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Principal Investigator: JERREL L YAKEL
Organization: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
Fiscal Year: 2023
Award: $1,741,729
Funding agency: National Institute of Environmental Health Sciences
The Viral Vector Core facility currently offers support for production, purification, titering, and validation of twelve types of viruses: Adenovirus, Adenoassociated virus (AAV), coronaviruses (229E, OC43, NL63, and MHV), Herpes Simplex Virus (HSV), Influenza A, lentiviruses including human immunodeficiency virus (HIV), Lymphocytic choriomeningitis virus (LCMV), Rabies-delta-G (SAD-B19-dG, dGL, SiR, and CVS-N2c), Retrovirus, Respiratory syncytial virus (RSV), Sindbis Virus, vesicular stomatitis virus (VSV) and Zika virus. The core utilizes multiple vector systems to safely produce recombinant, pseudotyped and wild type viruses.
This year, the Viral Vector Core provided services for propagation and transduction assays using SARS coronavirus and SARS coronavirus 2 spike pseudotyped virions and coronavirus strains 229E, OC43, NL63, and MHV. The Viral Vector Core offers SARS coronavirus and SARS coronavirus 2 Wuhan, UK (alpha), South Africa (beta), Brazil, India (delta), and Omicron spike variant pseudotyped lentiviruses and VSVs. These virions were used in transduction assays to screen a multitude of anti-viral reagents. SARS-CoV-2 pseudotyped virions were also utilized for structural studies of the virus spike.
In addition, the core facility developed transduction protocols for gene delivery to primary or immortalized cell lines, mouse fertilized eggs, and animal tissue. In the past year, the Viral Vector Core has packaged and validated over 330 viral samples for gene delivery. These vectors have been utilized in research to express ion channels, transcription factors, biological indicators, hormone receptors, secreted protein expression and purification, gene knockdown by shRNA or genetic modification using CRISPR components. Viral vectors were also used to introduce genes into primary and immortalized cell lines, mouse and rat tissue, or cultured tissue. Other difficult to transfect cell lines, such as hematopoietic and mouse embryonic cells, were also successfully transduced using viral vectors. Lentiviral vectors facilitated immortalizing primary cell lines for toxicological studies and several viral strains were imported for use in immunological studies.
The Viral Vector Core supports research in the Division of National Toxicology Program (DNTP), Clinical Research Branch (CRB), Epigenetic and Stem Cell Biology Laboratory (ESCBL), Genome Integrity and Structural Biology Laboratory (GISBL), Immunity, Inflammation and Disease Laboratory (IIDL), Neurobiology Laboratory (NL), Signal Transduction Laboratory (STL), and Reproductive and Developmental Biology Laboratory (RDBL) at the NIEHS by providing highly effective means of gene delivery.
The core also provided viral products for laboratories in the NIA, NIAAA, NIDDK, NIAID and NIMH.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><AIDS Virus><Accountability><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome Virus><Adeno-Associated Viruses><Adenoviridae><Adenoviruses><Assay><Basal Transcription Factor><Basal transcription factor genes><Bioassay><Biochemical><Biologic Assays><Biological><Biological Assay><Blastocytes><Body Tissues><Brazil><COVID-19 virus><COVID19 virus><CRISPR><CRISPR/Cas system><Cell Body><Cell Communication and Signaling><Cell Line><Cell Signaling><CellLine><Cells><Clinical Research><Clinical Study><Clustered Regularly Interspaced Short Palindromic Repeats><CoV-2><CoV2><Common Rat Strains><Consultations><Core Facility><Coronaviridae><Coronaviridae Infections><Coronavirus><Coronavirus Infections><Culturing, in vitro Vertebrate, Primary><Dependoparvovirus><Dependovirus><Development><Developmental Biology><Disease><Disorder><Embryonic Cell><Ensure><Epigenetic><Epigenetic Change><Epigenetic Mechanism><Epigenetic Process><Fertilized Egg><Fertilized Ovum><Gene Delivery><General Transcription Factor Gene><General Transcription Factors><Genes><Genetic><Guidelines><HCoV><HIV><HSV><Hematopoietic><Herpes Simplex Virus><Herpes labialis Virus><Hormone Receptor><Human Immunodeficiency Viruses><Immunity><Immunochemical Immunologic><Immunologic><Immunological><Immunologically><Immunologics><In Vitro><India><Inflammation><Influenza A><Influenza A virus><Influenza Virus><Influenza Viruses Type A><Influenzavirus A><Integrase><Intracellular Communication and Signaling><Investigators><Ion Channel><Ionic Channels><LAV-HTLV-III><LCM Viruses><LCMV><Laboratories><Lentiviral Vector><Lentivirinae><Lentivirus><Lentivirus Vector><Lymphadenopathy-Associated Virus><Lymphocytic choriomeningitis virus><Membrane Channels><Mice><Mice Mammals><Mission><Modification><Murine><Mus><NIAAA><NIAID><NIDDK><NIEHS><NIH><NIMH><National Institute of Allergy and Infectious Disease><National Institute of Diabetes and Digestive and Kidney Diseases><National Institute of Environmental Health Sciences><National Institute of Mental Health><National Institute on Alcohol Abuse and Alcoholism><National Institutes of Health><National Toxicology Program><Neurobiology><Orthomyxovirus Type A><Primary Cell Cultures><Principal Investigator><Production><Protein Secretion><Protocol><Protocols documentation><Rabies><Rat><Rats Mammals><Rattus><Reagent><Recombinants><Reproductive Biology><Research><Research Personnel><Research Support><Researchers><Respiratory syncytial virus><Retroviridae><Retroviruses><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CO-V2><SARS-COVID-2><SARS-CoV><SARS-CoV-1><SARS-CoV-2><SARS-CoV2><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Safety><Sampling><Services><Severe Acute Respiratory Coronavirus><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Signal Transduction><Signal Transduction Systems><Signaling><Simplexvirus><Sindbis Virus><South Africa><Strains Cell Lines><System><Technology><Time><Tissues><Toxicology><Training><Transcription Factor Proto-Oncogene><Transcription factor genes><Transfection><Transgenic Animals><Type A Influenza><United States National Institutes of Health><VSV><Validation><Variant><Variation><Vesicular Stomatitis Virus><Vesicular stomatitis Indiana virus><Viral><Viral Vector><Virion><Virus><Virus Particle><Virus-HIV><Virus-Retrovirus><Wuhan coronavirus><ZIKV><Zika Virus><adeno associated virus group><animal tissue><biologic><biological signal transduction><blastomere structure><cell immortalization><consultation><corona virus><coronavirus disease 2019 virus><coronavirus disease-19 virus><cost><cost effective><cultured cell line><developmental><embryo cell><epigenetically><genome integrity><genomic integrity><hCoV19><hemopoietic><human CoV><human corona virus><human coronavirus><in vivo><influenzavirus><knock-down><knockdown><lyssa><nCoV2><neurobiological><programs><protein expression><protein purification><severe acute respiratory syndrome-CoV><shRNA><short hairpin RNA><small hairpin RNA><stable cell line><stem cell biology><structural biology><tissue culture><transcription factor><validations><vector><viral gene delivery><zikav><zygote>