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Principal Investigator: Virginia Kraus
Organization: DUKE UNIVERSITY
Fiscal Year: 2024
Award: $674,676
Funding agency: National Institute on Aging
PROJECT SUMMARY – Molecular Measures Core (MMC)
The Molecular Measures Core (MMC) provides a centralized resource, analytical tools and expertise to
measure a broad array of molecular analytes in support of Duke OAIC projects in their work to understand and
optimize reserve and resilience. The MMC has served as an integral Resource Core in support of the Duke
OAIC since 2006. For this funding cycle, the MMC will focus on molecular analyses that provide mechanistic
insights into resiliencies at the cellular, tissue or organ levels that are expected to increase our understanding
of the means to optimize reserve and resilience at the whole person level. The MMC will provide molecular
analytic, mentoring and consultative support for a host of Research Education Component (REC) Scholar
Projects, Pilot/Exploratory Studies (PESs) and External Projects (EPs). These projects test hypotheses related
to aging across the lifespan, mechanisms underlying reserve and resilience in human, primates and in vitro,
and involve a range of patient and sample types. The MMC is capable of providing services to measure a
broad array of cellular, molecular, biochemical and metabolic factors. The most pertinent Core services in the
next funding cycle will include molecular measures of inflammation, targeted and untargeted metabolomics,
epigenetic measures of miRNAs related to longevity, molecular measures of biological age, and analyses of
extracellular vesicles. In addition, the MMC will conduct a Developmental Project (MMC-DP) to develop new
capabilities for profiling coronavirus serologies to evaluate prior SARS-CoV2 infection as a modifier of
cardiorespiratory and metabolic reserve in a substudy of the MoTrPAC cohort (Years 1-2). This MMC-DP
validated serological testing will be made available to the Duke OAIC researchers and projects in the latter
years of funding. The MMC will also use the senescent model system (developed in the current funding period)
to test interventions including mimics and inhibitors of ‘longevity miRNA’ and extracellular vesicles to promote
resilience (Years 1-5). In addition, the MMC will provide Pathways Analysis (using Ingenuity Systems) of
molecular data to identify potential biological networks involved in reserve and resilience. The exciting focus on
resilience in this cycle provides an opportunity to explore the latest theories on aging that are particularly
amenable and suited to evaluation by molecular approaches. The application of molecular and pathways
analyses to these projects are expected to provide objective insights into the mechanisms underlying reserve
and resilience in support of the mission of the Duke OAIC.
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