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Principal Investigator: David E Verhoeven
Organization: IOWA STATE UNIVERSITY
Fiscal Year: 2024
Award: $225,370
Funding agency: National Institute of Allergy and Infectious Diseases
PROJECT SUMMARY / ABSTRACT
Highly pathogenic and low pathogenic strains of avian influenza continue to circulate and infect birds in Asia with occa-
sional spread into the North American flyway. These necessitate the culling of any poultry farms that test positive for
these strains. Furthermore, through continued circulation, humans remain under constant threat that one of these strains
will reassort and infect humans. While the current strategy for combating these strains is surveillance and biosecurity,
these strategies clearly do not work in many parts of the world, as they are hot zones for these viruses. As an alternative,
vaccinations for poultry and waterfowl would certainly be a boon for human health as they would restrict the number of
influenza strains in these flu reservoirs. Here, we seek to use mRNA vaccines that code for a universal flu Hemagglutinin
antigen we have developed. In tandem with polyanhydride delivery vehicles that impart thermostability to mRNA and
serve as antigen depots, we will vaccinate chicken eggs to determine efficacy against highly pathogenic and low patho-
genic avian influenza strains. We will also develop transgenic mealworms to use as booster or as primary vaccines for
poultry, but eventually waterfowl, for future use as flu vaccine bait stations.
Terms: <1918 H1N1 pandemic><1918 flu pandemic><1918 influenza pandemic><1918 pandemic><Adjuvant><Adverse effects><American><Animals><Antigens><Asia><Asian><Attenuated><Attenuated Vaccines><Aves><Avian><Avian Influenza><Bird Flu><Birds><Cessation of life><Chickens><Circulation><Code><Coding System><Cold Chains><Containment><DNA><Death><Deoxyribonucleic Acid><Disease Outbreaks><Domestic Fowls><Domestic Horse><Equine><Equine Species><Equus caballus><Equus przewalskii><Exposure to><Farm><Ferrets><Fowl Pest><Fowl Plague><Future><Gallus domesticus><Gallus gallus><Gallus gallus domesticus><Gestation><Grippe><H1N1><H1N1 Virus><H3N2><H3N2 Virus><H3N8><H3N8 Virus><H5N1><H5N1 virus><Health><Hemagglutinin><Horses><Human><Immunity><Implant><In Vitro><Indiana><Industry><Infection><Influenza><Influenza A H5N1><Influenza A Virus, H1N1 Subtype><Influenza A Virus, H3N2 Subtype><Influenza A Virus, H3N8 Subtype><Influenza A Virus, H5N1 Subtype><Influenza HA><Influenza Hemagglutinin><Influenza Vaccines><Injections><Insecta><Insects><Insects Invertebrates><Iowa><Licensing><Lipids><Literature><Live-attenuated Vaccine><Mammalia><Mammals><Mealworm><Meleagris gallopavo><Messenger RNA><Mice><Mice Mammals><Midwest><Midwest U.S.><Midwest US><Midwestern United States><Modern Man><Molecular Configuration><Molecular Conformation><Molecular Stereochemistry><Mucosa><Mucosal Tissue><Mucous Membrane><Murine><Mus><Mutate><Newspapers><Oral><Outbreaks><Owls><Pathogenicity><Pattern><Persons><Polyanhydrides><Population><Poultry><Pregnancy><Prevention><Proteins><RNA immunization><RNA vaccination><RNA vaccine><RNA-based vaccine><Receptor Protein><Rod><Route><Sales><Sea><Spanish flu pandemic><Spanish influenza pandemic><Speed><Strigiformes><Technology><Tenebrio><Tennessee><Testing><Transcript><Transgenic Organisms><Turkey><US Department of Agriculture><USDA><United States Department of Agriculture><Vaccinated><Vaccination><Vaccines><Viral><Viral Diseases><Virus><Virus Diseases><anti-flu><anti-influenza><antiflu><attenuate><attenuates><avian flu><avian influenza H5N1><avian influenza H5N1 virus><avian strains of influenza><biosecurity><chicken egg><cold temperature><conformation><conformational><conformational state><conformationally><conformations><cost><cross immunity><cross protection><deliver vaccines><delivery vector><delivery vehicle><design><designing><determine efficacy><efficacy analysis><efficacy assessment><efficacy determination><efficacy evaluation><efficacy examination><efficacy study><egg><evaluate efficacy><evaluate vaccines><examine efficacy><feeding><flu><flu HA><flu hemagglutinin><flu serotype><flu strain><flu subtype><flu vaccine><flu viral strain><flu virus strain><flu virus vaccine><future pandemic><genetically modified insect><hatching><immunogen><influenza in birds><influenza serotype><influenza strain><influenza subtype><influenza viral HA><influenza viral hemagglutinin><influenza viral strain><influenza virus HA><influenza virus hemagglutinin><influenza virus strain><influenza virus vaccine><live vaccine><live vaccines><low temperature><mRNA><mRNA immunization><mRNA vaccination><mRNA vaccine><mRNA-based vaccine><manufacture><neutralizing antibody><news><next pandemic><novel><pan influenza vaccine><pan influenza viral vaccine><pan influenza virus vaccine><pathogen><prevent><preventing><receptor><thermolability><thermostability><transgenic><transgenic insect><universal flu vaccine><universal influenza vaccine><universal influenza virus vaccine><universal vaccine><universal vaccine against flu><universal vaccine against influenza><vaccine against flu><vaccine against influenza><vaccine candidate><vaccine delivery><vaccine evaluation><vaccine screening><vaccine testing><viral infection><virus infection><virus-induced disease><wild bird>