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Principal Investigator: George W Jackson
Organization: BASE PAIR BIOTECHNOLOGIES, INC.
Fiscal Year: 2024
Award: $751,525
Funding agency: National Institute of Biomedical Imaging and Bioengineering
Project Summary/Abstract
The United States is experiencing an epidemic of unprecedented scope with heroin use resurging
and a troubling pervasive increase in abuse of prescription opioids such as fentanyl, fentanyl
derivatives, methadone, oxycodone and hydrocodone. More than 6 out of 10 drug overdoses are
now opioid-related, resulting in ~90 deaths/day and a more than $75B/year economic impact.
Additionally, there is a rapidly expanding need to detect numerous other small molecules such as
therapeutic agents, in the home or potential WMDs like sarin and toxic pesticides in the remote
or field setting. To develop a general purpose tool to address such detection, we propose to refine
our compensated interferometric reader (CIR) and further develop a unique and efficient, matrix
insensitive, assay methodology to address the need for high sensitivity, rapid testing of opioids,
therapeutics and environmental contaminants. Our efforts will produce a first of its kind bench-
top (and field-compatible) CIR and user-friendly (turn-key) assay system for the detection and/or
quantification at ng/mL-pg/mL sensitivity of a wide range of targets including opiates, small
molecule drugs, and organophosphorus pesticides. The assay based on a near-surface assay
(NSA) embodiment, consists of three-steps; 1) collect sample (dilute if needed), 2) fill the pre-
coated capillary and 3) read the ‘sample’ with CIR. Results for drug or metabolite detection in
urine by CIR is predicted to be <300 pg/mL sensitivity for the fentanyl family, a >10-1000-fold
improvement over the best lateral flow tests (LFT)1 and the current cutoff for standard drug tests.
The non-invasive, nanoliter volume, high specificity urine or buffer-diluent assays will facilitate
rapid field-based detection of environmental samples, seized illicit narcotics, counterfeit drugs
and organophosphorus nerve agents (OPNA). Similar minimally invasive assays are feasible with
minute amounts of finger-stick blood. The novel methodology is enabled by the marriage of three
patented technologies, a multiplex-SELEX approach for aptamer selection, an aptamer-probe
assay, and CIR to quantify the target. Phase I and Phase II Aims proposed here are designed to
enable rapid translation/commercialization of the proposed technology.
Terms: <Acids><Actinic Rays><Actiq><Adanon><Address><Althose><Assay><Bioassay><Biological Assay><Blood><Blood Reticuloendothelial System><Blood Serum><Blood capillaries><Buffers><Calibration><Cessation of life><Chemicals><Chemistry><Compensation><Computer software><Death><Detection><Development><Dihydrocodeinone><Dihydrohydroxycodeinone><Dolophine><Drugs><Duragesic><Ensure><Environment><Environmental Pollutants><Environmental Toxin><Epidemic><Family><Fentanest><Fentanyl><Fentyl><Fingers><Generations><Gold><Home><Hycodan><Hycon><Hydrocodone><Immobilization><Infumorph><Kadian><LC/MS><Label><Laboratory Research><Laser Electromagnetic><Laser Radiation><Lasers><Legal patent><Letters><Levarterenol><Levonorepinephrine><MS Contin><MSir><Manufacturer><Marketing><Marriage><Medication><Methadone><Methadose><Methodology><Methods><Morphia><Morphine><N-(phosphonomethyl)glycine><Narcotics><Nerve agent VX><Noradrenaline><Norepinephrine><Norfentanyl><Opiates><Opioid><Optics><Oramorph><Oramorph SR><Overdose><Oxycodeinon><Oxycodone><Oxycodone SR><Oxycontin><Patents><Patients><Peer Review><Pesticides><Pharmaceutical Preparations><Phase><Phentanyl><Physicians' Offices><Preparation><Production><Publications><Pump><Reader><Reproducibility><Roxanol><Roxicodone><Rural Hospitals><Sampling><Sarin><Scientific Publication><Serum><Software><Specificity><Statex SR><Surface><Surface Tension><System><Technology><Technology Transfer><Tenofovir><Testing><Therapeutic><Therapeutic Agents><Toxic Environmental Agents><Toxic Environmental Substances><Training><Translations><UV light><UV radiation><UV rays><Ultraviolet Rays><United States><Update><Urine><VX agent><VX gas><VX nerve gas><Vendor><Viread><Walking><Work><aptamer><capillary><commercialization><design><designing><detection platform><detection system><developmental><drug detection><drug sensitivity><drug standard><drug testing><drug/agent><economic impact><environmental contaminant><environmental toxicant><experience><full scale manufacturing><gliphosate><glyphosate><heroin intake><heroin use><homes><improved><instrument><large scale manufacturing><large scale production><lateral flow assay><lateral flow test><liquid chromatography mass spectrometry><mass production><minimally invasive><nano litre><nanoliter><nanolitre><nerve agent><novel><opioid medication abuse><opioid prescription drug abuse><optical><orthopedic freezing><preparations><prescription opiate abuse><prescription opioid abuse><prototype><rapid assay><rapid test><rapid testing><rapid tests><small molecule><tool><translation><ultra violet light><ultra violet radiation><ultra violet rays><ultraviolet light><ultraviolet radiation><user-friendly>