NME Mouse Core
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Principal Investigator: STEPHEN C JAMESON Organization: UNIVERSITY OF MINNESOTA Fiscal Year: 2020 Award: $192,500 Funding agency: National Institute of Allergy and Infectious Diseases Summary Core B NME Mouse Core Our published studies suggest that mice with more physiological exposure to natural mouse microbes have immune systems that are a better match for the immune system in humans: In contrast to typical “clean” laboratory mice (which are maintained in barrier facilities to avoid pathogen exposure) these “dirty” mice – also called normal microbial experience (NME) mice – may therefore be more useful to model the characteristics and function of the human immune system, including the maintenance and breakdown of immune tolerance. The goal of this NME Mouse Core is to reliably and cost-effectively produce NME animals that can be used by the Projects in this P01, including careful quality control of these animals to make sure that they are suitably well defined for interpretable studies. Terms: <0-4 weeks old><21+ years old><Abscission><Address><Adult><Adult Human><Animal Housing><Animals><Assay><Autoantigens><Autologous Antigens><BSL-3 facility><BSL3 facility><Bacteria><Bioassay><Biologic Assays><Biological Assay><Birth><Blood><Blood Reticuloendothelial System><Body Tissues><Cell Body><Cell Isolation><Cell Segregation><Cell Separation><Cell Separation Technology><Cells><Characteristics><Containment><Control Animal><Cyclicity><Drug or chemical Tissue Distribution><Excision><Exhibits><Exposure to><Expression Profiling><Extirpation><Gene Expression><Generations><Goals><Guidelines><Heterogeneity><House mice><Human><Human Resources><IACUC><Immune><Immune Tolerance><Immune response><Immune system><Immunes><Immunization><Immunochemical Immunologic><Immunofluorescence><Immunofluorescence Immunologic><Immunohistochemistry><Immunohistochemistry Cell/Tissue><Immunohistochemistry Staining Method><Immunologic><Immunologic Model><Immunologic Sensitization><Immunologic Stimulation><Immunologic Tolerance><Immunological><Immunological Models><Immunological Sensitization><Immunological Stimulation><Immunological response><Immunologically><Immunologics><Immunostimulation><Inbred Mouse><Inbred Strain><Inbred Strains Mice><Inbreeding><Infection><Institutional Animal Care and Use Committee><Investigators><Laboratories><Laboratory mice><Logistics><Lymphocyte><Lymphocytic><Lymphoid><Maintenance><Manpower><Mice><Mice Mammals><Microbe><Modeling><Modern Man><Monitor><Murine><Mus><Mus musculus><Myelogenous><Myeloid><Newborn Infant><Newborns><PBMC><Parasites><Parasitology><Parturition><Periodicity><Peripheral><Peripheral Blood Mononuclear Cell><Phenotype><Physiologic><Physiological><Population><Procedures><Protocol><Protocols documentation><Publishing><Quality Control><Removal><Research Personnel><Research Resources><Researchers><Resistance><Resources><Rhythmicity><Role><Self Tolerance><Self-Antigens><Serologic><Serological><Structure><Surgical Removal><Suspension substance><Suspensions><System><T-Cells><T-Lymphocyte><Testing><Time><Tissue Distribution><Tissues><Training><Transgenic Organisms><Virus><Work><adulthood><allergic/immunologic body system><allergic/immunologic organ system><biosafety level 3 facility><cell sorting><congenic><cost><cost effective><experience><experiment><experimental research><experimental study><germ free condition><host response><immune system tolerance><immune unresponsiveness><immunological paralysis><immunological status><immunoresponse><institutional biosafety committee><knockout gene><lymph cell><member><microbial><newborn child><newborn children><novel><pathogen><pathogen exposure><personnel><resection><resistant><social role><specific pathogen free><thymus derived lymphocyte><transgenic>