COVID-19: 3D Bioprinted Tissue Models for Respiratory Viruses
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Principal Investigator: Marc Ferrer Organization: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES Fiscal Year: 2024 Award: $85,450 Funding agency: National Center for Advancing Translational Sciences We have established protocols for culturing primary human small airway epithelial cells and alveolar cells in a transwell air-liquid-interface (ALI) model. Tissue biofabrication has been optimized in a 96-well plate format, including the formation of a microvessel network. We are positioning these lung tissue models platforms for testing infectivity of viral variants and anti-viral therapeutics for programs at NCATS, NIH and the broader research and drug development community. We have also developed a vascularized perfused lung epithelial tissue model in a high throughput microfluidics platform to assess of viral infection vasculature leakiness and permeability and thrombotic events seen in COVID-19 patients. We are assessing infectivity of intestine tissues (Mattek) with SARS-CoV2, including studies on long term persistence. We have demonstrated that neural spheroids are infected by brain tropic viruses and shown that infection modulates the neuronal activity of these brain models. We have demonstrated that hepatic cell spheroids are infected by viruses and shown that infection modulates the activity of these liver models. Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><3-D><3-Dimensional><3D><Address><Air><Alveolar><Alveolar Cell><Anti-viral Agents><Anti-viral Therapy><Area><Body Tissues><Brain><Brain Nervous System><COVID associated pneumonia><COVID induced pneumonia><COVID infected patient><COVID patient><COVID pneumonia><COVID positive patient><COVID related pneumonia><COVID-19><COVID-19 associated pneumonia><COVID-19 induced pneumonia><COVID-19 infected patient><COVID-19 infection><COVID-19 patient><COVID-19 pneumonia><COVID-19 positive patient><COVID-19 related pneumonia><COVID-19 viral pneumonia><COVID-19 virus><COVID-19 virus infection><COVID19 infection><COVID19 patient><COVID19 positive patient><COVID19 virus><CV-19><Cell Body><Cells><Clinical><CoV-2><CoV2><Communities><Community Developments><Coronaviridae><Coronavirus><Coronavirus Infectious Disease 2019><Development and Research><Disease><Disorder><Drugs><Encephalon><Epithelial Cells><Epithelium><Event><Hepatic Cells><Hepatic Parenchymal Cell><Hepatocyte><Human><Immune><Immunes><Infection><Influenza Virus><Intestinal><Intestines><Laboratories><Liquid substance><Liver><Liver Cells><Lung><Lung Parenchyma><Lung Respiratory System><Lung Tissue><Medication><Microfluidics><Modeling><Modern Man><NCATS><NIH><National Center for Advancing Translational Sciences><National Institutes of Health><Nerve Cells><Nerve Unit><Neural Cell><Neurocyte><Neurons><Pathology><Perfusion><Permeability><Pharmaceutical Preparations><Physiologic><Physiological><Position><Positioning Attribute><Protocol><Protocols documentation><Public Health><R & D><R&D><Research Activity><Research Support><Respiratory Epithelium><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV-2 associated pneumonia><SARS-CoV-2 induced pneumonia><SARS-CoV-2 infected patient><SARS-CoV-2 infection><SARS-CoV-2 patient><SARS-CoV-2 pneumonia><SARS-CoV-2 positive patient><SARS-CoV-2 related pneumonia><SARS-CoV-2 viral pneumonia><SARS-CoV2><SARS-CoV2 infection><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 infection><Severe acute respiratory syndrome related corona virus 2><Societies><Structure of parenchyma of lung><Structure of respiratory epithelium><System><Testing><Therapeutic Intervention><Tissue Model><Tissues><Tracheobronchial><Translational Research><Translational Science><United States National Institutes of Health><Vaccines><Variant><Variation><Vascularization><Viral><Viral Activity><Viral Diseases><Viral Function><Viral Physiology><Virus><Virus Diseases><Wuhan coronavirus><airway epithelium><anti-viral compound><anti-viral development><anti-viral drug development><anti-viral drugs><anti-viral medication><anti-viral therapeutic><anti-viral therapeutic development><anti-viral therapy development><anti-virals><antiviral development><antiviral drug development><antiviral therapeutic development><antiviral therapy development><atypical pneumonia><beta CoV><beta coronavirus><betaCoV><betacoronavirus><bio-fabrication><bio-printing><biofabrication><bioprinting><bowel><clinical efficacy><corona virus><coronavirus disease 2019><coronavirus disease 2019 associated pneumonia><coronavirus disease 2019 induced pneumonia><coronavirus disease 2019 infected patient><coronavirus disease 2019 infection><coronavirus disease 2019 patient><coronavirus disease 2019 pneumonia><coronavirus disease 2019 positive patient><coronavirus disease 2019 related pneumonia><coronavirus disease 2019 virus><coronavirus disease associated pneumonia><coronavirus disease induced pneumonia><coronavirus disease infected patient><coronavirus disease patient><coronavirus disease pneumonia><coronavirus disease positive patient><coronavirus disease related pneumonia><coronavirus disease-19><coronavirus disease-19 patient><coronavirus disease-19 pneumonia><coronavirus disease-19 virus><coronavirus infectious disease-19><coronavirus patient><developing anti-viral agent><developing anti-viral drug><developing anti-viral therapeutic><developing anti-viral therapy><developing antiviral agent><developing antiviral drug><developing antiviral therapeutic><developing antiviral therapy><drug development><drug repositioning><drug repurposing><drug/agent><fluid><hCoV19><hepatic body system><hepatic organ system><in vitro Assay><infected with COVID-19><infected with COVID19><infected with SARS-CoV-2><infected with SARS-CoV2><infected with coronavirus disease 2019><infected with severe acute respiratory syndrome coronavirus 2><influenzavirus><intervention therapy><liquid><nCoV2><neural><neuronal><novel><pandemic><pandemic disease><parainfluenza virus><patient infected with COVID><patient infected with COVID-19><patient infected with SARS-CoV-2><patient infected with coronavirus disease><patient infected with coronavirus disease 2019><patient infected with severe acute respiratory syndrome coronavirus 2><patient with COVID><patient with COVID-19><patient with COVID19><patient with SARS-CoV-2><patient with coronavirus disease><patient with coronavirus disease 2019><patient with severe acute respiratory distress syndrome coronavirus 2><pneumonia due to COVID><pneumonia due to COVID-19><pneumonia due to SARS-CoV-2><pneumonia due to coronavirus disease><pneumonia due to coronavirus disease 2019><pneumonia due to severe acute respiratory syndrome coronavirus 2><pneumonia in COVID><pneumonia in COVID-19><pneumonia in SARS-CoV-2><pneumonia in coronavirus disease><pneumonia in coronavirus disease 2019><pneumonia in severe acute respiratory syndrome coronavirus 2><programs><pulmonary><repurposing agent><repurposing medication><research and development><respiratory><respiratory tract epithelium><respiratory virus><response><severe acute respiratory syndrome coronavirus 2 associated pneumonia><severe acute respiratory syndrome coronavirus 2 induced pneumonia><severe acute respiratory syndrome coronavirus 2 infected patient><severe acute respiratory syndrome coronavirus 2 patient><severe acute respiratory syndrome coronavirus 2 pneumonia><severe acute respiratory syndrome coronavirus 2 positive patient><severe acute respiratory syndrome coronavirus 2 related pneumonia><spheroids><three dimensional><thrombotic><translation research><translational investigation><viral infection><viral infectious disease treatment><virus infection><virus-induced disease><µfluidic><β CoV><β coronavirus><βCoV>