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Principal Investigator: Andrew Zelhof
Organization: TRUSTEES OF INDIANA UNIVERSITY
Fiscal Year: 2024
Award: $106,561
Funding agency: NIH Office of the Director
Project Summary:
The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists
utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide
broad access to genomics resources by acquiring, authenticating, archiving, curating, and distributing
genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics
resources by providing guidance and support; and 3) improve the genomics resources and protocols
available for Drosophila research. By preserving vital research materials and distributing them efficiently,
the DGRC assures economical access to important research reagents, and enhances scientific rigor and
reproducibility. The first aim of this proposal is to continue to strengthen the successful DGRC programs
by: A) Continue to acquire, archive, curate, and distribute Drosophila resources including clones,
vectors, and cell lines for supporting fundamental research of biological processes and modeling of
aspects of human disease. B) Pilot a program with Indiana University Clinical and Translational
Science Institute (CTSI) to promote Drosophila genomics reagents for use in non-Drosophila labs;
C) Manage the cost recovery program and resource center to maximize the long-term viability of the
DGRC and the benefits to its users and the NIH. The second aim is to improve and update our
curation process, data management systems and web interface, and incorporate FAIR and TRUST
guiding principles into our reagents, metadata and linking data to other repositories. The goal is to
maximize the long-term viability of the DGRC and its benefits to users and the NIH. The third aim is to
increase the utility of Drosophila as a model system by generating new resources and protocols through
four projects: A) Increase the authentication, and reproducibility and quality control of DGRC reagents; B)
Generate gut derived cell lines and pilot and expand to generate a neuroblast derived cell line; C)
Continue to expand the catalog of recombination mediated cassette exchange compatible stable attP
Drosophila cell lines; D) Collaborate to develop metabolic sensors and standards for aerobic glycolysis
and other biosynthetic pathways.
Terms: <Anterior><Applied Research><Applied Science><Archives><Autoregulation><Behavior><Biologic Models><Biological Function><Biological Models><Biological Process><Biomedical Research><COVID crisis><COVID epidemic><COVID pandemic><COVID-19 crisis><COVID-19 epidemic><COVID-19 era><COVID-19 global health crisis><COVID-19 global pandemic><COVID-19 health crisis><COVID-19 pandemic><COVID-19 period><COVID-19 public health crisis><COVID-19 years><CRISPR approach><CRISPR based approach><CRISPR method><CRISPR methodology><CRISPR technique><CRISPR technology><CRISPR tools><CRISPR-CAS-9><CRISPR-based method><CRISPR-based technique><CRISPR-based technology><CRISPR-based tool><CRISPR/CAS approach><CRISPR/Cas method><CRISPR/Cas technology><CRISPR/Cas9><CRISPR/Cas9 technology><Cancers><Cas nuclease technology><Catalogs><Cell Body><Cell Communication and Signaling><Cell Cycle><Cell Division Cycle><Cell Line><Cell Signaling><CellLine><Cells><Cessation of life><Chromatin Assembly><Chromatin Modeling><Clinical Sciences><Clustered Regularly Interspaced Short Palindromic Repeats approach><Clustered Regularly Interspaced Short Palindromic Repeats method><Clustered Regularly Interspaced Short Palindromic Repeats methodology><Clustered Regularly Interspaced Short Palindromic Repeats technique><Clustered Regularly Interspaced Short Palindromic Repeats technology><Collaborations><Communities><Complementary DNA><Coupled><Data><Data Management Resources><Data Management System><Death><Development><Disease><Disorder><Drosophila><Drosophila genome><Drosophila genus><Drosophila melanogaster><Epigenetic><Epigenetic Change><Epigenetic Mechanism><Epigenetic Process><Erinaceidae><Evolution><Funding Agency><Funding Source><Gene Action Regulation><Gene Expression Regulation><Gene Regulation><Gene Regulation Process><GeneHomolog><Genes><Genetic><Genome><Genomics><Goals><Grant><Hedgehogs><Homeostasis><Homolog><Homologous Gene><Homologue><Human><Indiana><Individual><International><Intracellular Communication and Signaling><Link><M Phase><Malignant Neoplasms><Malignant Tumor><Meiosis><Metabolic><Metadata><Mission><Mitosis><Mitosis Stage><Model System><Modeling><Modern Man><NIH><National Institutes of Health><Nerve Degeneration><Neurobiology><Neuron Degeneration><PTK Receptors><Pathway interactions><Pattern><Physiological Homeostasis><Plasmid Cloning Vector><Plasmid Vector><Population><Post-Transcriptional Gene Silencing><Posttranscriptional Gene Silencing><Process><Protocol><Protocols documentation><Quality Control><RNA Interference><RNA Silencing><RNAi><Reagent><Receptor Protein-Tyrosine Kinases><Receptor Tyrosine Kinase Gene><Recovery><Reproducibility><Research><Research Resources><Resources><SARS-CoV-2 epidemic><SARS-CoV-2 global health crisis><SARS-CoV-2 global pandemic><SARS-CoV-2 pandemic><SARS-coronavirus-2 epidemic><SARS-coronavirus-2 pandemic><Scientist><Sequence-Specific Posttranscriptional Gene Silencing><Severe Acute Respiratory Syndrome CoV 2 epidemic><Severe Acute Respiratory Syndrome CoV 2 pandemic><Severe acute respiratory syndrome coronavirus 2 epidemic><Severe acute respiratory syndrome coronavirus 2 pandemic><Signal Pathway><Signal Transduction><Signal Transduction Systems><Signaling><Specific qualifier value><Specified><Strains Cell Lines><Synapses><Synaptic><Technology><Translational Research><Translational Science><Transmembrane Receptor Protein Tyrosine Kinase><Tyrosine Kinase Linked Receptors><Tyrosine Kinase Receptors><United States National Institutes of Health><Universities><Update><Work><aerobic glycolysis><biological signal transduction><cDNA><cDNA Library><catalog><coronavirus disease 2019 crisis><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 pandemic><coronavirus disease 2019 public health crisis><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease pandemic><coronavirus disease-19 global pandemic><coronavirus disease-19 pandemic><cost><cultured cell line><depository><developmental><epigenetically><fruit fly><fruit fly genome><fundamental research><gene conservation><gene replacement><genome resource><genomic data resource><genomic resource><genomic sequencing resource><human disease><improved><malignancy><meiotic><meta data><neoplasm/cancer><neural><neural degeneration><neurobiological><neuroblast><neurodegeneration><neurodegenerative><neurological degeneration><neuronal degeneration><notch><notch protein><notch receptors><pathway><preservation><programs><recombinase-mediated cassette exchange><recombination-mediated cassette exchange><repository><sensor><severe acute respiratory syndrome coronavirus 2 global health crisis><severe acute respiratory syndrome coronavirus 2 global pandemic><success><synapse><translation research><translational investigation><vector><web interface>