Using single-cell RNA-seq to interrogate host immunity to pathogens

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Principal Investigator: John T Chang
Organization: UNIVERSITY OF CALIFORNIA, SAN DIEGO
Fiscal Year: 2020
Award: $557,572
Funding agency: National Institute of Allergy and Infectious Diseases

Project Summary
 Mortality from infectious diseases remains a leading cause of death worldwide,
making the development of new vaccines an important priority of biomedical research.
Immunologic memory is a cardinal feature of adaptive immunity and an important goal of
vaccination strategies. Traditional vaccination strategies are very effective at generating
neutralizing antibodies against bacteria and viruses. However, a vaccine capable of
generating robust T lymphocyte memory is still beyond our research, due, in part, to an
incomplete understanding of the molecular basis of lymphocyte fate specification. In this
proposal, we will develop single-cell approaches to study specification of lymphocyte
fates in response to microbial infection.

Terms: <Acute><Bacteria><Bacterial Infections><Biomedical Research><CD8 Cell><CD8 T cells><CD8 lymphocyte><CD8+ T cell><CD8+ T-Lymphocyte><CD8-Positive Lymphocytes><CD8-Positive T-Lymphocytes><Cause of Death><Cell Body><Cells><Communicable Diseases><Development><Exhibits><Gene Expression Monitoring><Gene Expression Pattern Analysis><Gene Expression Profiling><Genetic><Goals><Heterogeneity><Immune memory><Immune system><Immunity><Immunologic Memory><Immunological Memory><Individual><Infection><Infectious Disease Pathway><Infectious Diseases><Infectious Disorder><Knock-out><Knockout><L monocytogenes><L. monocytogenes><Listeria monocytogenes><Lymphocyte><Lymphocytic><Memory><Molecular><Population><RNA Computations><Regulator Genes><Research><SCmRNAseq><Systemic infection><T memory cell><T-Cell Subsets><T-Cells><T-Lymphocyte><T-Lymphocyte Subsets><T8 Cells><T8 Lymphocytes><TEM cells><Transcript Expression Analyses><Transcript Expression Analysis><Transcription Regulation><Transcriptional Control><Transcriptional Regulation><Transcriptional Regulatory Elements><Vaccine Design><Vaccines><Virus><adaptive immune response><adaptive immunity><allergic/immunologic body system><allergic/immunologic organ system><anamnestic reaction><bacteria infection><bacterial disease><base><design><designing><developmental><gene expression analysis><gene expression assay><global gene expression><global transcription profile><improved><in vivo><infection mouth><insight><knock-down><knockdown><lymph cell><memory T lymphocyte><microbial><mortality><neutralizing antibody><new vaccines><next generation vaccines><novel><novel vaccines><oral infection><oral infectious><pathogen><regulatory gene><response><scRNA-seq><secondary immune response><shRNA><short hairpin RNA><single cell RNA-seq><single cell RNAseq><single cell mRNA sequencing><single-cell RNA sequencing><small hairpin RNA><terminally differentiated effector memory (TEM) T cells><terminally differentiated effector memory T cells><thymus derived lymphocyte><trans acting element><transcriptional profiling><transcriptome><transcriptomics><vaccination strategy>