Document text
Principal Investigator: Rong Fan
Organization: YALE UNIVERSITY
Fiscal Year: 2024
Award: $107,220
Funding agency: National Cancer Institute
Summary
The main goals of the Supplement Award are to (1) correlate immunoserological responses to early immune
reactions in local tissue environment of the vaccine injection site in healthy donors, and (2) the compare between
healthy controls and patients with hematological disorders or autoimmune disease. We will use a high plex
immuno-fluorescence CODEX imaging panel to profile these skin biopsy samples in the next year of the award.
Using this panel, we can confirm the expression of the SARS-CoV-2 Spike antigen. Interestingly, the SARS-CoV-
2 Spike antigen was detected in the epidermis and dermis of both arms from two donors. Specifically, SARS-
CoV-2 Spike co-expressed with CD303 and CD63 of the unvaccinated arm tissue of a healthy donor and in
cluster 0 of the vaccinated arm of a BCD donor, indicating expression in dendritic cells and basophils. In the next
year to be supported by the Supplement Award, we will be imaging at least 13 more skin tissue sections and
plan to conduct deeper informatics analysis. Additionally, we are performing DBiT-based spatial CITE-seq on
adjacent slides from these 13 skin tissue sections and integrating the data with previously obtained sc-mRNA-
seq data. Furthermore, we have completed immunoserology assays with the serum samples of these donors
and patients. Therefore, once we complete the peripheral tissue spatial imaging and spatial omics sequencing,
the results will be compared to systemic immunological and serological responses over times to help understand
the role of early immune reaction in the development of an effective vaccine protection, in particular, in patients
with blood cancer or autoimmune disease.
Terms: <2019-nCoV S protein><2019-nCoV spike glycoprotein><2019-nCoV spike protein><2019-nCoV vaccine><Antigens><Assay><Autoimmune Diseases><Autoimmune Status><Autoimmunity><Award><Basophilic Granulocyte><Basophils><Bioassay><Biological Assay><Biopsy Sample><Biopsy Specimen><Blood Basophil><Blood Diseases><Blood Serum><Body Tissues><CITE sequencing><CITE-seq><CITEseq><COVID monitoring><COVID-19 S protein><COVID-19 monitoring><COVID-19 spike><COVID-19 spike glycoprotein><COVID-19 spike protein><COVID-19 vaccination><COVID-19 vaccine><COVID19 monitoring><Cell Body><Cells><Cellular Indexing of Transcriptomes and Epitopes by Sequencing><Communicable Diseases><Corium><Cutis><Data><Dendritic Cells><Dermatologic biopsy><Dermis><Development><Environment><Epidemic><Epidermis><Future><Goals><Hematologic Cancer><Hematologic Diseases><Hematologic Malignancies><Hematologic Neoplasms><Hematological Disease><Hematological Disorder><Hematological Malignancies><Hematological Neoplasms><Hematological Tumor><Hematopoietic Cancer><Hematopoietic Cell Tumor><Hematopoietic Malignancies><Hematopoietic Neoplasms><Hematopoietic Neoplasms including Lymphomas><Hematopoietic Tumor><Hematopoietic and Lymphoid Cell Neoplasm><Hematopoietic and Lymphoid Neoplasms><Image><Immune><Immune reaction><Immunes><Immunity><Immunochemical Immunologic><Immunofluorescence><Immunofluorescence Immunologic><Immunologic><Immunological><Immunologically><Immunologics><Infectious Disease Pathway><Infectious Diseases><Infectious Disorder><Informatics><Injections><Malignant Hematologic Neoplasm><Malignant Hematopoietic Neoplasm><Patients><Peripheral><Role><SARS-CoV-2 S><SARS-CoV-2 S protein><SARS-CoV-2 monitoring><SARS-CoV-2 spike><SARS-CoV-2 spike glycoprotein><SARS-CoV-2 spike protein><SARS-CoV-2 vaccination><SARS-CoV-2 vaccine><SARS-coronavirus-2 vaccine><Sampling><Serology><Serum><Severe Acute Respiratory Syndrome CoV 2 vaccine><Severe acute respiratory syndrome coronavirus 2 S protein><Severe acute respiratory syndrome coronavirus 2 spike glycoprotein><Severe acute respiratory syndrome coronavirus 2 spike protein><Severe acute respiratory syndrome coronavirus 2 vaccination><Severe acute respiratory syndrome coronavirus 2 vaccine><Site><Skin Tissue><Slide><Tissues><Vaccinated><Vaccines><Veiled Cells><arm><autoimmune condition><autoimmune disorder><autoimmunity disease><blood cancer><blood disorder><cancer of blood><cancer of the blood><cellular indexing of transcriptomes and epitopes by single cell sequencing><coronavirus disease 2019 S protein><coronavirus disease 2019 monitoring><coronavirus disease 2019 spike glycoprotein><coronavirus disease 2019 spike protein><coronavirus disease 2019 vaccination><coronavirus disease 2019 vaccine><coronavirus disease monitoring><coronavirus disease-19 vaccine><cutaneous biopsy><cutaneous tissue><data integration><developmental><imaging><immunogen><immunoreaction><improved><insight><mRNA seq><mRNA sequencing><mRNA-seq><mRNAseq><nCoV vaccine><nCoV-19 vaccine><nCoV19 vaccine><non vaccinated><not vaccinated><pandemic><pandemic disease><prevent><preventing><response><severe acute respiratory syndrome coronavirus 2 monitoring><skin biopsy><social role><spike proteins on SARS-CoV-2><unvaccinated><vaccinate against COVID-19><vaccinate against SARS-CoV-2><vaccinate against coronavirus disease 2019><vaccinate against severe acute respiratory syndrome coronavirus 2><vaccination against COVID-19><vaccination against SARS-CoV-2><vaccination against Severe acute respiratory syndrome coronavirus 2><vaccination against coronavirus disease 2019><vaccine against 2019-nCov><vaccine against COVID-19><vaccine against SARS-CoV-2><vaccine against SARS-coronavirus-2><vaccine against Severe Acute Respiratory Syndrome CoV 2><vaccine against Severe acute respiratory syndrome coronavirus 2><vaccine candidates against SARS-CoV-2><vaccine for novel coronavirus><vaccine response><vaccine responsiveness><vaccine-induced response><vaccines preventing COVID><vaccines to prevent COVID>