Structural Biology and Biochemistry of Coronavirus
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Principal Investigator: Audray Harris Organization: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES Fiscal Year: 2020 Award: $37,194 Funding agency: National Institute of Allergy and Infectious Diseases Coronaviruses remain both a seasonal health burden and pandemic threat due to the antigenic variation of viral glycoproteins and the ability of animal coronaviruses to cause zoonotic infections in humans. Although conserved epitopes have been identified, there is a fundamental gap in understanding and correlating the disposition of conserved coronavirus epitopes on subunit vaccines and designed nanoparticles with immunogenicity. Lack of such information represents important problems and until they are addressed optimal display of conserved coronavirus epitopes cannot be understood in molecular details. In FY 2020, we have begun to establish milestones in the application of electron microscopy to study coronavirus structure and biochemistry. This will aid in understanding conserved coronavirus epitope display on viruses and vaccine platforms. This is significant and relevant to public health because it is expected to expand understanding of the structure and epitope disposition of coronavirus epitopes and will aid immunogen evaluation and design for more efficacious coronavirus vaccines. Terms: <2019 novel coronavirus><2019-nCoV><3-D><3-Dimensional><3D><Address><Affect><Animals><Antibodies><Antigen Variation><Antigenic Determinants><Antigenic Variability><Antigenic Variation><Antigens><Architecture><B blood cells><B cell><B cells><B-Cells><B-Lymphocytes><B-cell><Binding><Binding Determinants><Biochemical><Bite><COVID-19 epidemic><COVID-19 pandemic><COVID19 epidemic><COVID19 pandemic><CoV S protein><CoV glycoprotein S><CoV spike glycoprotein><CoV spike protein><Coin><Communicable Diseases><Competence><Complex><Coronaviridae><Coronavirus><Coronavirus glycoprotein S><Coronavirus spike protein><Development><Disease><Disorder><Electron Microscopy><Encapsulated><Engineering / Architecture><Envelope Protein><Epitopes><Evaluation><Formulation><Genome><Geometry><Glycoproteins><Goals><Health><Human><Immune response><Immune system><Immunochemical Immunologic><Immunologic><Immunological><Immunological response><Immunologically><Immunologics><Infectious Disease Pathway><Infectious Diseases><Infectious Disorder><MERS><MERS coronavirus disease><MERS-CoV disease><Membrane><Membrane Protein Gene><Membrane Proteins><Membrane-Associated Proteins><Middle East Respiratory Syndrome><Middle East Respiratory Syndrome CoV disease><Middle East Respiratory Syndrome coronavirus disease><Middle Eastern Respiratory Syndrome><Middle Eastern Respiratory Syndrome CoV disease><Middle Eastern Respiratory Syndrome coronavirus disease><Mission><Modern Man><Molecular><Molecular Interaction><Molecular Modeling Nucleic Acid Biochemistry><Molecular Modeling Protein/Amino Acid Biochemistry><Molecular Models><NIH><Nano platform><Nano-technological platform><Nano-technology platform><Nanoplatform><Nanotechnological platform><National Institutes of Health><Nucleocapsid Proteins><Phase><Proteins><Public Health><Recombinants><Research><SARS Virus><SARS corona virus><SARS coronavirus><SARS-Associated Coronavirus><SARS-CoV><SARS-CoV-2><SARS-CoV2><SARS-Related Coronavirus><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related coronavirus 2><Scaffolding Protein><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe acute respiratory syndrome coronavirus 2><Structural Biochemistry><Structure><Subunit Vaccines><Surface Proteins><T-Cell Epitopes><T-Cells><T-Lymphocyte><T-Lymphocyte Epitopes><Testing><United States National Institutes of Health><Vaccine Design><Vaccines><Viral><Viral Structural Proteins><Viral Vaccines><Virion><Virus><Virus Particle><Wuhan coronavirus><Zoonoses><Zoonotic><Zoonotic Infection><adaptive immune response><allergic/immunologic body system><allergic/immunologic organ system><arm><base><corona virus><corona virus disease 2019 epidemic><corona virus disease 2019 pandemic><coronavirus S protein><coronavirus disease 2019 epidemic><coronavirus disease 2019 pandemic><coronavirus spike glycoprotein><design><designing><develop a vaccine><development of a vaccine><developmental><env Antigens><env Gene Products><env Polyproteins><env Protein><host response><immunogen><immunogenicity><immunoresponse><innovate><innovation><innovative><membrane structure><molecular modeling><nano particle><nano-sized particle><nanoparticle><nanosized particle><nanotechnology platform><neutralizing antibody><pandemic><pandemic disease><particle><scaffold><scaffolding><severe acute respiratory syndrome-CoV><structural biology><structural glycoprotein><structural-GP><three dimensional><thymus derived lymphocyte><vaccine development><vaccine efficacy><vaccine formulation><viral RNA><virus RNA><virus envelope>