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Principal Investigator: JERREL L YAKEL
Organization: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
Fiscal Year: 2024
Award: $1,719,113
Funding agency: National Institute of Environmental Health Sciences
The Viral Vector Core facility currently offers support for production, purification, titering, and validation of twelve types of viruses: Adenovirus, Adenoassociated virus (AAV), coronaviruses (229E, OC43, NL63, and MHV), Herpes Simplex Virus (HSV), Influenza A, lentiviruses including human immunodeficiency virus (HIV), Lymphocytic choriomeningitis virus (LCMV), Rabies-delta-G (SAD-B19-dG, dGL, SiR, and CVS-N2c), Retrovirus, Respiratory syncytial virus (RSV), Sindbis Virus, vesicular stomatitis virus (VSV) and Zika virus. The core utilizes multiple vector systems to safely produce recombinant, pseudotyped and wild type viruses.
This year, the Viral Vector Core offered services for propagation and transduction assays using Respiratory Syncytial Virus (RSV), SARS coronavirus and SARS coronavirus 2 spike pseudotyped virions and coronavirus strains 229E, OC43, NL63, and MHV. These virions were used in transduction assays to screen a multitude of anti-viral reagents. HIV-1 and SARS-CoV-2 pseudotyped virions were also utilized for structural studies of the virus spike.
In addition, the core facility developed transduction protocols for gene delivery to primary or immortalized cell lines and animal tissue. In the past year, the Viral Vector Core has packaged and validated 377 viral samples for gene delivery. These vectors have been utilized in research to express ion channels, transcription factors, biological indicators, hormone receptors, secreted protein expression and purification, gene knockdown by shRNA or genetic modification using CRISPR components. Viral vectors were also used to introduce genes into primary and immortalized cell lines, mouse and rat tissue, or cultured tissue. Other difficult to transfect cell lines, such as hematopoietic and mouse embryonic cells, were also successfully transduced using viral vectors. Lentiviral vectors facilitated immortalizing primary cell lines for toxicological studies and several viral strains were imported for use in immunological studies.
The Viral Vector Core supports research in the Division of Division of Translational Toxicology (DTT), Clinical Research Branch (CRB), Epigenetic and Stem Cell Biology Laboratory (ESCBL), Genome Integrity and Structural Biology Laboratory (GISBL), Immunity, Inflammation and Disease Laboratory (IIDL), Neurobiology Laboratory (NL), Signal Transduction Laboratory (STL), and Reproductive and Developmental Biology Laboratory (RDBL) at the NIEHS by providing highly effective means of gene delivery.
The core also provided viral products for laboratories in NCCIH, NHLBI, NIA, NIAID, NICHD, NIDDK, NINDS and NIMH via the Collaborative Research Exchange (CREx) program.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><2019-nCoV S protein><2019-nCoV spike glycoprotein><2019-nCoV spike protein><AIDS Virus><Accountability><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome Virus><Adeno-Associated Viruses><Adenoviridae><Adenoviruses><Assay><Basal Transcription Factor><Basal transcription factor genes><Bioassay><Biochemical><Biological><Biological Assay><Body Tissues><COVID-19 S protein><COVID-19 spike><COVID-19 spike glycoprotein><COVID-19 spike protein><COVID-19 virus><COVID19 virus><CRISPR><CRISPR/Cas system><Cell Body><Cell Communication and Signaling><Cell Line><Cell Signaling><CellLine><Cells><Clinical Research><Clinical Study><Clustered Regularly Interspaced Short Palindromic Repeats><CoV-2><CoV2><Common Rat Strains><Consultations><Core Facility><Coronaviridae><Coronavirus><Culturing, in vitro Vertebrate, Primary><Dependoparvovirus><Dependovirus><Development><Developmental Biology><Disease><Disorder><Ensure><Epigenetic><Epigenetic Change><Epigenetic Mechanism><Epigenetic Process><Gene Delivery><General Transcription Factor Gene><General Transcription Factors><Genes><Genetic><Guidelines><HCoV><HIV><HIV-1><HIV-I><HIV1><HSV><Hematopoietic><Herpes Simplex Virus><Herpes labialis Virus><Hormone Receptor><Human Immunodeficiency Virus Type 1><Human Immunodeficiency Viruses><Human immunodeficiency virus 1><Immunity><Immunochemical Immunologic><Immunologic><Immunological><Immunologically><Immunologics><In Vitro><Inflammation><Influenza A><Influenza A virus><Influenza Virus><Influenza Viruses Type A><Influenzavirus A><Integrase><Intracellular Communication and Signaling><Investigators><Ion Channel><Ionic Channels><LAV-HTLV-III><LCM Viruses><LCMV><Laboratories><Lentiviral Vector><Lentivirinae><Lentivirus><Lentivirus Vector><Lymphadenopathy-Associated Virus><Lymphocytic choriomeningitis virus><Membrane Channels><Mice><Mice Mammals><Mission><Modification><Murine><Mus><NCCAM><NCCIH><NHLBI><NIAID><NICHD><NIDDK><NIEHS><NIH><NIMH><NINDS><National Center for Complementary and Integrative Health><National Center for Complementary and Integrative Medicine><National Heart, Lung, and Blood Institute><National Institute of Allergy and Infectious Disease><National Institute of Child Health and Human Development><National Institute of Children's Health and Human Development><National Institute of Diabetes and Digestive and Kidney Diseases><National Institute of Environmental Health Sciences><National Institute of Mental Health><National Institute of Neurological Diseases and Stroke><National Institute of Neurological Disorders and Stroke><National Institutes of Health><Neurobiology><Orthomyxovirus Type A><Pathway interactions><Primary Cell Cultures><Principal Investigator><Production><Protein Secretion><Protocol><Protocols documentation><Rabies><Rat><Rats Mammals><Rattus><Reagent><Recombinants><Reproductive Biology><Research><Research Personnel><Research Support><Researchers><Respiratory syncytial virus><Retroviridae><Retroviruses><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CO-V2><SARS-COVID-2><SARS-CoV><SARS-CoV-1><SARS-CoV-2><SARS-CoV-2 S><SARS-CoV-2 S protein><SARS-CoV-2 spike><SARS-CoV-2 spike glycoprotein><SARS-CoV-2 spike protein><SARS-CoV2><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Safety><Sampling><Services><Severe Acute Respiratory Coronavirus><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute 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culture><transcription factor><validations><vector><viral gene delivery><zikav>