Structural Biology Core
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Principal Investigator: Wah Chiu Organization: STANFORD UNIVERSITY Fiscal Year: 2022 Award: $3,880,029 Funding agency: National Institute of Allergy and Infectious Diseases ABSTRACT An integrated Structural Biology Core will be established to enable the Research Projects’ scientists employing state-of-the-art tools for structural determinations from RNA, protein to virus and virus infected cells. This Core is made up of three Subcores including protein production, x-ray crystallography and scattering, and cryogenic electron microscopy and tomography. All the imaging and diffraction instruments are located at the SLAC National Accelerator Laboratory campus of Stanford University while the protein production is embedded in the ChEM-H Institute on the main campus. All of the facilities are maintained by experienced staff at SLAC under the supervision of Drs. Chiu, Hedman, and Hodgson. The Core will be staffed by specialists to assist and train the AViDD Center’s scientists in performing the structure characterizations to enable the design of novel and effective antivirals against SARS-CoV-2 and other RNA viruses of pandemic potential. Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><3-D visualization><3-dimensional visualization><3D visualization><Antiviral Agents><Antiviral Drugs><Antivirals><Binding Proteins><Biochemical><COVID-19 virus><COVID19 virus><Cell Body><Cells><CoV-2><CoV2><Complex><Computer software><Cryo-electron Microscopy><Cryoelectron Microscopy><Crystallization><Crystallographies><Crystallography><DNA Molecular Biology><Data><Data Analyses><Data Analysis><Data Collection><Development><Devices><Drug Targeting><Drug Therapy><Electron Cryomicroscopy><Electron Microscope><Electron Microscopy><Electron Microscopy Facility><Faculty><Fluorescence><Funding><High Throughput Assay><Image><Informatics><Infrastructure><Institutes><Intracellular Structure><Investigators><Ions><Joints><Knowledge><Laboratories><Ligand Binding Protein><Ligand Binding Protein Gene><Macromolecular Structure><Membrane><Membrane Protein Gene><Membrane Proteins><Membrane-Associated Proteins><Methodology><Methods><Microscopy><Modeling><Molecular Biology><Molecular Structure><NIGMS><National Institute of General Medical Sciences><Non-Polyadenylated RNA><Out-patients><Outpatients><Pathologic><Pharmacotherapy><Preparation><Production><Protein Binding><Proteins><R-Series Research Projects><R01 Mechanism><R01 Program><RNA><RNA Gene Products><RNA Viruses><Research Grants><Research Personnel><Research Project Grants><Research Projects><Research Resources><Researchers><Resolution><Resources><Ribonucleic Acid><Robotics><Roentgen Rays><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV2><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Scientist><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Single Crystal Diffraction><Software><Specialist><Structure><Subcellular structure><Supervision><Surface Proteins><Synchrotrons><System><Temperature><Time><Training><Universities><Validation><Viral><Viral Gene Products><Viral Gene Proteins><Viral Proteins><Virus><Wuhan coronavirus><X Ray Crystallographies><X-Radiation><X-Ray Crystallography><X-Ray Diffraction Crystallography><X-Ray Radiation><X-Ray/Neutron Crystallography><X-ray><Xray><Xray Crystallography><anti-viral agents><anti-viral compound><anti-viral drugs><anti-viral medication><anti-viral therapeutic><anti-virals><antiviral compound><antiviral medication><antiviral therapeutic><base><bound protein><cell imaging><cellular imaging><computerized data processing><coronavirus disease 2019 virus><coronavirus disease-19 virus><cryo-EM><cryoEM><cryogenics><data interpretation><data management><data processing><data reduction><design><designing><developmental><drug treatment><electron tomography><experience><hCoV19><high throughput screening><image processing><imaging><instrument><instrumentation><macromolecule><membrane structure><nCoV2><nano meter><nanometer><novel><pandemic><pandemic disease><particle><protein expression><rational design><reconstruction><screening><structural biology><structural genomics><synchrotron radiation><three-dimensional visualization><tomography><tool><viral RNA><virus RNA><virus protein>