Document text
Principal Investigator: Ellen I Hartig
Organization: TUFTS UNIVERSITY BOSTON
Fiscal Year: 2024
Award: $44,724
Funding agency: National Institute on Deafness and Other Communication Disorders
PROJECT SUMMARY/ABSTRACT
Our ability to hear relies on a small population of specialized sensory ‘hair’ cells in the inner ear that
cannot regenerate upon damage. Variants of over 100 human genes have been associated with deafness,
many of which alter the structure of the sensory organelle in hair cells, called the hair bundle. The hair bundle
consists of organized rows of graded-height membrane protrusions, or stereocilia, on the surface of hair cells.
Hair bundle formation during development and its precise architecture during life are vulnerable to a
variety of genetic, environmental, and age-related insults, all of which result in the decline or complete loss
of hearing ability. My overarching goal is to characterize protein interactions that are vital to the hair bundle’s
development and lifelong maintenance. In this proposal, I identify a new role for a calcium-binding
protein enriched in the mouse hair bundle, specifically, at the tip of the tallest stereocilia. A group of five
proteins, referred to as the Elongation Complex, was previously reported at this same compartment. Loss of
any member of this complex prevents proper stereocilia elongation, blurs the distinct identity of stereocilia
across rows, and results in profound deafness in both humans and mice. Interestingly, similar defects have
been reported in mouse mutants lacking key components of the mechanoelectrical transduction channel. This
suggests that, via transduction, active hair bundles somehow influence elongation factors, and thus
stereocilia dimensions. I propose that the calcium-binding protein studied in this proposal is a new binding
partner and regulator for MYO15A, the myosin motor that transports other Elongation Complex proteins to
stereocilia tips. MYO15A was one of the first proteins associated with hearing loss, and MYO15A mutations
are the third most common origin of heredity deafness in humans. Aim 1 of this proposal will determine the
relationship between MYO15A and our calcium-binding protein, and confirm preliminary results suggesting
that this new protein is essential for auditory function. Aim 2 will investigate how changes in calcium levels
in the hair bundle upon transduction affect the dynamic localization of our protein and its Elongation
Complex partners. Together, these aims identify and investigate this new stereocilia protein as an
additional member of the crucial Elongation Complex, and as a mediator that reads hair cell transduction
activity to influence stereocilia growth. This project employs cutting- edge mouse models, advanced
techniques to culture the auditory organ, and high-resolution imaging of preserved and live hair cells.
Completion of the project will contribute to my long-term goal of informing therapeutic strategies to ensure
the proper development and lifelong preservation of hair cells.
Terms: <21+ years old><Ablation><Actin-Activated ATPase><Actins><Adult><Adult Human><Affect><Architecture><Audiogram><Audiometric Test><Audiometry><Auditory><Aves><Avian><Bactrim><Basic Research><Basic Science><Behavior><Bilateral Deafness><Binding><Biology><Birds><CRISPR approach><CRISPR based approach><CRISPR method><CRISPR methodology><CRISPR technique><CRISPR technology><CRISPR tools><CRISPR-CAS-9><CRISPR-based method><CRISPR-based technique><CRISPR-based technology><CRISPR-based tool><CRISPR/CAS approach><CRISPR/Cas method><CRISPR/Cas technology><CRISPR/Cas9><CRISPR/Cas9 technology><Calcium><Calcium ion><Calcium-Binding Proteins><Cas nuclease technology><Cellular Mechanotransduction><Centrin><Clustered Regularly Interspaced Short Palindromic Repeats approach><Clustered Regularly Interspaced Short Palindromic Repeats method><Clustered Regularly Interspaced Short Palindromic Repeats methodology><Clustered Regularly Interspaced Short Palindromic Repeats technique><Clustered Regularly Interspaced Short Palindromic Repeats technology><Cochlea><Cochlear Organ><Complete Deafness><Complete Hearing Loss><Complex><Corti Cell><Cotrim><Data><Defect><Development><Dimensions><Drugs><Dysmorphology><EF-Hand Calcium-Binding Motif><EF-Hand Domain><Elements><Elongation Factor><Engineering / Architecture><Ensure><Environment><Eslectin><Generalized Growth><Genes><Genetic><Genetic Alteration><Genetic Change><Genetic defect><Goals><Growth><Hair><Hair Cells><Hearing><Hearing Loss><Hearing Tests><Height><Heredity><Hour><Human><Hypoacuses><Hypoacusis><Image><Insozalin><Internal Ear><Isoforms><KO mice><Knock-out Mice><Knockout Mice><Knowledge><Labyrinth><Letters><Life><Light><Link><Maintenance><Mechanical Signal Transduction><Mechanosensory Transduction><Mediating><Mediator><Medication><Membrane><Mice><Mice Mammals><Modern Man><Molecular Configuration><Molecular Conformation><Molecular Interaction><Molecular Stereochemistry><Morphogenesis><Morphology><Motor><Murine><Mus><Mutant Strains Mice><Mutate><Mutation><Myosin ATPase><Myosin Adenosine Triphosphatase><Myosin Adenosinetriphosphatase><Myosins><Natural regeneration><Null Mouse><Organ><Organelles><Pattern><Pharmaceutical Preparations><Phenotype><Photoradiation><Population><Presbyacusis><Presbycusis><Preventive><Protein Isoforms><Proteins><RNA Splicing><Regeneration><Reporting><Research><Role><SMZ-TMP><Sensory><Sensory Hair><Splicing><Structure><Surface><TMP-SMX><Techniques><Testing><Therapeutic><Time><Tissue Growth><Total Deafness><Transgenic Mice><Trimedin><Trimethoprim-Sulfamethoxazole><Trimezole><Variant><Variation><Work><adulthood><age associated><age associated hearing loss><age correlated><age dependent><age induced hearing loss><age linked><age related><age related decline in hearing><age related hearing deficits><age related hearing impairment><age related hearing loss><age specific><aging associated hearing loss><aging induced hearing loss><aging related decline in hearing><aging related hearing deficits><aging related hearing impairment><aging related hearing loss><antibody based detection><antibody detection><auditory tests><cell transduction><cell type><cellular transduction><conformation><conformational><conformational state><conformationally><conformations><congenital deafness><congenitally deaf><deaf><deafened><deafness><detect antibodies><developmental><drug/agent><dysfunctional hearing><ear hair cell><experiment><experimental research><experimental study><experiments><genome mutation><hearing assessment><hearing challenged><hearing defect><hearing deficient><hearing deficit><hearing difficulty><hearing dysfunction><hearing impairment><high resolution imaging><imaging><in vivo><inner ear><mechanical stimulus><mechanosensing><mechanotransduction><member><membrane structure><morphogenetic process><mouse model><mouse mutant><murine model><mutant><novel><ontogeny><pharmacologic><postnatal><preservation><prevent><preventing><profound hearing loss><protein complex><regenerate><social role><sound><transduced cells>