Development of technologies for evaluation of antibody response following Coronavirus Infection vs Vaccination to identify immune correlates of protection
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Organization: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES Fiscal Year: 2022 Award: $175,000 Funding agency: National Institute of Allergy and Infectious Diseases The emergence of the Wuhan new coronavirus (nCoV) initiated efforts to rapidly develop effective medical countermeasures including vaccines and therapeutics against this severe disease. However, there is limited knowledge on polyclonal antibody responses generated following vaccination or nCoV infection in animals or humans. It is important to identify and understand immune markers that are reasonably likely to predict clinical benefit and that can facilitate evaluation of vaccine and therapeutic candidates, including: I. Do different vaccine platforms elicit similar or different antibody epitope repertoires, antibody isotypes, antibody affinity and durability in human’s vs animals? II. What are the correlates of protection against nCoV: Total antibody binding to specific antigenic regions within spike protein? antibody affinity? antibody isotype? antibody epitope diversity? III. Is the quantity, quality (epitopes and affinity) and durability of antibodies generated by nCoV- vaccination is similar or different than those of antibodies found in post-infection (in acute and convalescent) sera? To address these gaps, we will develop advanced technologies, including whole genome phage display library (GFPDL) and SPR for the new Coronavirus (nCoV) for in-depth analysis of immune responses following vaccination and nCoV infection in animal models or humans. Terms: <7S Gamma Globulin><Acute><Address><Affinity><Animal Model><Animal Models and Related Studies><Animals><Antibodies><Antibody Affinity><Antibody Diversity><Antibody Response><Antigenic Determinants><Binding><Binding Determinants><Blood Plasma><CoV emergence><Coronaviridae Infections><Coronavirus Infections><Disease><Disorder><Epitopes><Evaluation><Genome><Human><IgG><Immune><Immune Markers><Immune response><Immunes><Immunoglobulin G><Immunologic Markers><Immunological response><Infection><Kinetics><Knowledge><Libraries><Modern Man><Molecular Interaction><Phage Display><Plasma><Plasma Serum><Proteins><Regimen><Reticuloendothelial System, Serum, Plasma><Role><Sampling><Technology><Therapeutic><Vaccination><Vaccines><antigen antibody affinity><clinical predictors><corona virus emergence><coronavirus emergence><coronavirus vaccination><emergent CoV><emergent corona virus><emergent coronavirus><emerging CoV><emerging corona virus><emerging coronavirus><entire genome><evaluate vaccines><full genome><host response><immune system response><immune-based biomarkers><immunological biomarkers><immunological markers><immunoresponse><medical countermeasure><model of animal><model organism><nCoV><new CoV><new corona virus><new coronavirus><novel CoV><novel corona virus><novel coronavirus><polyclonal antibody><social role><tech development><technology development><therapeutic candidate><vaccination against coronavirus><vaccine candidate><vaccine evaluation><vaccine platform><vaccine screening><vaccine testing><whole genome>