A full-length cDNA clone of SARS-CoV-2.

NIH Pandemic-Era Grants

Pandemic Era Grants

2021

↗ Live URL (may be dead)

Document text

Principal Investigator: ALEXANDER  PLETNEV
Organization: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Fiscal Year: 2021
Award: $16,970
Funding agency: National Institute of Allergy and Infectious Diseases

To achieve our goal, we generated a full-length infectious cDNA clone of SARS-CoV-2 coronavirus (isolate 2019-nCoV/USA_WA1/2020) and modified it for mouse model by introducing mouse-adapted mutations. In addition, using this cDNA we generated infectious clones for the three epidemiologically-significant variants of SARS-CoV-2: (1) UK variant (lineage B.1.1.7) was constructed by introducing 23 mutations into 2019-nCoV/USA_WA1/2020; (2) South African variant (lineage B.1.351) was constructed by introducing 21 mutations; (3) Brazilian/Manaus variant (lineage P.1) was constructed by introducing 24 mutations. To delineate the role of specific S-gene mutations in emergence of novel SARS-CoV-2 variants, we generated a panel of chimeric SARS-CoV-2 virus constructs. For that, mutations identified in the S gene of B.1.1.7, B.1.351, P.1 lineages were introduced into the genetic backbone of SARS-CoV-2 cDNA (isolate 2019-nCoV/USA_WA1/2020). Recovery and characterization of recombinant viruses in cell culture and/or in mouse model have been performed or are currently on-going in the laboratories of Dr. D. R. Smith (Naval Medical Research Center, Fort Frederick) and Dr. S. Best, (Rocky Mountain National Laboratories, NIAID).

Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><2019-nCoV variant><2019-nCoV variant forms><2019-nCoV variant strains><B.1.1.7><B.1.351><COVID-19><COVID-19 variant><COVID-19 variant forms><COVID-19 variant strains><COVID-19 virus><COVID19><COVID19 virus><CV-19><CV19><Cell Culture Techniques><CoV-2><CoV2><Complementary DNA><Coronaviridae><Coronavirus><Epidemiology><Gene Alteration><Gene Mutation><Genes><Genetic><Genetic Alteration><Genetic Change><Genetic defect><Goals><Laboratories><Length><Medical Research><Mice><Mice Mammals><Murine><Mus><Mutation><NIAID><National Institute of Allergy and Infectious Disease><Pathogenesis><R-Series Research Projects><R01 Mechanism><R01 Program><Recovery><Research Grants><Research Project Grants><Research Projects><Role><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CoV><SARS-CoV-2><SARS-CoV-2 B.1.1.7><SARS-CoV-2 B.1.351><SARS-CoV-2 pathogenesis><SARS-CoV-2 variant><SARS-CoV-2 variant forms><SARS-CoV-2 variant strains><SARS-CoV2><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><South Africa variant><South African variant><Spinal Column><Spine><System><Therapeutic><U.K. variant><UK variant><United Kingdom variant><Variant><Variation><Vertebral column><Virus><Wuhan coronavirus><animal model development><backbone><cDNA><cell culture><corona virus><corona virus disease 2019><coronavirus disease 2019><coronavirus disease 2019 variant><coronavirus disease 2019 variant forms><coronavirus disease 2019 variant strains><coronavirus disease 2019 virus><develop a vaccine><development of a vaccine><epidemiologic><epidemiological><genome mutation><hCoV19><human disease><mouse model><murine model><nCoV2><novel><recombinant virus><reverse genetics><severe acute respiratory syndrome coronavirus 2 B.1.1.7><severe acute respiratory syndrome coronavirus 2 B.1.351><severe acute respiratory syndrome coronavirus 2 pathogenesis><severe acute respiratory syndrome coronavirus 2 variant><severe acute respiratory syndrome coronavirus 2 variant forms><severe acute respiratory syndrome coronavirus 2 variant strains><severe acute respiratory syndrome-CoV><social role><vaccine candidate><vaccine development><vaccine formulation>