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Principal Investigator: Ananth V Annapragada
Organization: BAYLOR COLLEGE OF MEDICINE
Fiscal Year: 2020
Award: $156,621
Funding agency: Eunice Kennedy Shriver National Institute of Child Health and Human Development
An understanding of the transplacental transport of the SARS-CoV-2 virion is of enormous importance.
Objective characterization of virion transport, even in animal models, is a hugely challenging task. Using
transplacental transport as an example, we seek to address this problem: we will design and build a Virion
Surrogate Particle (VSP). Binding of the VSP to target receptors (ACE2), and subsequent internalization will
be validated in cell culture, followed by in vivo testing of transplacental transport. The specific aims for this
supplement are thereore:
1. Prepare Virus Surrogate Particles (VSP), recapitulating the viral lipid envelope, bearing the Spike
protein S on the surface and carrying proteolytic Furin in the lumen, and validate binding of VSP to
the ACE2 receptor in cell culture studies.
2. Study trans-placental transport of VSP in the k18-hACE2 mouse model with the humanized ACE2
receptor throughout the course of pregnancy using MR, CT and fluorescence tracing of particles
3. Study the effect of known ACE2 receptor level effectors (low protein diet, hypertension, high fat diet)
on transplacental transport of VSP
Terms: <1st trimester><2019 novel coronavirus><2019-nCoV><3rd trimester><AD dementia><Address><Alzheimer><Alzheimer Type Dementia><Alzheimer disease><Alzheimer sclerosis><Alzheimer syndrome><Alzheimer's><Alzheimer's Disease><Alzheimer's disease dementia><Alzheimers Dementia><Alzheimers disease><Amyloid Plaques><Animal Model><Animal Models and Related Studies><Antibodies><Award><BSL-3 facility><BSL3 facility><Basic Research><Basic Science><Bears><Binding><Biodistribution><Blood Vessels><Body Tissues><COVID-19><COVID19><Capsules><Cell Culture Techniques><Cells Placenta-Tissue><Characteristics><Clinical Trials><Cofactor Protein S><Communities><Data><Dependence><Detection><Development><Disease><Disorder><Early Placental Phase><Envelope Protein><Fetus><First Pregnancy Trimester><First Trimester><Fluorescence><Gestation><Grant><High Fat Diet><Hypertension><Image><Infection><Investigators><Last Trimester><Lentivirinae><Lentivirus><Life><Lipids><Lipoproteins><Low-Protein Diet><Lung diseases><Lung infections><MR Imaging><MR Tomography><MRI><Magnetic Resonance Imaging><Medical Imaging, Magnetic Resonance / Nuclear Magnetic Resonance><Miscarriage><Molecular><Molecular Interaction><Mothers><Myocarditis><NMR Imaging><NMR Tomography><Neuritic Plaques><Normal Placentoma><Nuclear Magnetic Resonance Imaging><Nucleocapsid><Paper><Patients><Pediatric Radiology><Phosphoproteins><Placenta><Placenta Accreta><Placenta Embryonic Tissue><Placentome><Pneumonia><Pregnancy><Premature Birth><Prematurely delivering><Preterm Birth><Primary Senile Degenerative Dementia><Protein S><Protein-Restricted Diet><Proteins><Proteome><Public Health><Pulmonary Diseases><Pulmonary Disorder><Receptor Protein><Reporting><Research><Research Personnel><Researchers><Respiratory Disease><Respiratory System Disease><Respiratory System Disorder><Rest><Risk><Route><SARS Virus><SARS corona virus><SARS coronavirus><SARS-Associated Coronavirus><SARS-CoV><SARS-CoV-2><SARS-CoV2><SARS-Related Coronavirus><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related coronavirus 2><Senile Plaques><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe acute respiratory syndrome coronavirus 2><Site><Societies><Spontaneous abortion><Subfamily lentivirinae><Surface><Third Pregnancy Trimester><Third Trimester><Time><Tissues><Training><Transmission><Ursidae><Ursidae Family><Vascular Hypertensive Disease><Vascular Hypertensive Disorder><Viral><Virion><Virus><Virus Particle><Virus-Lenti><Visualization><Vitamin K-Dependent Protein S><Work><Wuhan coronavirus><Zeugmatography><amyloid beta plaque><amyloid-b plaque><aβ plaques><bear><biosafety level 3 facility><capsule><cardiac inflammation><cell culture><cored plaque><corona virus disease 2019><coronavirus disease 2019><dementia of the Alzheimer type><design><designing><developmental><diffuse plaque><disease of the lung><disorder of the lung><env Antigens><env Gene Products><env Polyproteins><env Protein><experience><exposure route><fetal infection><folate carrier><folate receptor><folate-binding protein><folate-methotrexate transporter><folic acid binding protein><folic acid receptor><high blood pressure><hyperpiesia><hyperpiesis><hypertensive disease><imaging><imaging agent><in vivo><in vivo evaluation><in vivo testing><lipid nanoparticle><lung disorder><meetings><methotrexate-binding protein><model of animal><model organism><molecular imaging><molecule imaging><mouse model><murine model><particle><premature childbirth><premature delivery><preterm delivery><primary degenerative dementia><pulmonary infections><receptor><respiratory><senile dementia of the Alzheimer type><severe acute respiratory syndrome-CoV><transmission process><vascular><viral envelope lipids>