Document text
Principal Investigator: William A Schountz
Organization: COLORADO STATE UNIVERSITY
Fiscal Year: 2024
Award: $214,725
Funding agency: National Institute of Allergy and Infectious Diseases
Project Summary / Abstract
COVID-19 is caused by a bat-borne coronavirus, SARS-CoV-2, that has resulted in more than 1 million deaths
in the USA. Experimental and bioinformatic analyses suggest cricetid rodents, but not murid rodents, may be
susceptible to the virus. We determined that two such cricetid rodents, the North American deer mouse
(Peromyscus maniculatus) and California deer mouse (P. californicus) are susceptible, with some of the latter
species developing severe disease that required euthanasia. This raises the concern that spillback from
humans or susceptible domesticated animals, such as mins (Neogale vison), to North American cricetid
rodents could occur and lead to establishment of SARS-CoV-2 in secondary reservoir hosts in the New World.
Several rodent species in Europe and Asia have been found to harbor coronaviruses but, surprisingly, there
are no reports wild rodents in the New World have been examined for coronaviruses, even though they are
found in several bat species. Moreover, because of disease that occurred in California deer mice, it is possible
that it or other North American rodents could serve as new pathogenesis models for COVID-19. To examine
these possibilities, we will survey cricetid rodents in Colorado for the presence of coronaviruses, which we
have detected at a site in Utah, and generate genome sequences of these viruses for phylogenetic analysis
and important domains (e.g., protease cleavage sites). We will also determine the T cell response of deer mice
vaccinated against SARS-CoV-2 using single-cell RNA seq analysis and compared to unvaccinated deer mice.
This will lay the foundation of using deer mice as a long-lived small animal model (8 years), which cannot be
replicated with Syrian hamsters or human ACE2 laboratory mice (2 years). Together this work will determine
coronavirus diversity in North American rodents, and whether the deer mouse can serve as a durable immunity
model for SARS-CoV-2.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><2019-nCoV variant><2019-nCoV variant forms><2019-nCoV variant strains><ACE2><Agreement><American><Animal Model><Animal Models and Related Studies><Antibodies><Antibody titer measurement><Asia><Back><Bats><Bio-Informatics><Bioinformatics><Bovine Species><Budgets><COVID-19><COVID-19 immunity><COVID-19 infection><COVID-19 predisposition><COVID-19 susceptibility><COVID-19 transmission><COVID-19 vaccination><COVID-19 variant><COVID-19 variant forms><COVID-19 variant strains><COVID-19 virus><COVID-19 virus infection><COVID-19 virus transmission><COVID-19 vulnerability><COVID19 infection><COVID19 virus><CV-19><California><Canada><Cattle><Causality><Central America><Cessation of life><Charge><Chiroptera><CoV emergence><CoV pandemic><CoV-2><CoV2><Colorado><Coronaviridae><Coronaviridae Infections><Coronavirus><Coronavirus Infections><Coronavirus Infectious Disease 2019><Coronavirus disease 2019 predisposition><Coronavirus disease 2019 susceptibility><Coronavirus disease 2019 vulnerability><Cost Sharing><DNA Recombination><Data><Death><Deer><Deer Mouse><Development><Disease><Disorder><Domestic Animals><Dorsum><Equipment><Esteroproteases><Etiology><Europe><Euthanasia><Event><Facilities and Administrative Costs><Farm><Fees><Foundations><Genetic Recombination><Genome><Genotype><Golden Hamsters><Golden Syrian Hamsters><Human><Immune response><Immunity><Immunochemical Immunologic><Immunologic><Immunological><Immunological response><Immunologically><Immunologics><Indirect Costs><Laboratory mice><Life><Mammalia><Mammals><Mediation><Mercy Killing><Mesocricetus auratus><Mexico><Mink><Modeling><Modern Man><Negotiating><Negotiation><North America><Pathogenesis><Peptidases><Peptide Hydrolases><Peromyscus><Phylogenetic Analysis><Phylogenetics><Population><Predisposed to COVID-19><Predisposed to SARS-CoV-2><Predisposed to Severe acute respiratory syndrome coronavirus 2><Predisposition><Prevalence><Protease Gene><Proteases><Proteinases><Proteolytic Enzymes><Recombination><Reporting><Research><Resistance><Rodent><Rodentia><Rodents Mammals><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CO-V2><SARS-COVID-2><SARS-CoV><SARS-CoV-1><SARS-CoV-2><SARS-CoV-2 immunity><SARS-CoV-2 infection><SARS-CoV-2 predisposition><SARS-CoV-2 susceptibility><SARS-CoV-2 transmission><SARS-CoV-2 vaccination><SARS-CoV-2 variant><SARS-CoV-2 variant forms><SARS-CoV-2 variant strains><SARS-CoV-2 vulnerability><SARS-CoV2><SARS-CoV2 infection><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Sarbecovirus><Serology><Seroprevalences><Severe Acute Respiratory Coronavirus><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 immunity><Severe acute respiratory syndrome coronavirus 2 infection><Severe acute respiratory syndrome coronavirus 2 predisposition><Severe acute respiratory syndrome coronavirus 2 susceptibility><Severe acute respiratory syndrome coronavirus 2 vaccination><Severe acute respiratory syndrome coronavirus 2 vulnerability><Severe acute respiratory syndrome related corona virus 2><Site><Source><Survey Instrument><Surveys><Susceptibility><Syrian Hamsters><T cell response><T-Cells><T-Lymphocyte><Tail><United States><Universities><Utah><Vaccinated><Vaccination><Vaccines><Virus><Work><Wuhan coronavirus><Zoonoses><Zoonotic><Zoonotic Infection><angiotensin converting enzyme 2><angiotensin converting enzyme II><antibody titering><base><bases><bat-borne><batborne><beta CoV><beta coronavirus><betaCoV><betacoronavirus><bovid><bovine><causation><corona virus><corona virus emergence><coronavirus disease 2019><coronavirus disease 2019 immunity><coronavirus disease 2019 infection><coronavirus disease 2019 transmission><coronavirus disease 2019 vaccination><coronavirus disease 2019 variant><coronavirus disease 2019 variant forms><coronavirus disease 2019 variant strains><coronavirus disease 2019 virus><coronavirus disease 2019 virus transmission><coronavirus disease-19><coronavirus disease-19 virus><coronavirus emergence><coronavirus infectious disease-19><coronavirus pandemic><cost><cow><data management><data sharing><deermouse><developmental><disease causation><domesticated animal><emergent CoV><emergent corona virus><emergent coronavirus><emerging CoV><emerging corona virus><emerging coronavirus><hCoV19><host response><immune system response><immunity against COVID-19><immunity against SARS-CoV-2><immunity against Severe acute respiratory syndrome coronavirus 2><immunity against coronavirus disease 2019><immunity to COVID-19><immunity to SARS-CoV-2><immunity to Severe acute respiratory syndrome coronavirus 2><immunity to coronavirus disease 2019><immunoresponse><infected with COVID-19><infected with COVID19><infected with SARS-CoV-2><infected with SARS-CoV2><infected with coronavirus disease 2019><infected with severe acute respiratory syndrome coronavirus 2><life span><lifespan><model of animal><nCoV><nCoV2><new CoV><new corona virus><new coronavirus><non vaccinated><not vaccinated><novel><novel CoV><novel corona virus><novel coronavirus><predisposed to Coronavirus disease 2019><resistant><response><scRNA-seq><severe acute respiratory syndrome coronavirus 2 transmission><severe acute respiratory syndrome coronavirus 2 variant><severe acute respiratory syndrome coronavirus 2 variant forms><severe acute respiratory syndrome coronavirus 2 variant strains><severe acute respiratory syndrome-CoV><single cell RNA-seq><single cell RNAseq><single cell expression profiling><single cell transcriptomic profiling><single-cell RNA sequencing><susceptible to COVID-19><susceptible to Coronavirus disease 2019><susceptible to SARS-CoV-2><susceptible to Severe acute respiratory syndrome coronavirus 2><thymus derived lymphocyte><tool><transmitted COVID-19><transmitted SARS-CoV-2><transmitted coronavirus disease 2019><transmitted severe acute respiratory syndrome coronavirus 2><unvaccinated><vaccinate against COVID-19><vaccinate against SARS-CoV-2><vaccinate against coronavirus disease 2019><vaccinate against severe acute respiratory syndrome coronavirus 2><vaccination against COVID-19><vaccination against SARS-CoV-2><vaccination against Severe acute respiratory syndrome coronavirus 2><vaccination against coronavirus disease 2019><vaccine candidate><variants of concern><viral detection><virus detection><vulnerable to COVID-19><vulnerable to Coronavirus disease 2019><vulnerable to SARS-CoV-2><vulnerable to Severe acute respiratory syndrome coronavirus 2><β CoV><β coronavirus><βCoV>