UTHSC Regional Biocontainment Laboratory: Upgrade of Facility and Building System

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Principal Investigator: Colleen B Jonsson
Organization: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
Fiscal Year: 2021
Award: $3,211,742
Funding agency: National Institute of Allergy and Infectious Diseases

Project Summary
The University of Tennessee Health Sciences Center (UTHSC) proposes to upgrade facilities and broaden
research services in the existing UTHSC Regional Biocontainment Laboratory (RBL) located in Memphis, TN to
support internal and external investigators in basic and translational research with emerging pathogens and
select agents utilizing small animal. In Aim 1, upgrading the hardware and building automation system software
that has been in place since 2009 is our top priority with this funding opportunity and will allow the UTHSC
RBL to operate continuously well into the future. In Aim 2, we request support to expand existing infrastructure
in order to use our current facility to its design capacity with additional caging and racks. Importantly, a major
component of our plan is to upgrade our vivarium to house the Provantis Instem to provide support for preclinical
services. In Aim 3 and 4, we request equipment for whole animal imaging and immunological analyses to
strengthen our ability to meet the research service demands in terms of endpoints available to users; both
internally and externally. In summary, this proposal seeks to: (1) upgrade and replace outdated or obsolete
components of the building management system; (2) expand infrastructure for small animals other than mice,
(3) upgrade current animal imaging capabilities and dedicate one animal biosafety level 3 (ABSL3) laboratory
for the IVIS/CT imaging system, (4) enable preclinical services with a dedicate ABSL-3 area for Good Laboratory
Practice (GLP) monitoring; and (5) equipment to enable immunological analyses currently not available (elispot)
and replace an obsolete flow cytometry equipment with a spectral flow cytometer.

Terms: <Air><Air Movements><Animals><Area><Assay><Automation><Basic Research><Basic Science><Bioassay><Biologic Assays><Biological Assay><Bioluminescence><Biomedical Research><CAT scan><COVID-19><COVID19><CT X Ray><CT Xray><CT imaging><CT scan><CV-19><CV19><Cell Body><Cells><Chills><Clinical Research><Clinical Study><Complement><Complement Proteins><Computed Tomography><Coupled><Cricetinae><Data><Development><Development and Research><Disease><Disorder><Dysfunction><Equipment><Failure><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Microfluorimetry><Flow Microfluorometry><Fluorescence><Fostering><Functional disorder><Funding Opportunities><Future><Glycols><Goals><HEPA Filter><HEPA Filtration><HEPA cleaner><Hamsters><Hamsters Mammals><Health Sciences><Heating><Hydrogen Oxide><Illumination><Image><Immune response><Immunochemical Immunologic><Immunologic><Immunological><Immunological response><Immunologically><Immunologics><Infection><Infectious Agent><Infrastructure><Investigators><Laboratories><Length of Life><Lighting><Logic><Longevity><Maintenance><Manufacturer><Manufacturer Name><Methods><Mice><Mice Mammals><Mission><Modernization><Monitor><Murine><Mus><Optics><Physiopathology><Plants><Plaque Assay><R & D><R&D><Reaction Time><Recovery><Research><Research Personnel><Researchers><Response RT><Response Time><Services><Structure><System><Tennessee><Therapeutic><Time><Tomodensitometry><Translational Research><Translational Science><Tube><Universities><Update><Viral><Water><X-Ray CAT Scan><X-Ray Computed Tomography><X-Ray Computerized Tomography><Xray CAT scan><Xray Computed Tomography><Xray computerized tomography><air flow><airflow><animal imaging><biodefense><catscan><computed axial tomography><computer tomography><computerized axial tomography><computerized tomography><confocal imaging><corona virus disease 2019><coronavirus disease 2019><design><designing><develop a vaccine><development of a vaccine><developmental><diol><emerging pathogen><exhaust><flow cytophotometry><good laboratory practice><heat exchanger><high efficiency particulate air cleaner><high efficiency particulate air filter><high-efficiency particulate air purifier><host response><image-based method><imaging><imaging capabilities><imaging method><imaging modality><imaging system><immune system response><immunoresponse><infectious disease treatment><infectious organism><life span><lifespan><new pathogen><novel pathogen><operation><optical><pathogen><pathophysiology><pre-clinical><preclinical><pressure><psychomotor reaction time><research and development><software systems><therapeutic agent development><therapeutic development><translation research><trend><vaccine development><vaccine formulation><whole animal imaging>