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Principal Investigator: Patrick Kellish
Organization: UNIVERSITY OF FLORIDA
Fiscal Year: 2023
Award: $78,287
Funding agency: National Cancer Institute
FLOW CYTOMETRY AND CONFOCAL MICROSCOPY SHARED RESOURCE (CYT-SR): ABSTRACT
CYT-SR provides University of Florida Health Cancer Center (UFHCC) members with cost-effective, state-of-
the-art flow cytometry and modern confocal microscopy expertise, instrumentation, and services to support their
research activities. The CYT-SR is an institutionally-managed shared resource (SR) that is supported by UFHCC
through a partnership with the UF Interdisciplinary Center for Biotechnology Research (ICBR). It is co-located
within the Cancer & Genetics Research Complex and McKnight Brain Institute, providing convenient access for
UFHCC members. The CYT-SR has 4 staff with over 22 years combined experience including Kellish, the
Scientific Director, an immunologist with more than 7 years of experience in all aspects of flow analysis. Key
technologies available in the CYT-SR include multi-parameter flow cytometry, cell sorting, live cell imaging via
confocal microscopy, surface biomarker-based cancer and immune cell phenotyping, assessment of tissue and
cell morphology by confocal microscopy, and small particle and exosome analysis. During the recent COVID-19
pandemic, the CYT-SR was identified as an essential campus resource and remained open and accessible to
all users. Instrument reservations, equipment usage time, and technician-assisted activities are tracked by the
UFHCC and charged back via the CrossLab laboratory management system. Since 2016, the CYT-SR was
utilized by 78 unique UFHCC users (43 with peer-reviewed funding) accounting for ~24% of all users of the
facility. CYT-SR use by UFHCC members increased 5.3-fold from 900 hours in 2016 to 4,800 hours in 2021.
The CYT-SR supported more than 180 publications, with 24% having IF >10. Since the 2019 CCSG submission,
484 students, postdoctoral fellows, and technical staff from UFHCC member laboratories were trained on CYT-
SR instruments. Mechanisms of Oncogenesis and the Cancer Therapeutics and Host Response members
represent the majority of UFHCC users. The effectiveness of the CYT-SR in enhancing member research
productivity is assessed by annual surveys, feedback from the CYT-SR Scientific Advisory Group, and
recommendations from the SR Oversight Committee. Due to the rising demand for CYT-SR resources by
members, the UFHCC supported the purchase of a new Sony SP6800 Spectral Analyzer (2018), Cytoflex LX21
(2020), and a Becton Dickinson SYMPHONY A3 (2020). To support first-in-human RNA vaccine clinical trials,
UFHCC supported the recruitment of a PhD-level immunologist to the CYT-SR to perform flow-based
immunophenotyping. Future growth of the CYT-SR are in alignment with the UFHCC strategic plan, Momentum
2027, and user satisfaction surveys, and include upgrades such as an acquisition of a BD FACSYMPHONY S6
cell sorter, increasing capabilities and services in super high-resolution microscopy and single cell analysis, and
in collaboration with BQS-SR, expansion of advanced training in the application of biostatistics to cytometric
analysis.
Terms: <Abbreviations><Accounting><Adherence><Antibodies><Applications Grants><Back><Biological Markers><Biometrics><Biometry><Biostatistics><Biotech><Biotechnology><Body Tissues><Brain><Brain Nervous System><CCSG><COVID crisis><COVID epidemic><COVID pandemic><COVID-19 crisis><COVID-19 epidemic><COVID-19 global health crisis><COVID-19 global pandemic><COVID-19 health crisis><COVID-19 pandemic><COVID-19 public health crisis><COVID19 crisis><COVID19 epidemic><COVID19 global health crisis><COVID19 global pandemic><COVID19 health crisis><COVID19 pandemic><COVID19 public health crisis><Cancer Center><Cancer Center Support Grant><Cancer Control><Cancer Control Science><Cancers><Cell Body><Cell Isolation><Cell Segregation><Cell Separation><Cell Separation Technology><Cell Surface Antigens><Cells><Cellular Morphology><Center for Cancer Research><Certification><Charge><Clinical><Collaborations><Communities><Complex><Confocal Microscopy><Consultations><Data><Data Analyses><Data Analysis><Development><Doctor of Philosophy><Dorsum><Educational workshop><Effectiveness><Encephalon><Ensure><Equipment><Experimental Designs><Feedback><Florida><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Microfluorimetry><Flow Microfluorometry><Funding><Future><Generalized Growth><Genetic Research><Grant Proposals><Growth><Health><Hour><Image><Immune><Immune mediated therapy><Immune response><Immunes><Immunobiology><Immunologic Subtyping><Immunologic Surface Markers><Immunological Surface Markers><Immunological response><Immunologically Directed Therapy><Immunologist><Immunophenotyping><Immunophysiology><Immunotherapy><Infrastructure><Institution><Knowledge><Laboratories><Laser Electromagnetic><Laser Radiation><Lasers><Last Name><Letters><Malignant Neoplasms><Malignant Tumor><Methods><Microscopy><Mission><Modernization><NCI Center for Cancer Research><NIH><National Institutes of Health><Oncogenesis><Peer Review><Ph.D.><PhD><Phenotype><Policies><Population Sciences><Postdoc><Postdoctoral Fellow><Procedures><Productivity><Publications><R-Series Research Projects><R01 Mechanism><R01 Program><RNA vaccine><RNA-based vaccine><Recommendation><Reproducibility><Research><Research Activity><Research Associate><Research Grants><Research Project Grants><Research Projects><Research Resources><Research Support><Reservations><Resource Sharing><Resources><SARS-CoV-2 epidemic><SARS-CoV-2 global health crisis><SARS-CoV-2 global pandemic><SARS-CoV-2 pandemic><SARS-CoV2 epidemic><SARS-CoV2 pandemic><SARS-coronavirus-2 epidemic><SARS-coronavirus-2 pandemic><Sampling><Scanning><Science><Scientific Publication><Services><Severe Acute Respiratory Syndrome CoV 2 epidemic><Severe Acute Respiratory Syndrome CoV 2 pandemic><Severe acute respiratory syndrome coronavirus 2 epidemic><Severe acute respiratory syndrome coronavirus 2 pandemic><Solid Neoplasm><Solid Tumor><Sorting><Strategic Planning><Students><Surface><Surface Antigens><Survey Instrument><Surveys><System><Technology><Tissue Growth><Tissues><Training><UV laboratory microscope><Ultraviolet Microscopes><United States National Institutes of Health><Universities><Vaccine Clinical Trial><Vendor><Workshop><anti-cancer research><anticancer research><bio-markers><biologic marker><biomarker><cancer research><cell morphology><cell sorting><consultation><corona virus disease 2019 epidemic><corona virus disease 2019 pandemic><coronavirus disease 2019 crisis><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 pandemic><coronavirus disease 2019 public health crisis><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease pandemic><coronavirus disease-19 global pandemic><coronavirus disease-19 pandemic><cost><cost effective><data interpretation><design><designing><developmental><exosome><experience><first in man><first-in-human><flow cytophotometry><fluorescence microscope><fluorescence/UV microscope><fluorescent microscope><host response><imaging><immune system response><immune therapeutic approach><immune therapeutic interventions><immune therapeutic regimens><immune therapeutic strategy><immune therapy><immune-based therapies><immune-based treatments><immuno therapy><immunophenotype><immunoresponse><improved><instrument><instrumentation><laboratory fluorescence light microscope><live cell image><live cell imaging><live cellular image><live cellular imaging><mRNA vaccine><mRNA-based vaccine><malignancy><member><multi-photon><neoplasm/cancer><ontogeny><operation><operations><particle><post-doc><post-doctoral><post-doctoral trainee><programs><recruit><research associates><response><response to therapy><response to treatment><satisfaction><severe acute respiratory syndrome coronavirus 2 global health crisis><severe acute respiratory syndrome coronavirus 2 global pandemic><single cell analysis><student training><super high resolution><superresolution><therapeutic response><therapy response><time use><treatment response><tumorigenesis><ultra high resolution>