Document text
Principal Investigator: Emre H. Brookes
Organization: UNIVERSITY OF MONTANA
Fiscal Year: 2023
Award: $293,074
Funding agency: National Institute of General Medical Sciences
Project Summary/Abstract
We propose to continue development and support for the UltraScan-III (US3) software suite and develop new
code and methods to address challenging biomedical research questions that can be solved with the latest
instruments available for analytical ultracentrifugation and at beamlines for small-angle X-ray and neutron
scattering experiments (SAXS/SANS) experiments. UltraScan is a comprehensive toolkit for the analysis of
data from hydrodynamic experiments and simulations. Such experiments include analytical ultracentrifugation,
SAXS/SANS, as well as bead modeling simulations. Support for this project will assure continued availability of
a mature multi-platform analysis suite with important and unique capabilities not found in any other software
packages. Chief among them are tight integration of supercomputing capabilities, LIMS support for
collaboration, and specialized analysis routines. US3 enjoys widespread use in the AUC and SAXS/SANS
communities, and offers the most robust optimization and simulation algorithms available, resulting in
unmatched detail. UltraScan provides the highest throughput, a flexible, modern GUI, and a comprehensive list
of analysis routines. Proposed developments include automated data acquisition and analysis workflows,
improvements in the fitting of floating data, improved two-dimensional grids, support for density distribution
analysis critical for the accurate characterization and quantification of viral vectors, lipid nanoparticles loaded
with cancer drugs or nucleic acids, and other vectors used for the delivery of medicines or CRISPR technology.
Terms: <2-dimensional><AIDS Virus><APBA1><APBA1 gene><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome Virus><Address><Aging><Algorithms><Amyloid Beta A4 Precursor Protein-Binding, Family A, Member 1><Anisotropy><Anti-Cancer Agents><Antineoplastic Agents><Antineoplastic Drugs><Antineoplastics><Architecture><Australia><Biochemistry><Biological Chemistry><Biomedical Research><Biophysics><Biopolymers><CRISPR approach><CRISPR based approach><CRISPR method><CRISPR methodology><CRISPR technique><CRISPR technology><CRISPR tools><CRISPR-CAS-9><CRISPR-based method><CRISPR-based technique><CRISPR-based technology><CRISPR-based tool><CRISPR/CAS approach><CRISPR/Cas method><CRISPR/Cas technology><CRISPR/Cas9><CRISPR/Cas9 technology><Canada><Cancer Drug><Cancers><Cas nuclease technology><Cellular biology><Clustered Regularly Interspaced Short Palindromic Repeats approach><Clustered Regularly Interspaced Short Palindromic Repeats method><Clustered Regularly Interspaced Short Palindromic Repeats methodology><Clustered Regularly Interspaced Short Palindromic Repeats technique><Clustered Regularly Interspaced Short Palindromic Repeats technology><Code><Coding System><Collaborations><Communities><Complex><Computer software><D9S411E><Data><Data Analyses><Data Analysis><Data Bases><Data Protection><Data Security><Data awareness><Databases><Degenerative Neurologic Diseases><Degenerative Neurologic Disorders><Development><Diffusion><Dimensions><Disease><Disorder><Drug Targeting><Engineering / Architecture><Ensure><Europe><Finland><Fluorescence><Funding><Future><HIV><High Performance Computing><Human Immunodeficiency Viruses><India><Infrastructure><Institution><Intuition><Investigation><Kinetics><LAV-HTLV-III><LIN10><Laboratories><Libraries><Licensing><Linux><Lymphadenopathy-Associated Virus><MINT1><MUNC18-1-Interaction Protein><Macromolecular Structure><Maintenance><Malignant Neoplasms><Malignant Tumor><Management Information Systems><Measures><Medicine><Methods><Modernization><Molecular><Molecular Structure><NIH><National Institutes of Health><Neoplastic Disease Chemotherapeutic Agents><Nervous System Degenerative Diseases><Network-based><Neural Degenerative Diseases><Neural degenerative Disorders><Neurodegenerative Diseases><Neurodegenerative Disorders><Neurologic Degenerative Conditions><Neutrons><Nucleic Acids><Operating System><Peer Review><Property><Publications><Reaction><Records><Research><Research Resources><Resolution><Resources><Roentgen Rays><Science><Scientific Publication><Services><Short interfering RNA><Side><Single Crystal Diffraction><Small Interfering RNA><Social Support System><Software><Spectroscopy><Spectrum Analyses><Spectrum Analysis><Supercomputing><Support System><System><Systems Analyses><Systems Analysis><Techniques><Training><Tumor-Specific Treatment Agents><United States National Institutes of Health><Update><Vertebrate LIN10 Homolog><Viral Vector><Virus-HIV><Work><X Ray Crystallographies><X-Radiation><X-Ray Crystallography><X-Ray Diffraction Crystallography><X-Ray Radiation><X-Ray/Neutron Crystallography><X-ray><X11><X11-Alpha><X11-α><Xray><Xray Crystallography><analytical ultracentrifugation><anti-cancer drug><anticancer agent><anticancer drug><automated analysis><base><bases><beamline><biophysical analysis><biophysical foundation><biophysical principles><biophysical sciences><biophysical studies><cell biology><code development><data acquisition><data acquisitions><data base><data interpretation><degenerative diseases of motor and sensory neurons><degenerative neurological diseases><density><design><designing><develop software><developing computer software><developmental><diffused><diffuses><diffusing><diffusions><drug detection><drug testing><experiment><experimental research><experimental study><experiments><flexibility><flexible><high throughput analysis><high-end computing><improved><information security><instrument><instrumentation><intuitive><lipid based nanoparticle><lipid nanoparticle><macromolecular assembly><malignancy><materials science><middleware><model-based simulation><models and simulation><nano science><nanoscience><neoplasm/cancer><neurodegenerative illness><novel><open source><operation><operations><resolutions><siRNA><simulation><software development><structural biology><super computing><supercomputer><therapeutic agent development><therapeutic development><tool><two-dimensional><user protection><vector>