Combined archive search across FBI Vault, SPLC, PHMPT, ACIP, NIH grants, the Federal Writers' Project, and other collections we've ingested.
Peter D Kwong · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $157,844 — An understanding of the serum responses of virus-infected individuals and vaccinees is critical for the development of effective vaccines against HIV-1, SARS-CoV-2 and other viral pathogens. Perhaps the most important biological aspect of a serum response is its neutralizing activity, with a close s…
Khalid S. Salaita · EMORY UNIVERSITY · 2022 · $433,540 — Project Abstract The ultimate goal of this proposal is to develop a novel platform technology for automatic surveillance and tracing of airborne SARS-CoV-2 virus particles in real time. The centerpiece of this proposal is the “Rolosense” technology which leverages a DNA micromotor as the virus sensi…
Patrick Duffy · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $390,420 — In July 2020, we commenced a community COVID-19 seroprevalence study at existing clinical trials sites in Mali. This study is a Public Health Surveillance Activity in collaboration with the Ministry of Health in Mali to describe the sero-epidemiology of COVID-19 in urban and rural populations. Using…
Thomas Quinn · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $37,442 — Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), emerged as a global pandemic in early 2020. By the end July of 2022, over 560 million cases of SARS-CoV-2 have been confirmed, spanning almost all countries and accounting for > …
David Sacks · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $57,995 — In order to overexpress SARS CoV-2 spike protein (S) in Leishmania, the full length S gene sequence was amplified by PCR from plasmid pCAGGS containing the Wuhan-Hu-1 spike and inserted into plasmid pSSU-NEO using a Gibson Assembly reaction, with the addition of an N-terminal myc tag and a C-termi…
JERREL L YAKEL · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2022 · $1,684,646 — The Viral Vector Core facility currently offers support for production, purification, titering, and validation of twelve types of viruses: Adenovirus, Adenoassociated virus (AAV), coronaviruses (229E, OC43, NL63, and MHV), Herpes Simplex Virus (HSV), Influenza A, lentiviruses including human immunod…
Adriaan Bax · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · 2022 · $658,712 — The envelope glycoprotein gp41 of the HIV-1 virus mediates its entry into the host cell. During this process, gp41 undergoes large conformational changes and the energy released in the remodeling events is utilized to overcome the barrier associated with fusing the viral and host membranes. Although…
Joseph Shiloach · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · 2022 · $175,065 — Summary: An essential part of the Lab activities is production of unique biologicals that are not available from commercial sources at a suitable quality, for basic and clinical research. During the last year the Lab performed several processes, which include propagation of bacteria, and mammalian c…
Michael Michaelides · NATIONAL INSTITUTE ON DRUG ABUSE · 2022 · $1,981,761 — The societal and economic burden of substance use disorders is vast and ever-increasing. To address this requires developing innovative therapeutics, an approach necessitating a deep understanding of behavior and its neurobiological basis. Fortunately, the last decade has witnessed a surge of breakt…
Heather Kalish · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · 2022 · $655,495 — Sub-project #1 MALDI imaging of tissue samples to identify novel proteins MALDI-TOF Imaging is an emerging tool for the label free measurement of peptides, proteins, lipids, drugs and metabolites. The tissue can be imaged directly be the MALDI-TOF and combined with other modalities to study the mo…
Richard A Koup · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $2,480,852 — The Virology Core has collaborated with other intramural NIAID labs help to develop a pan-coronaviruses spike protein subunit vaccine. During the past year, we focused on developing the vaccine vaccines against most subecoviruses or beta-coronaviruses. We successfully constructed hybrid immunogen…
Richard A Koup · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $285,362 — Since there is no effective cure for COVID-19. Though the mRNA vaccines were authorized for emergency use, the durability of protection provided by these two mRNA vaccines is still a concern, given the fact that the spike-specific antibodies decline rapidly in convalescent sera of COVID patients. Du…
MIGUEL HOLMGREN · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · 2022 · $1,677,840 — Calcium ions increase the open probability of the large conductance potassium (BK) channels by binding to high affinity sites within the large cytosolic Ca2+ sensing structure called the gating ring. This Ca2+ sensing structure is formed by eight Regulator of K+ Conductance (RCK) domains, two from e…
Alex Compton · DIVISION OF BASIC SCIENCES - NCI · 2022 · $69,947 — We have successfully produced HIV-based and VSV-based pseudovirus bearing the spike protein of SARS-CoV-1 and SARS-CoV-2 variants and produced cell lines that are permissive to these pseudoviruses. We have developed a protocol for transiently transfecting human ACE2 (the receptor for …
PETER SCHUCK · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · 2022 · $422,189 — The COVID19 pandemic has spawned an unprecedented worldwide collaboration to collect SARS-CoV-2 genome sequences in the GISAID database. It is currently approaching a size of 10 million genomes and is rapidly growing. This genomic database has been instrumental for enhancing our understanding of vir…
Thomas Nutman · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $137,557 — In response to the SARS-CoV-2 pandemic, we leveraged our expertise in human T cell cloning to generate SARS-CoV2-specificT cell lines from naive CD4+ and CD8+ T cells of uninfected (SARS Cov2-naive) individuals. To this end, we generated S-specific, M-specific, CMV-specific (controls), CEF-specific …
Iain Fraser · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2022 · $72,177 — Cells of the innate immune system, such as dendritic cells and macrophages, constantly patrol host mucosal surfaces and peripheral tissues for signs of infection or injury. Since many people infected with SARS-CoV-2 clear the virus without developing symptoms, aspects of the innate immune response m…
Joel Moss · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · 2022 · $2,503,531 — A. A mixed blood-lymphatic endothelial cell phenotype is Lymphangioleiomyomatosis and Idiopathic Pulmonary Fibrosis but not in Kaposi's Sarcoma and Tuberous Sclerosis Complex:In LAM lungs, the walls of the cystic lesions contain LAM lung nodules, which are composed of neoplastic smooth muscle-like L…
PAUL T WINGFIELD · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · 2022 · $1,300,893 — In uninfected cells, RNA is transcribed from DNA, processed, and then transported out of the nucleus and translated into protein. In cells infected with HIV-1, the viral RNA genomes must be exported out of the nucleus without being processed so they can be packaged into new viral particles. To do th…
Naoko Mizuno · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · 2022 · $498,022 — We hypothesize that SARS-Cov2 directly interacts with neutrophils as well as platelets through their receptors, and induces a change in platelet morphology, leading to their activation and coagulation. We performed live imaging showing that platelets are directly activated in the presence of purifie…