Cell biology of SARS-CoV-2 infection

NIH Pandemic-Era Grants

Pandemic Era Grants

2020

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Principal Investigator: Ted  Hackstadt
Organization: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Fiscal Year: 2020
Award: $18,479
Funding agency: National Institute of Allergy and Infectious Diseases

We are using cell culture models to study SARS-CoV-2 replication.  African Green Monkey Kidney (Vero) cells are being used as a model system to study interactions with the host cell.  A time course of viral replication was first evaluated by immunofluorescence and electron microscopy. Using commercially available antibodies to the viral nucleoprotein and spike protein, we detected evidence of nucleocapsid production by 3 hr post-infection and spike protein by 6 hr post infection.  Additional antibodies to the envelope and membrane glycoproteins are in production.  The signals from the spike and nucleoproteins do not overlap.  The nucleoprotein is localized throughout the cytosol, apparently associated with membranous structures that do not bear markers of the endoplasmic reticulum.  These membranes appear to coalesce later during the infection to surround vacuolar-like structure with some internal antigen.  The spike protein is observed in association with multiple cellular membranes including the Golgi apparatus, nuclear envelope, and plasma membrane.  The endoplasmic reticulum shows condensation by 12 hr post-infection.  Efforts to define the localization and cellular effects of individual viral genes are in progress.

Terms: <2019 novel coronavirus><2019-nCoV><African Green Monkey><Antibodies><Antigens><Biologic Models><Biological Models><Cell Body><Cell Communication and Signaling><Cell Culture Techniques><Cell Signaling><Cell Surface Glycoproteins><Cell membrane><Cells><Cellular Membrane><Cellular biology><Chlorocebus aethiops><Chlorocebus sabaeus><Coronaviridae><Coronavirus><Cytoplasmic Membrane><Cytosol><Electron Microscopy><Endoplasmic Reticulum><Ergastoplasm><Golgi><Golgi Apparatus><Golgi Complex><Green Monkey><Immunofluorescence Microscopy><Individual><Infection><Intracellular Communication and Signaling><Kidney><Kidney Urinary System><Membrane><Membrane Glycoproteins><Model System><Modeling><Modification><Nuclear Envelope><Nuclear Membrane><Nucleocapsid><Nucleoproteins><Physical condensation><Plasma Membrane><Production><Proteins><SARS-CoV-2><SARS-CoV2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related coronavirus 2><Severe acute respiratory syndrome coronavirus 2><Signal Transduction><Signal Transduction Systems><Signaling><Structure><Surface Glycoproteins><Time><Vero Cells><Viral><Viral Genes><Virus><Virus Replication><Wuhan coronavirus><biological signal transduction><cell biology><cell culture><condensation><corona virus><immunogen><membrane structure><plasmalemma><renal><viral multiplication><viral replication><virus multiplication>