Document text
Principal Investigator: Jason G Kay
Organization: STATE UNIVERSITY OF NEW YORK AT BUFFALO
Fiscal Year: 2024
Award: $381,667
Funding agency: National Institute of Dental and Craniofacial Research
Project Summary/Abstract
Oral commensal microbes, including oral streptococci, are increasingly recognized as an essential
component in the development of periodontal disease. Commensals contribute to the recruitment and growth of
keystone disease-initiating microbes and are part of the dysbiotic disease-associated microbiome.
Innate immune phagocytes, including macrophages, also play an essential role in the development of
periodontal disease, and phagocytes themselves are phenotypically diverse. Interactions between phagocytes
and keystone pathogens are well studied. However, relatively little is understood of the interactions between
phagocytes and commensal streptococci. Also unknown is how an initial immune disruption by keystone
pathogens can alter host immune interactions with oral streptococci that may enhance the latter’s contribution to
disease.
We have data highlighting a novel, yet counterintuitive, relationship between macrophages and S. gordonii:
the bacterium is better able survive within, and promote enhanced cytokine release from, inflammatory
macrophages. This new data, along with the knowledge that active periodontitis lesions have an increase in
inflammatory macrophages leads us to our central hypothesis that upon initiation of inflammation, changes in
macrophage-oral streptococci interactions allow the normally commensal bacterium to increase resistance to
destruction and leads to enhanced cytokine production and inflammation. We will test our hypothesis by 1)
assessing the molecular details of phagosomal escape and subsequent inflammatory promotion of S. gordonii
within phagocytes, 2) determine the mechanisms of S. gordonii inflammatory modulation of macrophages initially
activated by the keystone pathogen P. gingivalis and 3) begin to examine the in vivo mechanisms of increased
S. gordonii survival within inflammatory macrophages.
The overall aim of this research is to increase our understanding of the consequences of changes in host-
commensal interactions upon initiation of inflammation. The rationale is that with an increased understanding of
the conditions and molecular mechanisms by which commensals can act as pathobionts, we will achieve a first
step in the long-term goal of developing new targets and strategies for the prevention and treatment of oral
bacterial-associated inflammatory diseases.
Terms: <Active Oxygen><Acute><Bacteria><Bacteroides gingivalis><Blood leukocyte><Body Tissues><Buccal Cavity><Buccal Cavity Head and Neck><Cavitas Oris><Cell Body><Cell Death><Cells><Chronic Periodontitis><Cytoplasm><Data><Development><Disease><Disorder><Endocarditis><Environment><Exposure to><Generalized Growth><Goals><Growth><Immune><Immune Cell Activation><Immune system><Immunes><Inflammasome><Inflammation><Inflammatory><Knowledge><Lesion><Leukocytes><Leukocytes Reticuloendothelial System><Macrophage><Marrow leukocyte><Membrane><Microbe><Molecular><Mouth><Mφ><Oral><Oral cavity><Oxygen Radicals><P gingivalis><P. gingivalis><Parodontosis><Pathway interactions><Periodontal Diseases><Periodontitis><Phagocytes><Phagocytic Cell><Phagosomes><Phenotype><Play><Porphyromonas gingivalis><Preventative strategy><Prevention strategy><Preventive strategy><Pro-Oxidants><Production><Publishing><Reactive Oxygen Species><Receptor Protein><Research><Resistance><Rodent><Rodentia><Rodents Mammals><Role><S gordonii><S. gordonii><Streptococcus><Streptococcus gordonii><Testing><Tissue Growth><Tissues><Vesicle><White Blood Cells><White Cell><amebocyte><commensal bacteria><commensal bacterial species><commensal flora><commensal microbes><commensal microbiota><commensal microflora><cytokine><developmental><dysbacteriosis><dysbiosis><dysbiotic><host microbe association><host microbe relationship><host-microbe interactions><host-microbial interactions><host-microorganism interactions><immune activation><in vivo><in vivo Model><inflammatory modulation><membrane structure><microbial imbalance><microbiome><microorganism><necrocytosis><novel><ontogeny><oral commensal><oral streptococci><pathobiont><pathogen><pathway><periodontal disorder><periodontium disease><periodontium disorder><prevent><preventing><receptor><recruit><resistant><response><social role><tissue repair><white blood cell><white blood corpuscle>