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Principal Investigator: Beth Ann Tamburini
Organization: UNIVERSITY OF COLORADO DENVER
Fiscal Year: 2022
Award: $188,825
Funding agency: National Institute of Allergy and Infectious Diseases
Project Summary
Live attenuated vaccinations generate both humoral and cellular immune memory, accounting for much of the
increased duration of protective immune memory. As increased protective immune memory to live attenuated
vaccines is of critical importance, understanding the mechanisms of this increased protective immune memory
is essential to improve current vaccines. To this end, we and others have demonstrated that antigens derived
from infectious viral infections persist in the host for extended periods of time, well beyond the time in which
the infection is cleared from the host. Our lab has specifically identified that antigens derived from both
vaccination and viral infections persist or are archived by the host lymphatic endothelial cells LECs, identifying
the source of archived antigens. We have published that this archived antigen maintains a more effector like
pool of antigen specific memory cells which enhances the clearance of a secondary infectious challenge. Thus,
identification of key mechanisms involved in antigen archiving during vaccination is critical for our
understanding of enhanced protective immunity to vaccination. To better understand the mechanisms of
antigen archiving we have developed a “molecular tracking device” that leverages single-cell mRNA
sequencing to track the distribution, acquisition, and retention of antigen in the lymph node and other organs.
This project will elucidate the unique mechanisms behind antigen archiving, how multiple un-related infections
contribute to the kinetics of archived antigens and memory boosting, and the potential cell types in other
tissues that may also contribute to antigen archiving.
Terms: <Accounting><Address><Adjuvant><Antigen Presentation><Antigens><Archives><Attenuated><Attenuated Vaccines><Body Tissues><CD8 Cell><CD8 T cells><CD8 lymphocyte><CD8+ T cell><CD8+ T-Lymphocyte><CD8-Positive Lymphocytes><CD8-Positive T-Lymphocytes><Cell Body><Cell Communication><Cell Death><Cell Interaction><Cell Mediated Immunology><Cell-Mediated Immunity><Cell-to-Cell Interaction><Cells><Cellular Immunity><DNA><Data><Dendritic Cells><Deoxyribonucleic Acid><Devices><Gene Expression><Immune memory><Immunity><Immunologic Memory><Immunological Memory><Individual><Infection><Kinetics><Label><Lead><Live-attenuated Vaccine><Lymph Node Reticuloendothelial System><Lymph node proper><Lymphatic Endothelial Cells><Lymphatic nodes><Memory><Methods><Mice><Mice Mammals><Modeling><Molecular><Murine><Mus><Oligo><Oligonucleotides><Organ><Organ System><Pb element><Peripheral><Process><Proteins><Publishing><Regimen><Resistance><Reticular Cell><Route><SCmRNAseq><Source><Stromal Cells><System><T cell response><T memory cell><T-Cells><T-Lymphocyte><T8 Cells><T8 Lymphocytes><Techniques><Time><Tissues><Vaccinated><Vaccination><Vaccines><Veiled Cells><Viral><Viral Antigens><Viral Diseases><Virus><Virus Diseases><anamnestic reaction><body system><cell type><functional outcomes><heavy metal Pb><heavy metal lead><immunization strategy><immunogen><improved><live vaccine><live vaccines><lymph gland><lymph nodes><lymphnodes><memory T lymphocyte><necrocytosis><new approaches><novel><novel approaches><novel strategies><novel strategy><nuclease><oligos><programs><resistant><secondary immune response><single cell mRNA sequencing><thymus derived lymphocyte><uptake><vaccination strategy><vaccine formulation><viral infection><virus antigen><virus infection><virus-induced disease>