Document text
Principal Investigator: Andrew Leon Kau
Organization: CHILDREN'S HOSP OF PHILADELPHIA
Fiscal Year: 2024
Award: $669,712
Funding agency: Eunice Kennedy Shriver National Institute of Child Health and Human Development
PROJECT SUMMARY
The gut microbiome has a profound effect on human health by influencing the development of the immune
system, producing bioactive metabolites and directly aiding in the processing of food. Changes in the
composition and metabolism of the gut microbial community has been associated with a wide array of human
diseases, including malnutrition, auto-immunity, and even neurological function. A central aspiration of gut
microbiome research is to identify individuals with abnormalities in gut microbial composition in order to intervene
therapeutically. However, a major limitation is that it is logistically challenging to evaluate gut microbial
composition in a patient-care setting. The studies in this proposal will advance a novel strategy to overcome this
roadblock. Based on powerful preliminary studies in germ-free mice and in humans, our central hypothesis is
that the volatile composition of breath offers a dynamic representation of the composition and function of the gut
microbiota. We will test this hypothesis by evaluating the association between breath, gut bacterial species, and
microbial metabolic functions (Aim 1). In addition, we will use two unique clinical cohorts of infants to determine
how the breath volatile profile changes dynamically with changes in gut microbial community composition (Aim
2). Together, our studies will not only advance our fundamental understanding of the spectrum of breath volatiles
that arise from microbial metabolism, but will also be a critical first step in determining how “breathalyzer”
diagnostics of gut health can be applied clinically.
Terms: <Admission><Admission activity><Affect><Age><Age Months><Anabolism><Animal Husbandries><Animal Husbandry><Antibiotic Agents><Antibiotic Drugs><Antibiotic Therapy><Antibiotic Treatment><Antibiotics><Autoimmune Status><Autoimmunity><B fragilis><B. fragilis><Bacteria><Bacteroides fragilis><Biological><Biological Markers><Bionomics><Birth><Breath Tests><COVID-19><CV-19><Cancers><Cardiac Diseases><Cardiac Disorders><Catabolism><Catalogs><Characteristics><Childhood><Chronic lung disease><Clinical><Clinical Research><Clinical Study><Cohort Studies><Commerce><Concurrent Studies><Coronavirus Infectious Disease 2019><Data><Development><Diagnosis><Diagnostic><Diagnostic tests><Diet><Disease><Disorder><Disulfides><Drugs><Ecology><Enrollment><Ensure><Environmental Exposure><Evaluation><Exhalation><Exhaling><Fever><Food Processing><Functional Metagenomics><GI microbiome><GI microbiota><Gastrointestinal microbiota><Gene Transcription><Genetic Transcription><Genetic study><Germ-Free><Gnotobiotic><Gnotobiotics><Goals><Health><Heart Diseases><Human><Immune system><Immunity><In Vitro><Individual><Infant><Intermediary Metabolism><Investigation><Knowledge><Link><Lyase><Lyase Gene><Malignant Neoplasms><Malignant Tumor><Malnutrition><Measurement><Measures><Medical><Medication><Metabolic><Metabolic Pathway><Metabolic Processes><Metabolism><Metagenomics><Methionine><Methodology><Mice><Mice Mammals><Microbiomics><Miscellaneous Antibiotic><Modern Man><Molecular><Murine><Mus><Nervous System Physiology><Neurologic function><Neurological function><Nutritional Deficiency><Parturition><Patient Care><Patient Care Delivery><Pattern><Pharmaceutical Preparations><Population><Prospective cohort><Public Health><Pyrexia><RNA Expression><Research><Research Specimen><Respiratory Expiration><Shapes><Specimen><System><Technology><Testing><Therapeutic><Time><Transcription><Undernutrition><Volatilization><Work><absorption><ages><aspirate><bacteria metabolism><bacterial disease treatment><bacterial infectious disease treatment><bacterial metabolism><bio-markers><biologic><biologic marker><biomarker><biosynthesis><breath analysis><breath composition><candidate validation><care for patients><care of patients><caring for patients><catalog><chronic pulmonary disease><clinical diagnostics><cohort><coronavirus disease 2019><coronavirus disease-19><coronavirus infectious disease-19><developmental><dietary deficiency><diets><digestive tract microbiome><drug/agent><dysbacteriosis><dysbiosis><dysbiotic><enroll><enteric microbial community><enteric microbiome><enteric microbiota><experience><febrile><febris><fecal microbial community><fecal microbiota><fecal sample><gastrointestinal microbial flora><gastrointestinal microbiome><gut commensal><gut community><gut flora><gut health><gut microbe community><gut microbes><gut microbial community><gut microbial composition><gut microbial consortia><gut microbial species><gut microbiome><gut microbiota><gut microbiotic><gut microflora><gut-associated microbiome><heart disorder><human disease><improved><infancy><infantile><innovate><innovation><innovative><intestinal biome><intestinal flora><intestinal microbes><intestinal microbiome><intestinal microbiota><intestinal microflora><intestinal tract microflora><malignancy><malnourished><medical diagnostic><member><metagenome sequencing><metagenomic sequencing><metatranscriptomics><microbial><microbial imbalance><microbiome research><microbiome science><microbiome studies><mouse model><murine model><neoplasm/cancer><nervous system function><new approaches><novel approaches><novel strategies><novel strategy><nutrition deficiency><nutrition deficiency disorder><nutritional deficiency disorder><pediatric><point of care testing><response><stool sample><stool specimen><success><volatile organic chemical><volatile organic compound>