Document text
Principal Investigator: Karen Scanlon
Organization: UNIVERSITY OF MARYLAND BALTIMORE
Fiscal Year: 2021
Award: $193,125
Funding agency: National Institute of Allergy and Infectious Diseases
PROJECT SUMMARY
Pertussis, caused by Bordetella pertussis, is a reemerging disease of major public health concern. Pertussis
severity is age-related, with B. pertussis causing severe manifestations that are unique to young infants and
not observed at an older age. These infants are at increased risk of cardiopulmonary failure and death from B.
pertussis-induced pulmonary hypertension (PH). Age-related differences in disease severity indicate that host
responses to B. pertussis differ with age. The causal mechanism for PH in severe pertussis is unknown and
there are currently no effective therapeutic interventions for the treatment of pertussis-associated PH or death.
The study proposed here aims to address this critical gap in knowledge, providing mechanistic information and
validating the use of potentially novel therapeutics for pertussis.
PH-associated cardiac dysfunction is initiated by remodeling of pulmonary arteries, but how does B.
pertussis cause this remodeling? And, why is this function age-related? In unpublished and preliminary studies,
B. pertussis was found to induce activation of the renin-angiotensin system (RAS) in the lungs of infant mice
but not adult mice and pertussis toxin (PT) expression was required for B. pertussis-induced PH in infant mice.
The RAS is one of the most important and best-studies systems in the pathogenesis of hypertension but has
not been linked to infection-induced PH. The proposed experiments will test the hypothesis that high RAS
activity in the infant lung and PT-mediated inhibition of a protective component of this system cause
pathogenic RAS signaling and initiates pathologies that drive the onset of PH. Critical points of regulation in the
RAS include the formation and degradation of angiotensin II (ANGII) by angiotensin-converting enzyme (ACE)
and ACE2 respectively and ANGII signaling via AT1 and AT2 receptors to mediate opposing actions that may
be pathogenic (AT1, vasoconstriction and proliferation) or protective (AT2, vasodilation and anti-proliferative) in
our system. Preliminary data also shows that 1) basal pulmonary ANGII levels are higher in infants than adults,
2) B. pertussis downregulates expression of the angiotensin precursor gene Agt in adult lungs but increases
expression in infant lungs, 3) B. pertussis downregulates ACE in adult lungs but not infant lungs and 4) basal
expression of AT2 is significantly greater in infant lungs than adult lungs. These data indicate that, in infants,
high AT2 levels are required to limit the effect of excessive ANGII signaling through AT1. However, AT2, but not
AT1, is susceptible to PT-mediated inhibition, hence PT produced during infection would have a greater impact
on ANGII signaling in infants than it would in adults. In aim 1, the age-related effect of B. pertussis on ANGII
peptide accumulation and ACE and ACE2 activity will be examined. Age-related expression of AT2 will also be
determined and the effect of infection of AT2 activity will be tested. In aim 2, the contribution of the RAS and
PT-mediated inhibition of AT2 on B. pertussis-induced PH and death will be resolved. If our hypothesis is
correct, RAS-targeting therapeutics may save the lives of young infants with severe pertussis.
Terms: <0-11 years old><21+ years old><ACE2><AGTR2><AGTR2 gene><AT2><Address><Adult><Adult Human><Age><AngII><Angiotensin Converting Enzyme><Angiotensin I-Converting Enzyme><Angiotensin II><Angiotensin II Receptor><Angiotensinogen><Angiotensins><Asystole><B pertussis><B pertussis infection><B. pertussis><B. pertussis infection><Bordetella pertussis><Bordetella pertussis infection><CD143 Antigens><Carboxycathepsin><Cardiac Arrest><Cardiopulmonary><Cathetergram><Catheterization><Causality><Cause of Death><Cell Communication and Signaling><Cell Signaling><Cessation of life><Child><Child Youth><Children (0-21)><Coupled><Data><Death><Development><Dipeptidyl Peptidase A><Disease><Disorder><Echocardiogram><Echocardiography><Etiology><Exposure to><Failure><Fatal Outcome><G Protein-Complex Receptor><G Protein-Coupled Receptor Genes><G Protein-Coupled Receptor Signaling><G-Protein-Coupled Receptors><GPCR><GPCR Signaling><Genes><H Pertussis><H. Pertussis><Haemophilus pertussis><Health><Heart><Heart Arrest><Heart failure><Histamine-Sensitizing Factor><Hypertensinogen><Hypertension><IAP Pertussis Toxin><Immune response><Immunological response><In Vitro><Infant><Infection><Infectious Agent><Intracellular Communication and Signaling><Islet-Activating Protein><Kininase A><Kininase II><Knowledge><Link><Lung><Lung Respiratory System><Lymphocytosis-Promoting Factor><Mediating><Mice><Mice Mammals><Modeling><Murine><Mus><Myocardial depression><Myocardial dysfunction><Pathogenesis><Pathogenicity><Pathology><Pathway interactions><Peptides><Peptidyl-Dipeptidase A><Pertussigen><Pertussis><Pertussis Toxin><Proangiotensin><Production><Public Health><Pulmonary Artery><Pulmonary Hypertension><Pulmonary artery structure><Receptor Protein><Refractory><Regulation><Renin-Angiotensin System><Renin-Substrate><Research><Right Ventricles><Right ventricular structure><Risk><Severities><Severity of illness><Signal Transduction><Signal Transduction Systems><Signaling><System><Testing><Therapeutic Agents><Therapeutic Intervention><Transcript><Transthoracic Echocardiography><Vascular Hypertensive Disease><Vascular Hypertensive Disorder><Vasodilatation><Vasodilation><Vasorelaxation><Whooping Cough><adulthood><age dependent><age related><ages><angiotensin converting enzyme 2><angiotensin converting enzyme II><biological signal transduction><cardiac dysfunction><cardiac failure><causation><death risk><developmental><disease causation><disease severity><effective therapy><effective treatment><experiment><experimental research><experimental study><global gene expression><global transcription profile><heart dysfunction><heart sonography><high blood pressure><host response><hyperpiesia><hyperpiesis><hypertensive disease><immune system response><immunoresponse><improved><in vivo><infant infection><infected infant><infected with B pertussis><infected with B. pertussis><infected with Burkholderia pertussis><infectious organism><intervention therapy><mortality risk><mouse model><murine model><new approaches><new drug treatments><new drugs><new therapeutics><new therapy><next generation therapeutics><novel approaches><novel drug treatments><novel drugs><novel strategies><novel strategy><novel therapeutics><novel therapy><pathway><postnatal><pulmonary><receptor><targeted drug therapy><targeted drug treatments><targeted therapeutic><targeted therapeutic agents><targeted therapy><targeted treatment><therapeutically effective><transcriptome><treatment strategy><vascular constriction><vasoconstriction><youngster>