Investigating the role of sleep in synaptic reorganization after neural injury

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Principal Investigator: Jeffrey Michael Donlea
Organization: UNIVERSITY OF CALIFORNIA LOS ANGELES
Fiscal Year: 2024
Award: $378,300
Funding agency: National Institute of Neurological Disorders and Stroke

Project Summary
Sleep has been identified as a state of heightened neural plasticity, but the rules that govern reorganization
during sleep remain unknown. To better identify the conditions under which synapses are pruned during sleep,
we have begun to examine responses to neural injury in the fruit fly. After antennal transection, flies acutely
increase their sleep for up to one day before returning back to baseline levels. Our preliminary data also show
that pre-synaptic active zones are removed more rapidly than plasma membrane after antennal injury, and that
sleep deprivation after injury prevents the clearance of pre-synapses from injured olfactory receptor neurons.
In this project, we will examine: (1) signals that are generated after injuries to promote sleep; (2) contributions
of glial cell types to sleep regulation and synapse removal after injury; and (3) molecular mechanisms that
promote synapse removal during sleep. We anticipate that these experiments will provide insight into the role
for sleep in response to neural trauma, and begin to investigate the consequences of disrupted sleep on
recovery from axotomy.

Terms: <21+ years old><Abscission><Acute><Adult><Adult Human><Assay><Astrocytes><Astrocytus><Astroglia><Axon><Axotomy><Back><Bioassay><Biological Assay><Brain><Brain Nervous System><CNS plasticity><Cell Communication and Signaling><Cell Signaling><Cell membrane><Cytoplasmic Membrane><Data><Development><Dorsum><Drosophila><Drosophila genus><Drosophila melanogaster><Encephalon><Endocytosis><Excision><Extirpation><Flies><Gene Expression><Genes><Genetic><Genetic Alteration><Genetic Change><Genetic defect><Glia><Glial Cells><Hortega cell><Injury><Insomnia><Insomnia Disorder><Intervention><Intervention Strategies><Intracellular Communication and Signaling><Kolliker's reticulum><Libraries><Mediating><Membrane><Microglia><Modeling><Molecular><Mushroom Bodies><Mutation><Nerve><Nerve Cells><Nerve Degeneration><Nerve Transmitter Substances><Nerve Unit><Neural Cell><Neurites><Neurocyte><Neuroglia><Neuroglial Cells><Neuron Degeneration><Neuronal Injury><Neuronal Plasticity><Neurons><Neuropeptides><Neurophysiology - biologic function><Neurotransmitters><Non-neuronal cell><Nonneuronal cell><Olfactory Receptor Neurons><Peptide Receptor><Peptides><Phagocytes><Phagocytic Cell><Phagocytosis><Plasma Membrane><Process><Proteins><Public Health><Receptor Protein><Recovery><Regulator Genes><Regulatory Protein><Removal><Role><Signal Transduction><Signal Transduction Systems><Signaling><Sleep><Sleep Deprivation><Sleep disturbances><Sleeplessness><Surgical Removal><Synapses><Synaptic><Synaptic plasticity><Tachykinin><Testing><Transcriptional Regulatory Elements><Trauma><Wallerian Degeneration><aberrant sleep><adulthood><amebocyte><astrocytic glia><axon damage><axon injury><axonal damage><axonal degeneration><axonal injury><biological signal transduction><cell type><central nervous system plasticity><confocal imaging><deficient sleep><degenerative axon><developmental><disrupted sleep><disturbed sleep><experiment><experimental research><experimental study><experiments><fly><fruit fly><genetic regulatory protein><genome mutation><gitter cell><impaired sleep><inadequate sleep><injured><injuries><injury response><insight><insufficient sleep><interventional strategy><irregular sleep><membrane structure><memory encoding><mesoglia><microglial cell><microgliocyte><mutant><nerve cement><nerve injury><nerve transection><neural><neural degeneration><neural function><neural injury><neural mechanism><neural plasticity><neurochemical><neurochemistry><neurodegeneration><neurodegenerative><neurological degeneration><neuromechanism><neuron injury><neuronal><neuronal degeneration><neuroplastic><neuroplasticity><neuroprotection><neuroprotective><perivascular glial cell><plasmalemma><presynaptic><prevent><preventing><programs><receptor><regulatory gene><regulatory gene product><resection><response><response to injury><secondary degeneration><sleep control><sleep debt><sleep deficiency><sleep deficit><sleep disruption><sleep dysregulation><sleep insufficiency><sleep loss><sleep regulation><social role><synapse><synaptic pruning><synthetic enzyme><trans acting element>