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Principal Investigator: Neil King
Organization: UNIVERSITY OF WASHINGTON
Fiscal Year: 2022
Award: $958,363
Funding agency: National Institute of Allergy and Infectious Diseases
PROJECT SUMMARY – CORE A: PROTEIN SCIENCES
Core A (Protein Sciences) will provide essential support to our Projects and Cores in three areas: protein
production and characterization, high-resolution structure determination, and process development and
technology transfer. Core A’s protein production and characterization efforts, led by Dr. Neil King and Lauren
Carter, will leverage the extensive facilities and equipment at UW’s Institute for Protein Design (IPD) as well as
its unique expertise in nanoparticle immunogens. Rigorous characterization and quality control testing will ensure
the provision of high-quality antigens, nanoparticle immunogens, and protein-based reagents to support the
scientific efforts of Projects 1 (Veesler), 2 (King), and 3 (Pulendran). Structure determination by both X-ray
crystallography and cryo-electron microscopy will be led by Dr. David Veesler and will make use of state-of-the-
art, in-house facilities including UW’s Arnold and Mabel Beckman Cryo-EM Center. High-resolution structures of
designed antigens, nanoparticle immunogens, and antigen-antibody complexes will provide critical information
to guide the iterative design of stabilized antigens and broadly protective vaccines in Project 2. Process
development and technology transfer of our lead vaccine candidates (a pan-sarbecovirus vaccine at the end of
Year 2 and a pan-betacoronavirus vaccine at the end of Year 5), led by Dr. Neil King and Lauren Carter, will
build upon the IPD’s proven track record in translating innovative protein-based medicines and vaccines to
industry partners. A dedicated team at the IPD will develop the methods and documentation for a comprehensive
technology transfer package that will speed preclinical and clinical development of our broadly protective
coronavirus vaccines.
Terms: <Antigen Binding Fragment><Antigen-Antibody Complex><Antigenic Determinants><Antigens><Area><Assay><B blood cells><B cell><B cells><B-Cells><B-Lymphocytes><B-cell><Binding><Binding Determinants><Bioassay><Biologic Assays><Biological Assay><Bioreactors><Cell Isolation><Cell Segregation><Cell Separation><Cell Separation Technology><Clinical Treatment Moab><CoV S protein><CoV glycoprotein S><CoV spike glycoprotein><CoV spike protein><Collaborations><Complex><Coronaviridae><Coronavirus><Coronavirus glycoprotein S><Coronavirus spike protein><Cryo-electron Microscopy><Cryoelectron Microscopy><Cyclic GMP><Development><Documentation><Electron Cryomicroscopy><Ensure><Epitopes><Equipment><Fab Fragments><Fab Immunoglobulins><Formulation><Glycoproteins><Goals><Grippe><Guanosine Cyclic Monophosphate><HIV-1><HIV-I><HIV1><High Throughput Assay><Human Immunodeficiency Virus Type 1><Human Resources><Human immunodeficiency virus 1><Imaging Procedures><Imaging Technics><Imaging Techniques><Immune Complex><Immunoglobulin, F(ab) Fragment><In Vitro><Influenza><Institutes><Institution><Laboratories><Lead><Manpower><Medicine><Methods><Molecular Interaction><Monoclonal Antibodies><Pb element><Peptides><Process><Production><Protein Engineering><Protein Sortings><Proteins><Public Health><Quality Control><Reagent><Receptor Protein><Reference Standards><Research><Research Support><Resolution><Sarbecovirus><Science><Scientist><Serology><Single Crystal Diffraction><Speed><Structure><System><Technology Transfer><Testing><Time><Translating><Translations><Vaccine Design><Vaccines><Viral><X Ray Crystallographies><X-Ray Crystallography><X-Ray Diffraction Crystallography><X-Ray/Neutron Crystallography><Xray Crystallography><analytical method><base><beta coronavirus vaccine><betacoronavirus vaccine><cGMP><cell sorting><clinical development><corona virus><coronavirus S protein><coronavirus spike glycoprotein><coronavirus vaccine><cost><cryo-EM><cryoEM><design><designing><developmental><experience><experiment><experimental research><experimental study><flexibility><flexible><genetic protein engineering><heavy metal Pb><heavy metal lead><high throughput screening><immunogen><immunogenicity><in vivo><industrial partnership><industry partner><industry partnership><innovate><innovation><innovative><iterative design><mAbs><method development><microbioreactor><nano particle><nano-sized particle><nanoparticle><nanosized particle><panbetaCoV vaccine><personnel><polyclonal antibody><pre-clinical development><preclinical development><programs><protein design><protein structure function><receptor><scale up><structural biology><vaccine against coronavirus><vaccine candidate><β coronavirus vaccine>