Molecular imaging of viral infections

NIH Pandemic-Era Grants

Pandemic Era Grants

2024

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Principal Investigator: Dima A Hammoud
Organization: CLINICAL CENTER
Fiscal Year: 2024
Funding agency: NIH Clinical Center

Progress:
•	HIV project: We have optimized our model of Gp120 expression in a tumor model and succeeflly imaged the animals using 18F-lableed nanobodies. We also developed, in collaboration with NHLBI investigators, a method for site specific labeling of the nanobodies. We found that site-specifically labeled J3 is the best nanobody.  We wrote a manuscript which is currently being revised for publication.
We are now testing the same nanobody in animal model of varying levels of gp120 expression.
•	Ebola and other high consequence viral infection: We are collaborating with the Integrated research facility on multiple studies evaluating Nipah, Lassa, Marburg, Covid-19 and Ebola infections. 
•	Sars-Cov-2 project: We have finished our experiments and are currently writing the manuscript.

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2019><coronavirus disease 2019 S protein><coronavirus disease 2019 infection><coronavirus disease 2019 spike glycoprotein><coronavirus disease 2019 spike protein><coronavirus disease 2019 virus><coronavirus disease-19><coronavirus disease-19 virus><coronavirus infectious disease-19><developmental><experiment><experimental research><experimental study><experiments><gp120><gp120 ENV Glycoprotein><gp120(HIV)><hCoV19><image-based method><imaging><imaging in vivo><imaging method><imaging modality><improved><in vivo><in vivo imaging><infected with COVID-19><infected with COVID19><infected with SARS-CoV-2><infected with SARS-CoV2><infected with coronavirus disease 2019><infected with severe acute respiratory syndrome coronavirus 2><injured CNS><intervention therapy><lung injury><lung pathology><microPET/CT><model of animal><molecular imaging><molecule imaging><mouse model><murine model><nCoV2><nano label><nanobodies><nanobody><nanolabel><natural antibodies><non-contrast CT><non-invasive imaging><noncontrast CT><noncontrast computed tomography><noninvasive imaging><pathophysiology><positron emission tomographic (PET) imaging><positron emission tomographic imaging><positron emitting tomography><post treatment><protection pathway><protective pathway><pulmonary><pulmonary damage><pulmonary injury><pulmonary tissue damage><pulmonary tissue injury><radiolabeling><radiologically labeled><receptor><research facility><response><sdAb><single domain antibodies><social role><spike proteins on SARS-CoV-2><tumor><viral infection><viral multiplication><viral replication><virus infection><virus multiplication><virus-induced disease><wildtype mouse>