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Principal Investigator: Gregory Brion Timmel
Organization: UNIVERSITY OF CALIFORNIA AT DAVIS
Fiscal Year: 2024
Award: $888,638
Funding agency: NIH Office of the Director
Husbandry & Management Abstract
The CNPRC has maintained a pedigreed colony of Indian-origin rhesus macaques for over 22 years. The SPF
Pedigreed Indian origin rhesus colony includes both an SPF level 1 colony negative for Simian Retrovirus
(SRV), Simian Immmunodeficiency Virus (SIV), Simian T Cell Lymphotrophic Virus (STLV) , and Herpes B virus
(B virus) and an SPF Level 2 colony free of three additional viruses including rhesus cytomegalovirus
(RhCMV), simian foamy virus (SFV), and rhesus rhadinovirus (RRV). This application will expand the
percentage of SPF Level 2 rhesus while maintaining the current overall colony size. We will continue pedigree
determination for all colony animals and characterize the Major Histocompatibility Complex (MHC) Class I
(Mamu-A and -B) and Class II (Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci in our breeding SPF rhesus
macaques. This application will continue to collect fecal microbiome and peripheral blood lymphoid cells as a
biorepository for use by HIV/AIDS investigators. The four principal goals of this application are:
Continue to expand the SPF Level 2 population relative to Level 1 while maintaining overall colony size.
Develop improved and expanded screening and confirmatory tests for the detection of SPF agents to facilitate
the maintenance of the SPF colony. We will initiate annual surveillance for Trypanosoma cruzi in recognition of
the expansion of the geographic range of this parasitic infection. This work includes new assays for agents
such as tuberculosis, which represents the first SPF agent.
Maintain genetic analysis to determine the pedigree of Indian rhesus born into the colony and test all adult
breeders to characterize their Major Histocompatibility Complex (MHC) Class I (Mamu-A and -B) and Class II
(Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci.
Continue the collection and storage of intestinal microbiome and peripheral blood lymphocyte samples in a
unique SPF Level 2 biorepository to facilitate future research projects investigating the interaction of HIV with
the host microbiome and immune system.
Terms: <21+ years old><AIDS><AIDS Virus><AIDS/HIV><Acquired Immune Deficiency><Acquired Immune Deficiency Syndrome><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome><Acquired Immunodeficiency Syndrome Virus><Adult><Adult Human><American trypanosome><Animals><Assay><Bioassay><Biological Assay><Breeding><CMV><COVID surveillance><COVID-19 surveillance><COVID19 surveillance><California><Censuses><Collection><Cytomegalovirus><GI microbiome><Genetic analyses><Geography><Goals><HCMV><HIV><HIV Infections><HIV/AIDS><HTLV-III Infections><HTLV-III-LAV Infections><Histocompatibility Complex><Histocompatibility Complices><Human Immunodeficiency Viruses><Human T-Lymphotropic Virus Type III Infections><Immune response><Immune system><Immunological response><Inflammation><Investigators><LAV-HTLV-III><Lymphadenopathy-Associated Virus><Lymphoid Cell><M mulatta><M tuberculosis infection><M. mulatta><M. tb infection><M. tuberculosis infection><M.tb infection><M.tuberculosis infection><MTB infection><Macaca mulatta><Maintenance><Major Histocompatibility Complex><Major Histocompatibility Complices><Modeling><Mycobacterium tuberculosis (MTB) infection><Mycobacterium tuberculosis infection><Parasitic infection><Pedigree><Peripheral Blood Lymphocyte><Population><Primates><Primates Mammals><Production><R-Series Research Projects><R01 Mechanism><R01 Program><Research><Research Grants><Research Personnel><Research Project Grants><Research Projects><Research Resources><Researchers><Resources><Rhadinovirus><Rhesus><Rhesus Macaque><Rhesus Monkey><SARS-CoV-2 surveillance><SIV><Salivary Gland Viruses><Sampling><Simian Foamy Virus><Simian Immunodeficiency Viruses><Simian Retroviruses><T cruzi><T. cruzi><TB infection><Testing><Trypanosoma cruzi><Tuberculosis><Virus><Virus-HIV><Work><adulthood><biobank><biorepository><coronavirus disease 2019 surveillance><coronavirus disease surveillance><cytomegalovirus group><detection test><detection tests><digestive tract microbiome><disseminated TB><disseminated tuberculosis><enteric microbiome><fecal microbiome><flexibility><flexible><gastrointestinal microbiome><genetic analysis><genetic pedigree><gut microbiome><gut-associated microbiome><host microbiome><host response><immune system response><immunoresponse><improved><infection due to Mycobacterium tuberculosis><intestinal biome><intestinal microbiome><parasite infection><pathogen><pedigree structure><peripheral blood><screening><screenings><severe acute respiratory syndrome coronavirus 2 surveillance><simian T cell lymphotrophic virus><stool microbiome><stool-associated microbiome><tuberculosis infection><tuberculous spondyloarthropathy>