Molecular imaging of viral infections

NIH Pandemic-Era Grants

Pandemic Era Grants

2021

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Principal Investigator: Dima A Hammoud
Organization: CLINICAL CENTER
Fiscal Year: 2021
Funding agency: NIH Clinical Center

Sars-Cov-2 project:
We have started our experiments by developing the LPS lung injury model by administering LPS intratracheally. We are using male and female mice, including WT and ACE2 Transgenic mice. At this stage we are imaging the lung infiltrates and assessing ACE2 levels in the various groups by western and real-time qPCR. We are hoping to perform a detailed analysis of all the experiments in a few months. 

HIV project: The work has not been started yet. A protocol was submitted to ACUC and approved. We are planning on starting the animal models development soon. We are also awaiting the synthesis and affinity evaluation of multiple nanobodies against GP120.

Ebola and other high consequence viral infections: We are collaborating with the Integrated research facility on multiple studies evaluating Marburg and Ebola infections.

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2><SARSCoV2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 S protein><Severe acute respiratory syndrome coronavirus 2 infection><Severe acute respiratory syndrome coronavirus 2 spike glycoprotein><Severe acute respiratory syndrome coronavirus 2 spike protein><Severe acute respiratory syndrome related corona virus 2><Severities><Site><Therapeutic Intervention><Time><Tissues><Tomodensitometry><Transgenic Mice><Viral><Viral Diseases><Virus><Virus Diseases><Virus Replication><Virus-HIV><Wild Type 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modality><improved><in vivo><in vivo imaging><infected with COVID-19><infected with COVID19><infected with SARS-CoV-2><infected with SARS-CoV2><infected with coronavirus disease 2019><infected with severe acute respiratory syndrome coronavirus 2><injured CNS><intervention therapy><lung imaging><lung injury><lung pathology><lung scanning><male><microPET/CT><model of animal><model organism><molecular imaging><molecule imaging><mouse model><murine model><nCoV2><nanobodies><nanobody><non-invasive imaging><noninvasive imaging><pathway><positron emission tomographic (PET) imaging><positron emission tomographic imaging><positron emitting tomography><post treatment><prevent><preventing><pulmonary><receptor><research facility><response><sdAb><single domain antibodies><social role><viral infection><viral multiplication><viral replication><virus infection><virus multiplication><virus-induced disease><wildtype mouse>