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Principal Investigator: MARY ANN Y JABRA-RIZK
Organization: UNIVERSITY OF MARYLAND BALTIMORE
Fiscal Year: 2022
Award: $231,750
Funding agency: National Institute of Dental and Craniofacial Research
PROJECT SUMMARY
Recently, individuals with COVID-19 were found to harbor replicating SARS-CoV-2 inside
salivary epithelial cells resulting in inflammation, architectural distortion, and atrophy of minor
and major salivary glands. We have previously demonstrated the importance of histatin-5, a
secreted salivary antimicrobial peptide exclusively produced in the salivary glands, in protecting
the oral cavity against the opportunistic pathogen Candida albicans. In light of the findings
indicating that COVID-19 patients likely suffer from salivary gland dysfunction, we hypothesize
that histatin-5 salivary levels are compromised in COVID-19 patients potentially predisposing
these individuals to long term opportunistic infections and other oral mucosal conditions. In this
proposed clinical study, we will utilize our developed immunoassay to demonstrate the impact of
COVID-19 on histatin-5 production, Candida colonization, and induction of local immune
activation. We expect the findings generated from these studies to provide lacking mechanistic
insights into the pathophysiology of salivary gland dysfunction in COVID-19 patients, and its
implications on the health of the oral cavity.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><ACE2><AIDS Virus><Acinar Cell><Aciner Cells><Acinus organ component><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome Virus><Acute><Architecture><Asialia><Atrophic><Atrophy><Body Tissues><Buccal Cavity><Buccal Cavity Head and Neck><Buccal Mucosa><C albicans><C. albicans><C.albicans><COVID infected patient><COVID patient><COVID positive patient><COVID-19><COVID-19 affected><COVID-19 consequence><COVID-19 effect><COVID-19 impact><COVID-19 impacted><COVID-19 infected patient><COVID-19 infection><COVID-19 patient><COVID-19 positive patient><COVID-19 virus><COVID19><COVID19 infection><COVID19 patient><COVID19 positive patient><COVID19 virus><CV-19><CV19><Candida><Candida albicans><Candidiasis><Candidosis><Causality><Cavitas Oris><Cell Body><Cells><Chronic><Clinical Research><Clinical Study><CoV-2><CoV2><Data Set><Dataset><Disease><Disorder><Duct><Duct (organ) structure><Ductal Cell><Ductal Epithelial Cell><Dysfunction><Engineering / Architecture><Epithelial Cells><Etiology><Exocytosis><Functional disorder><Goals><HIV><HIV Infections><HTLV-III Infections><HTLV-III-LAV Infections><Human><Human Immunodeficiency Viruses><Human T-Lymphotropic Virus Type III Infections><Hyposalivation><Immune Cell Activation><Immunoassay><Immunologic Factors><Immunological Factors><In Vitro><Individual><Infection><Inflammation><Inflammatory><Innate Immune System><Intercalated Duct><LAV-HTLV-III><Lead><Light><Lymphadenopathy-Associated Virus><Lymphatic cell><Lymphocyte><Lymphocytic><Major Salivary Gland><Major salivary gland structure><Mediating><Microbiology><Minor><Modern Man><Monilia><Moniliasis><Mouth><Mouth Dryness><Mouth Mucosa><Mouth Ulcer><Mucosa><Mucosal Tissue><Mucous Membrane><Mucous body substance><Mucus><Opportunistic Infections><Oral><Oral Manifestations><Oral Moniliasis><Oral Mucosa><Oral Ulcer><Oral candidiasis><Oral cavity><Oral health><Oral mucous membrane structure><Patients><Pb element><Photoradiation><Physiologic><Physiological><Physiopathology><Process><Production><Receptor Protein><Reporting><Risk><Role><Rupture><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV-2 infected patient><SARS-CoV-2 infection><SARS-CoV-2 patient><SARS-CoV-2 positive patient><SARS-CoV2><SARS-CoV2 infection><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Saliva><Salivary><Salivary Glands><Salivary Glands Head and Neck><Secretory Granules><Secretory Vesicles><Serous><Serous Salivary Gland><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 infection><Severe acute respiratory syndrome related corona virus 2><Sialadenitis><Sialoadenitis><Site><Symptoms><Thrush><Tissues><Viral><Viral Burden><Viral Load><Viral Load result><Virus-HIV><Wound Repair><Wuhan coronavirus><Xerostomia><acinus><angiotensin converting enzyme 2><angiotensin converting enzyme II><anti-microbial peptide><antimicrobial peptide><aptyalism><causation><cohort><corona virus disease 2019><coronavirus disease 2019><coronavirus disease 2019 consequence><coronavirus disease 2019 effect><coronavirus disease 2019 impact><coronavirus disease 2019 infected patient><coronavirus disease 2019 infection><coronavirus disease 2019 patient><coronavirus disease 2019 positive patient><coronavirus disease 2019 virus><coronavirus disease infected patient><coronavirus disease patient><coronavirus disease positive patient><coronavirus disease-19><coronavirus disease-19 impact><coronavirus disease-19 patient><coronavirus disease-19 virus><coronavirus infectious disease-19><coronavirus patient><dental health><disease causation><dry mouth><fungal pathogen><fungi pathogen><hCoV19><heavy metal Pb><heavy metal lead><histatin 5><histatins><histidine-rich proteins><immune activation><immunologic substance><immunological substance><in vivo><infected with COVID-19><infected with COVID19><infected with SARS-CoV-2><infected with SARS-CoV2><infected with coronavirus disease 2019><infected with severe acute respiratory syndrome coronavirus 2><inflammation marker><inflammatory marker><insight><lymph cell><microbial colonization><mouth candida><mucous><mycotic stomatitis><nCoV2><novel><opportunistic pathogen><oral candida><oral mucosae><oral mucosal><pathogenic fungus><pathophysiology><patient infected with COVID><patient infected with COVID-19><patient infected with SARS-CoV-2><patient infected with coronavirus disease><patient infected with coronavirus disease 2019><patient infected with severe acute respiratory syndrome coronavirus 2><patient population><patient with COVID><patient with COVID-19><patient with COVID19><patient with SARS-CoV-2><patient with coronavirus disease><patient with coronavirus disease 2019><patient with severe acute respiratory distress syndrome coronavirus 2><post-COVID><post-COVID-19><post-coronavirus disease 2019><receptor><salivary cell><salivary gland inflammation><salivary histatin 5><scRNA-seq><severe acute respiratory syndrome coronavirus 2 infected patient><severe acute respiratory syndrome coronavirus 2 patient><severe acute respiratory syndrome coronavirus 2 positive patient><single cell RNA-seq><single cell RNAseq><single cell analysis><single cell expression profiling><single cell transcriptomic profiling><single-cell RNA sequencing><social role><wound healing><wound resolution>