Document text
Principal Investigator: Xiang Zhang
Organization: UNIVERSITY OF LOUISVILLE
Fiscal Year: 2020
Award: $459,749
Funding agency: National Institute on Alcohol Abuse and Alcoholism
Abstract:
The primary function of Omics Core is to provide members of the ULARC with the expertise, resources and
facilities needed for metabolomic, proteomic and bioinformatic analyses of biological samples collected to
study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. OMICS Core
personnel will assist with the design of sample collection protocols, will drive the development and the
execution of novel and robust chromatographic methods for sample separation and mass spectrometric
methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater
understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction.
The core will ensure uniformity and standardization in sample preparation, method development, instrument
performance, data collection, data reporting and data sharing. The Core endeavors to contribute to the overall
success and utility of the ULARC and to enhance the infrastructure and the technical and academic capabilities
of the institution as a whole. In addition to its function as a service core, we will also promote the education and
training of the ULARC members with regards to state-of–the-art Omics and Bioinformatics methods; with
particular attention to the ULARC junior investigators.
Terms: <1,2,3-Tributyrylglycerol><2-Propenal><2-dimensional><Acids><Acraldehyde><Acrolein><Acrylaldehyde><Acrylic Aldehyde><Affinity><Alcohol Chemical Class><Alcoholic Liver Diseases><Alcohols><Allyl Aldehyde><Animals><Attention><Attenuated><Automobile Driving><Bio-Informatics><Bioinformatics><Biological><Biotin><Blood><Blood Reticuloendothelial System><Blood Sample><Blood specimen><Body Tissues><Body Weight><Butanoic Acid 1,2,3-Propanetriyl Ester><Cell Body><Cell Line><Cell-Extracellular Matrix><CellLine><Cells><Chronic><Co-Stimulator><Complex Mixtures><Costimulator><Curcumin><Data><Data Collection><Data Reporting><Data Set><Dataset><Development><Diferuloylmethane><Drug Interactions><ECM><Education and Training><Embryo><Embryonic><Endotoxemia><Ensure><Environment><Epidermal Thymocyte Activating Factor><Epigenetic><Epigenetic Change><Epigenetic Mechanism><Epigenetic Process><Ethylene Aldehyde><Exposure to><Extracellular Matrix><Feces><Fibroblasts><Gas Chromatography><Ginger><Glyceryl tributyrate><Goals><Grapefruit><HDAC><HDAC Proteins><HPLC><Hepatic><Hepatic Disorder><High Performance Liquid Chromatography><High Pressure Liquid Chromatography><High Speed Liquid Chromatography><Histone Acetylation><Histone Deacetylase><Histones><Human Resources><IL-2><IL2 Protein><Inflammation><Infrastructure><Injury><Injury to Liver><Institution><Interleukin 2><Interleukin 2 Precursor><Interleukin II><Interleukin-2><Interleukine 2><Interleukine 2 Precursor><Interleukine II><Intestinal><Intestines><Investigators><L rhamnosus><L-Lysine><L. rhamnosus><LC/MS><Lactobacillus casei rhamnosus><Lactobacillus rhamnosus><Liquid substance><Liver><Liver Dysfunction><Liver diseases><Lung><Lung Respiratory System><Lymphocyte Mitogenic Factor><Lysine><Manpower><Mass Photometry/Spectrum Analysis><Mass Spectrometry><Mass Spectroscopy><Mass Spectrum><Mass Spectrum Analyses><Mass Spectrum Analysis><Measures><Mediating><Metabolic><Methodology><Methods><Mice><Mice Mammals><Mitogenic Factor><Molecular><Molecular Evolution><Murine><Mus><Neural Crest Cell><Oral><Pathogenicity><Pattern><Peptides><Performance><Post-Translational Modification Protein/Amino Acid Biochemistry><Post-Translational Modifications><Post-Translational Protein Modification><Post-Translational Protein Processing><Posttranslational Modifications><Posttranslational Protein Processing><Preparation><Protein Analysis><Protein Fragment><Protein Modification><Proteins><Proteomics><Protocol><Protocols documentation><Research><Research Personnel><Research Resources><Researchers><Resolution><Resources><Role><Sampling><Science><Services><Standardization><Strains Cell Lines><Sulfaforaphane><Sulforaphane><T cell growth factor><T-Cell Growth Factor><T-Cell Stimulating Factor><Techniques><Technology><Thymocyte Stimulating Factor><Tissue Sample><Tissues><Toronja><Training and Education><Transcription Regulation><Transcriptional Control><Transcriptional Regulation><Turmeric Yellow><Unsaturated Fats><Urine><Urine Urinary System><Vitamin H><Zingiber officinale><acryaldehyde><alcohol effect><alcohol induced hepatic injury><alcohol induced liver disorder><alcohol induced liver injury><alcohol-induced hepatic dysfunction><alcohol-induced liver disease><alcohol-induced liver dysfunction><alcohol-mediated liver dysfunction><alcohol-mediated liver injury><alcoholic liver injury><bowel><coenzyme R><cultured cell line><data representation><data sharing><design><designing><developmental><driving><ethanol effect><ethanol induced hepatic injury><ethanol induced liver disorder><ethanol induced liver injury><ethanol-induced hepatic dysfunction><ethanol-induced liver disease><ethanol-induced liver dysfunction><ethanol-mediated liver dysfunction><ethanol-mediated liver injury><exosome><fluid><hepatic body system><hepatic damage><hepatic disease><hepatic injury><hepatic organ system><hepatopathy><histone methylation><histone modification><injuries><injury to organs><instrument><liquid><liquid chromatography mass spectrometry><liver damage><liver disorder><liver injury><long chain fatty acid><male><member><metabolism measurement><metabolomics><metabonomics><method development><methylsulfoxybutylisothiocyanate><multiple reaction monitoring><nano particle><nano-sized particle><nanoparticle><nanosized particle><novel><nutrition><organ injury><personnel><phospho-proteomics><phosphoproteomics><programs><pulmonary><response><sample collection><saturated dietary fat><saturated dietary lipid><saturated fat><saturated lipid><social role><specimen collection><stool><success><sulforafan><sulforophane><time of flight mass spectrometry><toxicant><tributyrin><tributyrylglycerol><two-dimensional><unsaturated dietary fat><unsaturated dietary lipid><unsaturated lipid><vapor phase chromatography>