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Principal Investigator: Yi Zeng
Organization: UNIVERSITY OF ARIZONA
Fiscal Year: 2019
Award: $84,504
Funding agency: National Cancer Institute
Project Summary / Abstract: Flow Cytometry Shared Resource (FCSR)
The goal of the Flow Cytometry Shared Resource (FCSR), which has served investigators of the University of
Arizona Cancer Center (UACC) for more than 25 years, is to offer UACC Members efficient and reliable flow
cytometric services with a high standard of quality control and to do so in a cost-effective manner. The FCSR
provides the knowledge and assistance necessary to design experiments, develop new assays, perform flow
cytometric functions, and interpret data. In 2004, this service merged with Arizona Research Laboratories
(ARL) flow cytometry service, making the UACC FCSR the only University of Arizona research flow cytometry
shared resource, and the FCSR also houses the only cell sorter on the UA Tucson campus. The partnership
with the ARL has resulted in strong institutional commitment to the FCSR enabling the purchase of new
equipment and support of service contracts, as well as maximizing the utilization of resources including
staffing. The FCSR provides the following services: 1) flow cytometric analysis; 2) high-speed aseptic cell
sorting; 3) user-based (unassisted) flow cytometric analysis; 4) assisted flow cytometric analysis; 5) quality
control / quality assurance; 6) data storage, management and analysis; 7) consultation in experimental design
and data interpretation. Within these categories of service, the FCSR provides state-of-the-art analysis of cell
surface proteins on primary human and murine cells and cultured cell lines. It offers detection of cancer
chemotherapeutic agents that are fluorescent (e.g. doxorubicin), reporter dyes, intracellular pH and calcium
measurement, cell cycle analysis, apoptosis, autophagy, membrane potential, intracellular detection of
proteins, DNA ploidy analysis, cell proliferation, cytokine detection, cell sorting, and more. The FSCR also
works to facilitate projects that require integration of multiple core resources (e.g., cell sorting followed by
downstream genomic analysis). To inform investigators on new developments in flow cytometry techniques,
the FCSR regularly sponsors educational and training seminars. Hands-on-training is also provided for
students to learn antibody-based sample preparation and optimization, instrumentation operation, and data
analysis. The FCSR is Good Laboratory Practice and Biosafety Level 2 certified.
Terms: <14-Hydroxydaunomycin><Adriamycine><Antibodies><Apoptosis><Apoptosis Pathway><Arizona><Assay><Autophagocytosis><Awareness><Bioassay><Biologic Assays><Biological Assay><CCSG><Calcium><Cancer Center><Cancer Center Support Grant><Cancer Detection><Cancer Treatment><Categories><Cell Body><Cell Cycle><Cell Division Cycle><Cell Growth in Number><Cell Isolation><Cell Line><Cell Multiplication><Cell Proliferation><Cell Segregation><Cell Separation><Cell Separation Technology><Cell Surface Proteins><Cell surface><CellLine><Cells><Cellular Proliferation><Coloring Agents><Consultations><Contract Services><DNA Ploidy Analysis><Data><Data Analyses><Data Analysis><Data Storage and Retrieval><Detection><Development><Doxorubicin><Doxorubicina><Dyes><Education><Education and Training><Educational aspects><Equipment><Experimental Designs><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Cytometry Shared Resource><Flow Microfluorimetry><Flow Microfluorometry><Fluorescence Agents><Fluorescent Agents><Fluorescent Dyes><Genomics><Goals><Human><Hydroxyl Daunorubicin><Hydroxyldaunorubicin><Immunologic Subtyping><Immunophenotyping><Investigators><Knowledge><Laboratory Research><Learning><Malignant Neoplasm Therapy><Malignant Neoplasm Treatment><Measurement><Membrane><Membrane Potentials><Methodology><Methods><Mice><Mice Mammals><Modern Man><Murine><Mus><Population><Preparation><Programmed Cell Death><Proteins><Protocol><Protocols documentation><Quality Control><Reporter><Research><Research Personnel><Research Resources><Researchers><Resource Sharing><Resources><Resting Potentials><Sampling><Services><Side><Sorting - Cell Movement><Speed><Staining method><Stains><Strains Cell Lines><Students><Techniques><Technology><Training><Training and Education><Transmembrane Potentials><Universities><Work><anti-cancer research><anti-cancer therapy><anticancer research><anticancer therapy><autophagy><base><cancer prevention><cancer research><cancer therapy><cell sorting><chemotherapeutic agent><cost effective><cultured cell line><cytokine><data interpretation><data retrieval><data storage><design><designing><developmental><experiment><experimental research><experimental study><flow cytophotometry><fluorescent dye/probe><good laboratory practice><high standard><immunophenotype><innovate><innovation><innovative><instrumentation><member><membrane structure><novel><operation><quality assurance><sorting><translational study>