Calcium coding mechanisms in plant cell growth and immunity
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Principal Investigator: Sheng Luan Organization: UNIVERSITY OF CALIFORNIA BERKELEY Fiscal Year: 2022 Award: $66,680 Funding agency: National Institute of General Medical Sciences This equipment supplement application seeks funding for replacement of a broken growth chamber for culturing plant materials used in the parent project. The model plant Arabidopsis must be grown and maintained in a controlled environments. In particular, our plants are routinely transgenic containing foreign DNAs and considered “genetically modified organisms” (GMOs) and thus must be contained in a specified chamber. We are also working with pathogenic bacteria that induce immune responses in plants. Such pathogens also require containment. We have been using the Conviron plant growth chambers for these critical purposes. We had two old growth chambers (27 years) at the beginning of the parent project. An accidental floor flooding and leak above the growth chamber facility occurred and one of the growth chambers was severely damaged and we requested funding through the same supplement program in 2021 to replace it. In February 2022, the other old chamber failed due to old age. Two growth chambers are minimally required to grow plants with and without pathogen treatments. I thus request funding herein to purchase a new chamber to replace the failed chamber to ensure steady progress of the project and reduce the chance of cross-contamination of plant materials. To ensure the growth chamber operates correctly and are maintained properly, we propose to purchase the growth chamber from the same company Conviron so that our lab is familiar with the use of the chamber. Our University greenhouse and facility staff team will commit to the preventive maintenance of the growth chamber. We expect to have the new chamber in use as early as August 2022 should the funding become available in July. The two new growth chambers will provide a solid foundation for supplying plant materials in this funding period and future renewal of the project. Terms: <65+ years old><Aged 65 and Over><Animals><Arabidopsis><Calcium><Calcium Ion Signaling><Calcium Signaling><Cellular Expansion><Cellular Growth><Code><Coding System><Containment><Controlled Environment><DNA><Deoxyribonucleic Acid><Ensure><Equipment><Eukaryota><Eukaryote><Flooding><Floods><Floor><Foundations><Funding><Future><GMO Organisms><Generalized Growth><Genetically Modified Organisms><Growth><Health><Human><Immune response><Immunity><Immunological response><Intracellular Second Messenger><Maintenance><Modern Man><Organism-Level Process><Organismal Process><Physiologic Processes><Physiological Processes><Plant Model><Plants><Preventive><Second Messenger Systems><Second Messengers><Solid><Specific qualifier value><Specified><Tissue Growth><Transgenic Organisms><Universities><age 65 and greater><age 65 and older><aged 65 and greater><aged ≥65><artificial environment><bacteria pathogen><bacterial pathogen><cell growth><genetic approach><genetic strategy><host response><human old age (65+)><immune system response><immunoresponse><neuron development><neuronal development><new growth><old age><ontogeny><parent project><pathogen><pathogenic bacteria><plant development><plant growth><plant growth/development><programs><signal transduction second messengers><transgenic>