Document text
Principal Investigator: Julie Biteen
Organization: UNIVERSITY OF MICHIGAN AT ANN ARBOR
Fiscal Year: 2024
Award: $307,257
Funding agency: National Institute of General Medical Sciences
Project Summary
Molecular-scale interactions enable subcellular organization, but the current state-of-the-art does not include a
generalizable method for measuring how molecules move and cooperate on the nanometer scale and in real
time within the cell. This gap is particularly striking in bacteria cells. Accordingly, our picture of the organization
of the bacterial cell is incomplete. This proposal aims to understand how cellular components organize—by
scaffolding, aggregation, phase separation, or otherwise—to produce a general model of bacterial cell
organization and ultimately enable us to promote commensal bacteria or fight human disease. Thus, we aim to
measure the positioning, interactions, and motions of molecules in living bacterial cells in different protein
systems implicated in the sub-cellular organization of these cells. This proposal will develop these next-
generation super-resolution tools through three synergistic specific aims: (1) to super-resolve how single-
molecule dynamics vary in space and in time in living bacterial cells; (2) to super-resolve sub-cellular interactions
in space and time in living bacteria cells; and (3) to improve the detection of single molecules in living bacteria
cells. The toolkit that we develop to map molecular motions and interactions will be broadly applicable to the
single-molecule bacteriology community. Furthermore, by focusing on applications in bacterial cell biology, the
tools developed in this project will have a widespread, positive biomedical impact by making accessible long-
term, significant questions in microbial biology.
Terms: <Address><Bacteria><Bacterial Model><Bacteriology><Biochemical Reaction><Biochemistry><Biological Chemistry><Biology><Biophysics><Blue-Green Algae><Blue-Green Bacteria><C crescentus><C. crescentus><Caulobacter crescentus><Cell Body><Cell Function><Cell Physiology><Cell Process><Cells><Cellular Function><Cellular Physiology><Cellular Process><Cellular biology><Color><Communities><Cyanobacterium><Cyanophyceae><Cyanophyta><DNA><Data Set><Deoxyribonucleic Acid><Detection><Diffusion><Disease><Disorder><E coli><E. coli><Enzymatic Reaction><Escherichia coli><Eukaryota><Eukaryote><Fluorescence><Goals><Health><Human><Image><Imaging Device><Imaging Instrument><Imaging Tool><In Vitro><Maps><Measurement><Measures><Methods><Modeling><Modern Man><Molecular><Motion><Organization Charts><Outcome><Phase><Physical condensation><Position><Positioning Attribute><Prokaryotae><Prokaryotic Cells><Proteins><Resolution><Ribonucleoproteins><Role><Structure><Subcellular Process><System><Testing><Time><Tube><Work><biophysical foundation><biophysical principles><biophysical sciences><cell biology><cell fixing><cell type><commensal bacteria><commensal bacterial species><condensation><data quality><diffused><diffuses><diffusing><diffusions><fighting><human disease><imaging><improved><in vitro Model><innovate><innovation><innovative><microbial><molecular scale><nano><nano meter scale><nano meter sized><nanometer scale><nanometer sized><nanoscale><new approaches><next generation><novel><novel approaches><novel strategies><novel strategy><organizational structure><prokaryote><resolutions><scaffold><scaffolding><single molecule><social role><spatiotemporal><statistics><super high resolution><superresolution><superresolution imaging><superresolution microscopy><tool><ultra high resolution>