Development of Safe and Rapid Reporter Viruses for Studying Pathogenic and Pandemic Viruses

Document text

Principal Investigator: Benjamin J Doranz
Organization: INTEGRAL MOLECULAR
Fiscal Year: 2024
Award: $288,515
Funding agency: National Institute of Allergy and Infectious Diseases

ABSTRACT
Neutralizing titer is a demonstrated correlate of protection across most viral pathogens. The traditional assay to
assess neutralization titer is the plaque reduction neutralization test (PRNT), which is generally regarded as
slow, subjective, and variable. To conduct large scale clinical vaccine trials, thousands of serum samples from
vaccinated individuals are typically assessed in neutralization assays, a scale that is difficult to achieve using
live virus in high containment facilities. Optical reporters such as GFP and luciferase encoded in ‘reporter
viruses’ are an alternative to PRNT and are more streamlined, safe, and scalable than PRNT assays but still
require a week of experimentation. Here we propose to develop a Rapid Reporter Virus (RRV) to assess virus
infection and neutralization rapidly and safely. The development of this novel assay is in direct response to our
customers’ requests for a better neutralization readout.

Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><Abbreviations><Antibodies><Assay><Authorization><Authorization documentation><Bioassay><Biological Assay><Blood Serum><COVID-19 virus><COVID19 virus><Catalogs><Cell Body><Cell Communication and Signaling><Cell Culture Techniques><Cell Signaling><Cells><Classification><Clinical><CoV-2><CoV2><Containment><Cryofixation><Cryopreservation><Cryopreserved Cell><Cultured Cells><Development><Disease><Disorder><Emergencies><Emergency Situation><Enzyme Gene><Enzymes><Family><Freezing><Future><H5N1><H5N1 virus><Health><Hour><Human Resources><Industry><Infection><Influenza A H5N1><Influenza A Virus, H5N1 Subtype><Intracellular Communication and Signaling><Investigators><Laboratories><Lassa fever virus><Lassa virus><Luciferase Immunologic><Luciferases><Manpower><Measurement><Measures><Molecular><NIAID><NIH><National Institute of Allergy and Infectious Disease><National Institutes of Health><Neutralization Tests><North America><Optical reporter><Optics><Permission><Preparedness><Procedures><Production><Proteins><Public Health><Publishing><Quarantine><Readiness><Reagent><Recovery><Reporter><Reporting><Research Personnel><Researchers><Risk><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV2><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Serum><Services><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Signal Transduction><Signal Transduction Systems><Signaling><System><Systematics><Technology><Testing><Time><United States National Institutes of Health><VSV><Vaccination><Vaccine Clinical Trial><Vaccinee><Vaccines><Variant><Variation><Vesicular Stomatitis Virus><Vesicular stomatitis Indiana virus><Viral><Viral Diseases><Virion><Virus><Virus Diseases><Virus Particle><Work><Wuhan coronavirus><antibody neutralization test><avian influenza H5N1><avian influenza H5N1 virus><biological signal transduction><catalog><cell culture><cell cultures><cell preparation><cold preservation><cold storage><commercialization><coronavirus disease 2019 virus><coronavirus disease-19 virus><developmental><experience><experiment><experimental research><experimental study><experiments><future pandemic><hCoV19><improved><isolation/quarantine><nCoV2><next pandemic><novel><occupational health/safety><optical><pandemic><pandemic concern><pandemic containment><pandemic control><pandemic disease><pandemic disease preparedness><pandemic mitigation><pandemic planning><pandemic potential><pandemic preparedness><pandemic readiness><pandemic response><pandemic risk><pandemic threat><pandemic virus><pathogen><pathogenic virus><personnel><prototype><public health emergency><response><timeline><vaccinated individual><vaccinated participant><vaccinated patient><vaccinated person><vaccinated subject><viral infection><viral pathogen><virus infection><virus pathogen><virus-induced disease>