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Principal Investigator: Normand Leblanc
Organization: UNIVERSITY OF NEVADA RENO
Fiscal Year: 2024
Award: $338,442
Funding agency: National Institute of General Medical Sciences
Project Summary – High Spatial and Temporal Resolution Imaging (HSTRI) Core (Core C)
The goal of the present proposal is to maintain and enhance a High Spatial and Temporal Resolution Imaging
Core (HSTRI) research core (Core C) that offers a wide array of state-of-the-art instruments enabling
visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators.
With the assistance of expert staff and unrestricted access to these instruments, greatly enhanced the
competitiveness of Research Project Leaders (RPLs) in Phase I, and we will provide the same services to RPLs,
pipeline investigators, and Pilot Project leaders in Phase II. This facility is specifically designed to meet the needs
of our RPLs but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno.
Core C will accomplish the following Specific Aims during Phase II of the COBRE: Specific Aim 1: To operate
and expand a facility providing state-of-the-art high spatial and temporal resolution imaging
methodologies for studying cellular structure, signaling pathways, and function in health and disease.
Recent advances in microscopy have produced a new generation of commercially available instruments that
enable biomedical researchers to investigate the relationships between structure and function in biological
systems with unprecedented spatial and temporal resolution. The main goal of this Specific Aim is to continue
making advanced imaging modalities available to COBRE investigators and to the larger UNR community of
researchers. Specific Aim 2: To continue advising, training, and informing investigators in the use of the
new technologies. Training users in the operation of HSTRI Core equipment and appropriate sample
preparation, as well as educating them about the scientific possibilities offered by these technologies, is crucial
to maximizing the Core’s impact on research and for achieving long-term self-sustainability. Core personnel will
continue to provide appropriate training and supervision of users, manage the logistical challenges associated
with accommodating multiple research projects, and offer workshops on novel microscopy and imaging
approaches. Specific Aim 3: To expand the userbase and devise an operational plan that will sustain the
long-term viability of the Core. Plans were already implemented during Phase I and will continue to be
developed to provide support for the Core after the end of the funded period through a business plan of revenues
that includes user fees and institutional support. As was the case for Phase I, HSTRI Core services will continue
to be offered to investigators directly associated with the COBRE (Research and Pipeline Project Leaders, and
Senior COBRE investigators) and non-COBRE investigators at times not reserved by COBRE investigators.
Notable in this latter context, many well-funded laboratories at UNR Med and UNR, including non-biological labs
in Engineering, have begun utilizing the infrastructure to incorporate high-resolution imaging methods in their
research and grant applications.
Terms: <3-D><3-Dimensional><3D><Acceleration><Administrative Supplement><Applications Grants><Award><Biomedical Research><Biosensor><Body Tissues><Businesses><COBRE><COVID crisis><COVID epidemic><COVID pandemic><COVID-19 crisis><COVID-19 epidemic><COVID-19 era><COVID-19 global health crisis><COVID-19 global pandemic><COVID-19 health crisis><COVID-19 pandemic><COVID-19 period><COVID-19 public health crisis><COVID-19 years><Cardiovascular><Cardiovascular Body System><Cardiovascular Organ System><Cardiovascular system><Cell Body><Cell Communication and Signaling><Cell Components><Cell Signaling><Cell Structure><Cells><Cellular Structures><Center of Biomedical Research Excellence><Centers of Research Excellence><Communities><Computer Analysis><Computer software><Core Facility><Coupled><Development><Disease><Disorder><Educational workshop><Engineering><Equipment><FRET><Fees><Fluorescence Resonance Energy Transfer><Foundations><Funding><Future><Förster Resonance Energy Transfer><Generations><Goals><Grant><Grant Proposals><Health><Heart Vascular><Human Resources><Image><Image Analyses><Image Analysis><Individual><Infrastructure><Institution><International><Intracellular Communication and Signaling><Investigators><Investments><Label><Laboratories><Laser Electromagnetic><Laser Radiation><Lasers><Licensing><Light><Manpower><Methodological Studies><Microscope><Microscopy><Microtome - medical device><Molecular><Nevada><Paper><Performance><Pharmacology><Phase><Photoradiation><Pilot Projects><Preparation><R-Series Research Projects><R01 Mechanism><R01 Program><Research><Research Grants><Research Personnel><Research Project Grants><Research Projects><Researchers><SARS-CoV-2 epidemic><SARS-CoV-2 global health crisis><SARS-CoV-2 global pandemic><SARS-CoV-2 pandemic><SARS-coronavirus-2 epidemic><SARS-coronavirus-2 pandemic><Sampling><Scanning><Services><Severe Acute Respiratory Syndrome CoV 2 epidemic><Severe Acute Respiratory Syndrome CoV 2 pandemic><Severe acute respiratory syndrome coronavirus 2 epidemic><Severe acute respiratory syndrome coronavirus 2 pandemic><Signal Pathway><Signal Transduction><Signal Transduction Systems><Signaling><Software><Structure><Supervision><System><Technology><Tissue Sample><Tissues><Training><Universities><Visualization><Workshop><base><bases><biological sensor><biological signal transduction><biological systems><circulatory system><computational analyses><computational analysis><computer analyses><coronavirus disease 2019 crisis><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 pandemic><coronavirus disease 2019 public health crisis><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease pandemic><coronavirus disease-19 global pandemic><coronavirus disease-19 pandemic><cryostat><design><designing><developmental><experiment><experimental research><experimental study><experiments><high end computer><high performance computer><high resolution imaging><image evaluation><image interpretation><image-based method><imaging><imaging approach><imaging based approach><imaging capabilities><imaging method><imaging modality><imaging system><instrument><instrumentation><medical college><medical schools><member><microtome><new technology><novel><novel technologies><operation><operations><patch clamp><personnel><pharmacologic><pilot study><preparations><school of medicine><severe acute respiratory syndrome coronavirus 2 global health crisis><severe acute respiratory syndrome coronavirus 2 global pandemic><success><super high resolution><superresolution><temporal measurement><temporal resolution><three dimensional><time measurement><ultra high resolution>