Document text
Principal Investigator: ROBERT J LINHARDT
Organization: RENSSELAER POLYTECHNIC INSTITUTE
Fiscal Year: 2020
Award: $343,946
Funding agency: NIH Office of the Director
Summary
Title: Biacore T200 Surface Plasmon Resonance Spectroscopy
Surface plasmon resonance (SPR) spectroscopy is a powerful instrument to measure
biomolecular interactions in real-time and a label free environment. SPR has been
applied to characterize the binding events with samples ranging from proteins, nucleic
acids, carbohydrates, small molecules to complex mixtures, lipid vesicles, viruses, bacteria,
and eukaryotic cells. This project is a Shared Instrumentation Grant Program (S10)
application entitled “Biacore T200 Surface Plasmon Resonance Spectroscopy”. The Biacore
T200 is a versatile, label-free system for detailed studies of biomolecular interactions
delivering high quality kinetic, affinity and thermodynamic interaction data in real time with
exceptional sensitivity. The system will be very useful in fundamental biological studies,
health/clinic science research, drug discovery, environmental and biopharmaceutical
process monitoring for large groups of researchers in our Center for Biotechnology &
Interdisciplinary Studies (CBIS). A Biacore 3000 instrument currently housed in CBIS has
reached the end of its useful life with the discontinuation of this instrument model. This
instrument was used in over 100 research studies examining protein-protein, protein-
glycan, glycan-glycan, protein-lipid, protein-DNA, DNA-DNA, protein-nanoparticle
interactions published by CBIS faculty from 2004-2019. Many CBIS research groups will
use the more sensitive and higher throughput Biacore T200 SPR to study the molecular
interactions with important physiological significance in signaling and developmental biology
and pathophysiological significance, including ones related to cancer, kidney disease,
infectious disease, and Alzheimer’s disease. The data from SPR analysis will provide a
deeper understanding of many of the biochemical pathways and networks.
Terms: <AD dementia><Affinity><Alzheimer><Alzheimer Type Dementia><Alzheimer disease><Alzheimer sclerosis><Alzheimer syndrome><Alzheimer's><Alzheimer's Disease><Alzheimer's disease dementia><Alzheimers Dementia><Alzheimers disease><B220><Bacteria><Binding><Biochemical Pathway><Biologic Products><Biological><Biological Agent><Biological Products><Biotech><Biotechnology><CD45><Cancers><Carbohydrates><Cell Communication and Signaling><Cell Signaling><Clinic><Communicable Diseases><Complex Mixtures><DNA><Data><Deoxyribonucleic Acid><Developmental Biology><Environment><Eukaryotic Cell><Event><Faculty><GP180><Glycans><Grant><Health><Infectious Disease Pathway><Infectious Diseases><Infectious Disorder><Interdisciplinary Research><Interdisciplinary Study><Intracellular Communication and Signaling><Investigators><Kidney Diseases><Kinetics><LY5><Label><Life><Lipids><Malignant Neoplasms><Malignant Tumor><Measures><Metabolic Networks><Modeling><Molecular Interaction><Monitor><Multidisciplinary Collaboration><Multidisciplinary Research><Nephropathy><Nucleic Acids><PTPRC><PTPRC gene><Physiologic><Physiological><Polysaccharides><Primary Senile Degenerative Dementia><Process><Proteins><Publishing><Renal Disease><Research><Research Personnel><Researchers><Sampling><Science><Signal Transduction><Signal Transduction Systems><Signaling><Spectroscopy><Spectrum Analyses><Spectrum Analysis><Surface Plasmon Resonance><System><T200><Thermodynamic><Thermodynamics><Time><Vesicle><Virus><biological signal transduction><biopharmaceutical><biotherapeutic agent><dementia of the Alzheimer type><drug discovery><instrument><instrumentation><kidney disorder><malignancy><nano particle><nano-sized particle><nanoparticle><nanosized particle><neoplasm/cancer><primary degenerative dementia><programs><renal disorder><research study><senile dementia of the Alzheimer type><small molecule>