or MMR + V risk versus comparison 
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intervals; risk and comparison intervals were then contrasted for MMRV versus 
MMR+V.  
o Authors  evaluated 123,200 MMRV and 584,987 MMR + V doses . Comparing MMRV 
with MMR + V, risks for the 7 main outcomes were not significantly different.  
Several outcomes had few or zero postvaccination events. Comparing risk versus 
comparison intervals, ITP risk was higher after MMRV (odds ratio [OR]: 11.3 [95% 
confidence interval (CI): 1.9 to 68.2]) and MMR + V (OR: 10 [95% CI: 4.5 to 22.5]) 
and ataxia risk was lower after both vaccines (MMRV OR: 0.8 [95% CI: 0 .5 to 1]; 
MMR + V OR: 0.8 [95% CI: 0.7 to 0.9]).  Compared with MMR + V, MMRV increased 
risk of seizure and fever 7 to 10 days after vaccination.  
o Author’s conclusions: This study did not identify any new safety concerns comparing 
MMRV with MMR + V or after either the MMRV or the MMR + V vaccine. This study 
provides reassurance that these outcomes are unlikely after either vaccine.  
• Ma SJ, Li X, Xiong YQ, Yao AL, Chen Q. Combination Measles -Mumps -Rubella -Varicella 
Vaccine in Healthy Children: A Systematic Review and Meta -analysis of Immunogenicity and 
Safety.  Medicine (Baltimore) . 2015;94(44):e1721. doi:10.1097/MD.0000000000001721  
o Authors  searched PubMed, Embase, BIOSIS Previews, Web of Science, Cochrane 
Library, and other databases through September 9, 2014. Eligible randomized 
controlled trials (RCTs) were selected and collected independently by 2 reviewers. 
Meta -analysis was conducted u sing Stata 12.0 and RevMan 5.3.  
o Incidences of any serious adverse events (SAEs) were around 1% in all the groups; 
only about one -tenth of the events were considered to be related to vaccination 
studied. About half of the related SAEs were febrile seizures. The incidence of related 
febril e seizure was under 0.8 % in MMRV groups and under 0.5‰ in MMR + V/MMR 
groups. No statistical difference was found between groups with no evidence of 
heterogeneity . No related fatal SAE was reported in any studies included.  
o Well tolerated safety profiles were demonstrated except higher incidence of fever 
(relative risks 1.12 –1.60) and measles/rubella ‐like rash (relative risks 1.44 –1.45) in 
MMRV groups.  
o Author’s conclusions: MMRV had comparable immunogenicity and overall safety 
profiles to MMR + V/MMR in healthy children based on current evidence.  
• Woo EJ, Winiecki SK, Arya D, Beeler J. Adverse Events After MMR or MMRV Vaccine in 
Infants Under Nine Months Old.  Pediatr Infect Dis J . 2016;35(8):e253 -e257. 
doi:10.1097/INF.0000000000001201  
o The Vaccine Adverse Event Reporting System  was searched  for reports of measles, 
mumps and rubella vaccine (MMR) or measles, mumps, rubella and varicella vaccine 
(MMRV) vaccination in children less than 9 months of age. A clinical assessment of 
each report was conducted and the frequency, range, onset time and severity of 
adverse events  was summarized . 
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o After excluding 346 reports because they were duplicates or because they contained 
insufficient information about the child's age or vaccine(s), authors  retained 204 
reports in the analysis, including 35 (17%) that were serious. Among the 169 
nonserious reports, more than half (88; 52%) described a vaccination error without 
any adverse event per se. Other nonserious reports described fever, injection 
reactions and gastrointestinal symptoms . Serious adverse events included 
developmental disorders, fever and fussiness. There were 44 reports of fever, but 
only 4 cases began 5 ‐12 days after immunization, the peak risk window. The vast 
majority of fever reports listed concomitant vaccines, such as diphtheria and 
tetanus toxoids, acellular or whole ‐cell pertussis vaccine.  
o Author’s conclusions:  This review did not identify any major safety concerns. These 
findings may facilitate discussions about the risks and benefits of vaccinating infants 
who are potentially exposed to this life -threatening disease.  
• Safety Surveillance of Varicella Vaccines in the Vaccine Adverse Event Reporting System, United 
States, 2006 –2020 - PMC  
o US VAERS reports received after administration of VAR and MMRV during 2006 -2020 
were identified. Reports were analyzed by vaccine type, age, seriousness, most 
common adverse events (AEs), and concomitant vaccines. Medical records of 
selected reports of AEs of special interest were reviewed and empirical Bayesian data 
mining to identify disproportionally reported AEs  was conducted . 
o During 2006 -2020, approximately 132.8 million VAR doses were distributed; 40 684 
reports were received in VAERS (30.6/100 000 doses distributed), with 4.1% 
classified as serious (1.3/100 000 doses distributed). Approximately 35.5 million 
MMRV doses were di stributed; 13 325 reports were received (37.6/100 000 doses 
distributed) with 3.3% classified as serious (1.3/100 000 doses distributed). The most 
common adverse health events after both VAR and MMRV were injection site 
reactions (31% and 27%), rash (28% a nd 20%), and fever (12% and 14%), 
respectively. Vaccination errors accounted for 23% of reports after VAR 
administration and 41% after MMRV administration, but ≥95% of them did not 
describe an adverse health event. AEs associated with evidence of vaccine s train 
varicella -zoster virus (vVZV) infection included meningitis, encephalitis, herpes 
zoster, and 6 deaths (all in immunocompromised persons with contraindications for 
vaccination). No new or unexpected AE was disproportionally reported . 
o Author’s conclusions: No new or unexpected safety findings were detected for VAR 
and MMRV given as recommended, reinforcing the favorable safety profiles of these 
vaccines. Providers should obtain specimens for viral testing and strain -typing for 
serious AEs if they consider vV ZV as the possible causative agent.  
• Vittrup DM, Charabi S, Jensen A, Stensballe LG. A systematic review and meta -analysis of 
adverse events following measles -containing vaccines in infants less than 12 months of 
age. Vaccine . 2025;47:126687. doi:10.1016/j.vaccine.2024.126687  
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o EMBASE and PubMed were searched in February 2021 , and the search was  updated 
in February 2024. With the exception of case reports, we included all English -written 
original studies published >1985 that contained frequency measures on adverse 
events (AEs) within 56  days following MCV1 in infants <12  months of age. We 
identified all common AEs and their frequencies and combined these across studies 
in a meta -analysis. The effect of measles strain and vaccine valency  was also 
evaluated.  
o 24 studies were included in the analysis: 18 randomized controlled trials (RCTs), 
three interventional studies, and three observational studies. Only one RCT was 
placebo -controlled. Commonly reported AEs were injection site reactions, fever, 
rash, gastrointestinal symptoms, respiratory t ract symptoms, conjunctivitis, and 
symptoms related to the general condition of the infant . The frequency of any AE 
was generally <10  %; however, the placebo ‐controlled trial showed no difference 
between MCV1 and placebo ‐injected infants. Edmonston B strains and measles ‐
mumps ‐rubella ‐varicella vaccine (MMRV) were associated with a higher rate of 
high fever >39  °C. 
o Most AEs occurred in <10  % of infants receiving MCV1  at < 12 months of age. The 
placebo -controlled trial suggested no excess reactogenicity following early MCV. 
Measles strain and vaccine valency may affect AE risks, but other factors such as 
socioeconomic  status, race, and setting could also explain this finding, as these were 
not equally distributed between studies. Caution is advised when interpreting 
findings from studies without a placebo group.  
Febrile convulsion/febrile seizure  
• Jacobsen SJ, Ackerson BK, Sy LS, et al. Observational safety study of febrile convulsion 
following first dose MMRV vaccination in a managed care setting.  Vaccine . 
2009;27(34):4656 -4661. doi:10.1016/j.vaccine.2009.05.056  
o Children ages 12 –60 months who received a first dose of MMRV in February 
2006 –June 2007 in a managed care organization were included in the study. 
Subjects were optimally matched on age, sex, and calendar date of vaccination to 
children who received MMR  + V concomitantly in November 2003 –January 2006, 
before MMRV licensure. Potential cases of febrile convulsion were identified 
through administrative data and adjudicated by expert panel, according to pre -
specified criteria.  
o During the 30 days post -vaccination, there were 128 and 94 potential convulsion 
cases among the 31,298 children in the MMRV and MMR+V cohorts, 
respectively. After review of available medical charts and adjudication, there 
were 84 cases of confirmed febrile  convulsion, 44 (1.41/1000) and 40 
(1.28/1000) in the MMRV and MMR+V cohorts, respectively (RR=1.10, 95% 
CI=0.72, 1.69). In days 5 -12 following vaccination, a pre -specified period of 
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interest, the respective numbers were 22 (0.70/1000) and 10 (0.32/1000) 
(RR=2.20, 95% CI=1.04, 4.65).  
o These data suggest that the risk of febrile convulsion is increased in days 5 ‐12 
following vaccination with MMRV as compared to MMR+V given separately 
during the same visit, when post ‐vaccination fever and rash are also increased 
in clinical trials.   
• Klein NP , Fireman B, Yih WK, et al. Measles -mumps -rubella -varicella combination vaccine 
and the risk of febrile seizures.  Pediatrics . 2010;126(1):e1 -e8. doi:10.1542/peds.2010 -
0665  
o Using 2000 –2008 Vaccine Safety Datalink data, authors assessed seizures and 
fever visits among children aged 12 to 23 months after MMRV and separate 
MMR + varicella vaccines. Authors  compared seizure risk after MMRV vaccine to 
that after MMR + varicella vaccines by using Poisson regression as well as with 
supplementary regressions that incorporated chart -review results and self -
controlled analyses.  
o MMRV vaccine recipients (83 107) were compared with recipients of MMR + 
varicella vaccines (376 354). Seizure and fever significantly clustered 7 to 10 days 
after vaccination with all measles -containing vaccines but not after varicella 
vaccination alone. S eizure risk during days 7 to 10 was higher after MMRV than 
after MMR + varicella vaccination (relative risk: 1.98 [95% confidence interval: 
1.43 –2.73]). Supplementary analyses yielded similar results. The excess risk for 
febrile seizures 7 to 10 days after  MMRV compared with separate MMR + 
varicella vaccination was 4.3 per 10 000 doses (95% confidence interval: 2.6 –5.6).  
o Among 12 ‐ to 23 ‐month ‐olds who received their first dose of measles ‐
containing vaccine, fever and seizure were elevated 7 to 10 days after 
vaccination. Vaccination with MMRV results in 1 additional febrile seizure for 
every 2300 doses given instead of sepa rate MMR + varicella vaccines.  
• Klein NP , Lewis E, Baxter R, et al. Measles -containing vaccines and febrile seizures in 
children age 4 to 6 years.  Pediatrics . 2012;129(5):809 -814. doi:10.1542/peds.2011 -3198  
o Among 4 - to 6-year -old Vaccine Safety Datalink members, authors  identified 
seizures in the emergency department and hospital from 2000 to 2008 and 
outpatient visits for fever from 2006 to 2008 during days 7 to 10 and 0 to 42 after 
MMRV and MMR + V. Incorporating medical record reviews, we assessed seizure 
risk after M MRV and MMR + V.  
o From 2006 through 2008, 86 750 children received MMRV; from 2000 through 
2008, 67 438 received same -day MMR + V. Seizures were rare throughout days 0 
to 42 without peaking during days 7 to 10. There was 1 febrile seizure 7 to 10 
days after MMRV and 0 after  MMR + V. Febrile seizure risk was 1 per 86 750 
MMRV doses (95% confidence interval, 1 per 3 426 441, 1 per 15 570) and 0 per 
67 438 MMR + V doses (1 per 18 282).  
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o This study provides reassurance that MMRV and MMR + V were not associated 
with increased risk of febrile seizures among 4 ‐ to 6‐year ‐olds. We can rule out 
with 95% confidence a risk greater than 1 febrile seizure per 15 500 MMRV 
doses and 1 per 18 000 MMR + V doses.  
• Ma SJ, Xiong YQ, Jiang LN, Chen Q. Risk of febrile seizure after measles -mumps -rubella -
varicella vaccine: A systematic review and meta -analysis.  Vaccine . 2015;33(31):3636 -
3649. doi:10.1016/j.vaccine.2015.06.009  
o PubMed, Embase, BIOSIS Previews, Scopus, Web of Science, Cochrane Library 
and other databases  were searched  through 12 December 2014.  
o A total of thirty -nine studies were included. Thirty -one published or unpublished 
clinical trials involving about 40,000 subjects did not show significant differences 
in incidence of febrile seizure or vaccine related febrile seizure between MMRV 
and MMR w ith or without varicella vaccine after any doses, in the risk windows 
of 0-28, 0 -42 or 0 -56 days and 7 -10 days. In addition, these studies showed that 
the receipt of concomitant use of MMRV and other pediatric vaccines was not a 
significant predictor of fe brile seizure. Eight post -marketing observations 
involving more than 3,200,000 subjects were included. No evidence suggested 
elevated risk of febrile seizure associated with MMRV vaccine among children 
aged 4 ‐6 years old during 7 ‐10 days or 0 ‐42 days after vaccination. However, an 
approximately 2 ‐fold increase in risk of seizure or febrile seizure during 7 ‐10 
days or 5 ‐12 days after MMRV vaccination was found among children aged 10 ‐
24 months, although the highest incidence of seizure was still lower than 
2.95%. 
o First MMRV vaccine dose in children aged 10 -24 months was associated with an 
elevated risk of seizure or febrile seizure.  
Studies performed outside of the United States  
Canada  
• Seo CY , Rashid M, Harris T, Stapleton J, Deeks SL. Assessing safety of Ontario's publicly 
funded MMR and MMRV immunization programs, 2012 to 2016.  Paediatr Child Health . 
2019;25(6):358 -364. Published 2019 Apr 8. doi:10.1093/pch/pxz037  
o Reports of AEFIs were extracted from the provincial surveillance database on 
May 9, 2017. Events were grouped by provincial surveillance definitions. 
Reporting rates were calculated using provincial population estimates or net 
doses distributed as the deno minator. A serious AEFI is defined as an AEFI that 
resulted in an in -patient hospitalization or death.  
o  Overall, 289 AEFIs were reported following administration of MMR (n=246) or 
MMRV (n=43) vaccines, for annualized reporting rates of 16.6 and 8.8 reports per 
100,000 distributed doses, respectively. The highest age -specific reporting rate 
was in children ag ed 1 to 3 years for MMR (7.7 per 100,000 population) and 
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children aged 4 to 9 years for MMRV (0.8 per 100,000 population). Systemic 
reactions were the most frequently reported event category, while rash was the 
most frequently reported event for both vaccines. There were 22 serious AEFIs, 
19 following MMR and 3 following MMRV (1.3 and 0.6 per 100,000 doses 
distributed, respectively).  
o Authors  found a low reporting rate of adverse events following MMR and MMRV 
vaccines in Ontario. No safety concerns were identified.  
Germany  
• Schäfer W, Reinders T, Schink T. Second dose of measles -mumps -rubella -varicella vaccine 
(MMRV) and the risk of febrile convulsions.  Vaccine . 2022;40(14):2168 -2172. 
doi:10.1016/j.vaccine.2022.02.072  
o A retrospective cohort study using claims data from the German 
Pharmacoepidemiological Research database (GePaRD) was performed in 
children born between January 1st, 2004 and October 31st, 2015 who received 
two doses of MMRV, MMR  + V or MMR. Cases were defi ned as hospitalization 
with a diagnosis of febrile convulsions ( FC) without neurological conditions coded 
as main discharge diagnosis. Unadjusted and adjusted odds ratios (OR) with 95% 
confidence inte rvals (CIs) were calculated to compare the risk of FC. Stratified 
analyses were performed to examine potential effect modification by age, sex, 
history of FC or type of first dose vaccine.  
o In the first 30  days after second dose  vaccination , 464 FCs were observed in a 
cohort of 528,639 children with a median age of 17  months. After adjustment for 
potential confounders, the adjusted OR for FC in the 30  days after vaccination 
was 1.25 (95% CI 0.67 –2.30) for MMRV compared to MMR  + V and 1.04 (0 .82–
1.32) for MMRV compared to MMR. History of FC was the most important risk 
factor with an OR of 36.26 (29.30 –44.89). We found no effect modification by 
age, sex, history of FC, or type of first dose vaccine.  
o Author’s conclusions: Use of MMRV at second dose is not associated with an 
increased risk of FC compared to MMR  + V or MMR, irrespective of age, sex, 
history of FC, or type of first dose vaccine.  
Italy  
• Stefanizzi P , Stella P , Ancona D, et al. Adverse Events Following Measles -Mumps -Rubella -
Varicella Vaccination and the Case of Seizures: A Post Marketing Active Surveillance in 
Puglia Italian Region, 2017 -2018.  Vaccines (Basel) . 2019;7(4):140. Published 2019 Oct 7. 
doi:10.3390/vaccines7040140  
o In the Puglia Region launched, from May 2017 to November 2018, a post -
marketing active surveillance program of adverse events following MMRV 
immunization (AEFIs). Immunized children (second year of life) were enrolled on 
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a voluntary basis, AEFIs diaries were used, and their parents were interviewed 25 
days after the immunization.  
o There were 2540 children enrolled; 2149/2540 (84.6%) completed the post -
vaccination follow -up. Of these, 992 AEFIs were registered with a reporting rate 
of 46.2 × 100 doses: 883/992 (89.0%) AEFIs were not serious, while 109/992 
(11.0%) were serious. For se rious AEFIs, the evaluation of causality assessment 
was performed using the algorithm proposed by the World Health Organisation 
(WHO): 82/109 consistent causal associations to MMRV immunization were 
detected (reporting rate of consistent AEFIs: 3.8 × 100 f ollow -up). All serious 
AEFIs consistently associated with immunization resulted completely resolved at 
the follow -up. The reporting rate of seizure consistently associated with 
immunization was 0.05 × 100, lower than data previous published in the 
literatu re that did not report the causality assessment.  
o Author’s conclusion: Because  no emerging signals were detected, data from the 
active surveillance program confirmed the safety profile of the MMRV vaccine.  
Clinical Trial data  
• Reisinger KS, Brown ML, Xu J, et al. A combination measles, mumps, rubella, and varicella 
vaccine (ProQuad) given to 4 - to 6-year-old healthy children vaccinated previously with M -
M-RII and Varivax [published correction appears in Pediatrics. 2006 
Jun;117(6):2338].  Pediatrics . 2006;117(2):265 -272. doi:10.1542/peds.2005 -0092  
o Four- to 6-year-old children who had been immunized previously with M -M-RII and 
Varivax were assigned randomly to receive either ProQuad and placebo ( N = 399), M -
M-RII and placebo ( N = 195), or M -M-RII and Varivax ( N = 205) and were then 
monitored for safety and immunogenicity.  ProQuad was generally well tolerated.  
Similarity (noninferiority) was demonstrated in postvaccination antibody responses 
to measles, mumps, and rubella between recipients of ProQuad and all recipients 
of M-M-RII and in responses to varicella between recipients of ProQuad and 
recipients of Var ivax. Postvaccination seropositivity rates for antibodies against all 4 
viruses were nearly 100% in all 3 groups. Small fold increases were observed for 
measles, mumps, and rubella antibody titers. In contrast, substantial boosts in 
varicella antibody titers were observed among recipients of a second dose of 
varicella vaccine, administered as ProQuad or Varivax.  
o ProQuad may be used in place of a second dose of M -M-RII or second doses of M -
M-RII and Varivax for 4 - to 6-year-old children.  
• Shinefield H, Black S, Thear M, et al. Safety and immunogenicity of a measles, mumps, 
rubella and varicella vaccine given with combined Haemophilus influenzae type b 
conjugate/hepatitis B vaccines and combined diphtheria -tetanus -acellular pertussis 
vaccine s. Pediatr Infect Dis J . 2006;25(4):287 -292. 
doi:10.1097/01.inf.0000207857.10947.1f  
o In this open, multicenter trial, 1915 healthy children ages 12 -15 months were 
randomized into 3 groups: group 1, MMRV, combined Haemophilus influenzae 
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type b conjugate -hepatitis B vaccines (Hib/HepB) and combined diphtheria -
tetanus -acellular pertussis vaccines (DTaP) concomitantly; group 2, MMRV 
followed by Hib/HepB and DTaP 42 days later; group 3, MMR and varicella 
vaccine followed by Hib/HepB and DTaP 42 days later.  
o Antibody responses to measles, mumps, rubella, varicella, Hib, HepB, diphtheria 
and tetanus were similar between groups 1 and 2 (all >95%, except varicella, 
89.7% in group 1 and 90.9% in group 2). Pertussis toxin and filamentous 
hemagglutinin responses wer e significantly lower in group 1 than in group 2 
(group 1, 74.1 and 67.1%; group 2, 90.4 and 86.8%, respectively). An exploratory 
analysis suggested that the difference in pertussis toxin and filamentous 
hemagglutinin responses was likely the result of stu dy design rather than 
interference among vaccine components because the groups differed in age of 
receipt of DTaP (group 1, approximately 12 months; group 2, approximately 13.5 
months). When the groups were matched for age, sample size was sufficient for 
comparison only in children > or =13.5 months old. Pertussis toxin and 
filamentous hemagglutinin responses were similar in these children. The safety 
profiles for each vaccination regimen were comparable.  
o Concomitant administration of MMRV, Hib/HepB and DTaP is well -tolerated.  
• Leonardi M, Bromberg K, Baxter R, et al. Immunogenicity and safety of MMRV and PCV -7 
administered concomitantly in healthy children.  Pediatrics . 2011;128(6):e1387 -e1394. 
doi:10.1542/peds.2010 -2132  
o Healthy 12 - to 15 -month -old children who lacked vaccination and clinical 
histories for measles, mumps, rubella, varicella, and zoster but had written 
documentation of receipt of a 3 -dose primary series of PCV -7 were randomly 
assigned in a 2:1:1 ratio to re ceive either the MMRV and PCV -7 (group 1), PCV -7 
followed 6 weeks later by MMRV (group 2), or MMRV followed 6 weeks later by 
PCV-7 (group 3). The primary safety analysis was 56 days (28 days after each 
visit). Immunogenicity was evaluated 6 weeks after eac h vaccination.  
o A total of 1027 children were enrolled (group 1: 510; group 2: 258; group 3: 259). 
For all 3 groups, the antibody response rate was ≥96.8% for measles, mumps, 
and rubella, ≥88.0% for varicella -zoster virus, and ≥98.3% for all of the 7 
Streptococcus pneumon iae serotypes. The immune responses to all antigens 
present in MMRV and PCV -7 were similar whether administered concomitantly 
or sequentially. The incidence of local and systemic adverse experiences (AEs) 
was comparable between group 1 and groups 2 and 3 c ombined. No vaccine ‐
related serious AEs were reported.  
o Concomitant administration of the MMRV and PCV -7 is highly immunogenic and 
generally well tolerated . Similar immune responses between the groups support 
concomitant administration of the MMRV and PCV -7 to healthy children 12 to 15 
months of age.  
12 
 
• Klein NP , Shepard J, Bedell L, Odrljin T, Dull P . Immunogenicity and safety of a 
quadrivalent meningococcal conjugate vaccine administered concomitantly with 
measles, mumps, rubella, varicella vaccine in healthy toddlers.  Vaccine . 
2012;30(26):3929 -3936. doi:10.1016/j.vaccine.2012.03.080  
o Should be noted that for this study, because of a shortage of Pro Quad during 
part of the study, MMR+V was used.  When “MMRV” is used in this paper, it 
includes subjects vaccinated with MMRV or MMR+V.  
o Two age groups were concurrently enrolled: 7 - to 9-month -old infants who 
received 2 doses of MenACWY -CRM at 7 -9 and 12 months and were randomized 
1:1 to receive MenACWY -CRM with or without MMRV at 12 months, and 12 -
month -old infants who received MMRV only at 12 months. Using predefined 
non-inferiority criteria, immune responses to the antigens in MMRV were 
compared between those who did and did not receive MenACWY -CRM; immune 
responses to MenACWY -CRM as measured by the percentage of subjects with 
human seru m bactericidal activity (hSBA) titers ≥ 8, were compared between 
those who did and did not receive concomitant MMRV. Adequacy of the immune 
response to 2 doses of MenACWY -CRM administered at 7 -9 and 12 months was 
also assessed. Local and systemic reactions , adverse events resulting in 
withdrawal or requiring medical attention and serious adverse events were 
monitored.  
o Concomitant administration of MMRV with MenACWY -CRM did not affect the 
immune response to either vaccine. The 2 -dose series of MenACWY -CRM 
induced adequate immune response to all 4 serogroups. No increased 
reactogenicity was observed with MenACWY ‐CRM+MMRV compared with 
MMRV alone, and there were no study ‐related serious adverse events.  
o Concomitant administration of MenACWY -CRM with MMRV vaccinations at 12 
months was well -tolerated, without safety concerns. Robust immune responses 
to all components of both vaccines were produced and all criteria for non -
inferiority were met, supporting th e use of a 2 -dose regimen of MenACWY -CRM 
in this age group.