Antagonism of Coronavirus Spike Proteins by Cellular Host Factors

NIH Pandemic-Era Grants

Pandemic Era Grants

2020

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Principal Investigator: eric  freed
Organization: DIVISION OF BASIC SCIENCES - NCI
Fiscal Year: 2020
Award: $232,377
Funding agency: National Cancer Institute

In a structural and functional analysis of the SARS-CoV-2 spike protein that we recently started, we have obtained preliminary data indicating that that MARCH8, but not a catalytically inactive MARCH8 mutant, targets the spike proteins of SARS-CoV, MERS-CoV, and SARS-CoV-2 for degradation. We also determined that human airway epithelial cells express MARCH8 upon treatment with interferon. The work on MARCH protein-mediated antagonism may reveal new opportunities for therapeutic intervention with spike protein trafficking and incorporation into virus particles.

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