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Principal Investigator: Rachel M Olson
Organization: UNIVERSITY OF MISSOURI-COLUMBIA
Fiscal Year: 2024
Award: $921,558
Funding agency: National Institute of Allergy and Infectious Diseases
Project Summary
Core 3: Biocontainment Research Support Services Core
The objective of the Biocontainment Research Support Services Core (BRSSC, Core 3) is to provide sustainable
research resources that facilitate the pre-clinical development of medical countermeasures against emerging
and re-emerging pathogens. To achieve this, the BRSSC provides world-class resources in the priority areas of
aerobiology, animal modeling, immunology, imaging, and microbiology for BSL-3 bacterial and viral pathogens.
The BRSSC serves the needs of MU as well as NIAID and the RBL-NBL network by focusing its capabilities to
provide technical expertise under BSL-3 containment for NIAID priority pathogens and the evaluation of novel
vaccines, treatments, and diagnostics. The BRSSC closely collaborates with other relevant Research Services
at MU, including but not limited to the NIH-funded Resource and Research Centers for Mutant Mouse, Rat, and
Swine to develop and refine novel transgenic animal models for priority pathogens such as SARS-CoV-2. The
technical staff of the BRSSC is a dedicated team for conducting and supporting experiments using advanced
technology, high-precision instrumentation on live samples. Added layers of primary and secondary containment
allow for safe handling and usage of the unique support services that are offered in the BRSSC. For example,
direct access from the vivarium to the class III glovebox and its inhalation exposure chamber eliminates the need
to move infected animals through corridors or other space that may expose people. In addition, there is a high-
speed cell sorter, housed within a manufacturer-supplied class II BSC, that allows recovery of live cells infected
with a BSL-3 pathogen for downstream analysis. The Lionheart fluorescent microscopy system allows real-time
imaging of environmentally-controlled live BSL-3 samples in multi-well format, and is useful for optimization of
viral growth conditions and other live and in vitro assays without need for pathogen inactivation. Further imaging
and other instrumentation available in the ABSL-3 allows for longitudinal monitoring of the infection, blood
chemistry and immune responses without need to chemically inactivate sample, thereby reducing time and
unwanted impact for the procedure. All of these examples illustrate the capabilities for safe conduct of BSL-3
research using advanced technology instrumentation that is available through the BRSSC. The collaborative
effort between the dedicated teams of Cores 1, 2 and 3 of this UC7 application combined with regular interactions
with the NIAID RBL-NBL network will maximize best practices and usage of these unique resources. To create
sustainability, the BRSSC is committed to training diverse technical professionals and scientists in BSL-3/ACL-
3/ABSL-3 research. Through management of the critical resources at the LIDR, the BRSSC helps advance the
development of novel medical countermeasures against BSL-3 pathogens.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><Advanced Development><Aerosols><Animal Model><Animal Models and Related Studies><Animals><Area><Assay><BSL-3 facility><BSL3 facility><Bioassay><Biological Assay><Blood Chemical Analyses><Blood Chemical Analysis><COVID crisis><COVID epidemic><COVID pandemic><COVID-19 crisis><COVID-19 epidemic><COVID-19 era><COVID-19 global health crisis><COVID-19 global pandemic><COVID-19 health crisis><COVID-19 pandemic><COVID-19 period><COVID-19 public health crisis><COVID-19 virus><COVID-19 years><COVID19 virus><Caprine Species><Cavia><Cell Body><Cells><Chemicals><CoV-2><CoV2><Collaborations><Common Rat Strains><Communicable Diseases><Communication><Communities><Containment><Controlled Environment><Core Facility><Cricetinae><Custom><Data><Dedications><Development><Development and Research><Diagnostic><Domestic Rabbit><Emerging Communicable Diseases><Emerging Infectious Diseases><Ensure><Equipment><Evaluation><Event><Family suidae><Ferrets><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Microfluorimetry><Flow Microfluorometry><Funding><Generalized Growth><Goals><Goat><Goats Mammals><Growth><Guinea Pigs><Guinea Pigs Mammals><Hamsters><Hamsters Mammals><Human Resources><Image><Immune response><Immunity><Immunological response><Immunology><Infection><Infectious Disease Pathway><Infectious Diseases><Infectious Diseases / Laboratory><Infectious Diseases Research><Infectious Disorder><Inhalation Exposure><Investigators><Investments><Maintenance><Manpower><Manufacturer><Methodology><Methods><Mice><Mice Mammals><Microbiology><Microscopy><Missouri><Modeling><Monitor><Murine><Mus><Mutant Strains Mice><NIAID><NIH><National Institute of Allergy and Infectious Disease><National Institutes of Health><Oryctolagus cuniculus><Ovine><Ovis><Persons><Pigs><Procedures><Productivity><Public Health><R & D><R&D><Rabbits><Rabbits Mammals><Rat><Rats Mammals><Rattus><Recovery><Reproducibility><Research><Research Personnel><Research Resources><Research Support><Researchers><Resource Development><Resources><Route><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV-2 epidemic><SARS-CoV-2 global health crisis><SARS-CoV-2 global pandemic><SARS-CoV-2 pandemic><SARS-CoV2><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-coronavirus-2 epidemic><SARS-coronavirus-2 pandemic><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Safety><Sampling><Scientist><Secure><Services><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome CoV 2 epidemic><Severe Acute Respiratory Syndrome CoV 2 pandemic><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 epidemic><Severe acute respiratory syndrome coronavirus 2 pandemic><Severe acute respiratory syndrome related corona virus 2><Sheep><Specialist><Speed><Structure><Suidae><Swine><System><Technical Expertise><Technology><Time><Tissue Growth><Training><Transgenic Animals><Translating><United States National Institutes of Health><Universities><Viral><Work><Workforce Development><Wuhan coronavirus><artificial environment><bacteria pathogen><bacterial pathogen><biocontainment facility><biocontainment lab><biocontainment laboratory><biodefense><biosafety level 3 facility><blood chemistry><coronavirus disease 2019 crisis><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 pandemic><coronavirus disease 2019 public health crisis><coronavirus disease 2019 virus><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease pandemic><coronavirus disease-19 global pandemic><coronavirus disease-19 pandemic><coronavirus disease-19 virus><customs><cytokine><developmental><emergent outbreak><emerging outbreak><emerging pathogen><experience><experiment><experimental research><experimental study><experiments><flow cytophotometry><hCoV19><host response><human disease><human model><imaging><immune system response><immunoresponse><in vitro Assay><instrumentation><medical countermeasure><model of animal><model of human><mouse mutant><nCoV2><new outbreak><new pathogen><new vaccines><next generation><next generation vaccines><novel><novel outbreak><novel pathogen><novel vaccines><ontogeny><pathogen><pathogen exposure><pathogenic bacteria><pathogenic virus><personnel><porcine><pre-clinical><pre-clinical development><preclinical><preclinical development><priority pathogen><programs><real-time images><realtime image><recruit><research and development><response><severe acute respiratory syndrome coronavirus 2 global health crisis><severe acute respiratory syndrome coronavirus 2 global pandemic><suid><technical skills><vector transmission><viral pathogen><virus pathogen>