Development of Nipah virus mRNA vaccine

NIH Pandemic-Era Grants

Pandemic Era Grants

2023

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Principal Investigator: Rosemarie  Mason
Organization: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Fiscal Year: 2023
Award: $929,036
Funding agency: National Institute of Allergy and Infectious Diseases

For this study we developed and optimized a robust, high-throughput (384-well format) ELISA binding assay to quantify Nipah virus (NiV) pre-fusion (pre-F)- and glycoprotein (G)-specific serum antibody binding responses. We also developed and optimized a highly reproducible NiV lentivirus vector-based pseudo-typed neutralization assay for both 96-well and 384-well formats. Serum samples from rhesus macaques immunized with Nipah virus pre-F or G proteins were used to optimize and validate these assays. These NiV ELISA binding and neutralization assays were then used to assess NiV mRNA-1215 vaccine-induced serum antibody responses for binding to pre-F and monomeric G proteins, and for neutralization of NiV pseudo-typed virus in vaccinated African green monkeys (AGM) (study VRC-22-0989) and humans (study VRC322).

Terms: <African Green Monkey><Antibodies><Antibody Response><Assay><Binding><Bioassay><Biologic Assays><Biological Assay><Blood Serum><Cessation of life><Chimera Protein><Chimeric Proteins><Chlorocebus aethiops><Chlorocebus sabaeus><Coma><Comatose><Death><Development><Disease><Disorder><ELISA><Enzyme-Linked Immunosorbent Assay><Fusion Protein><G-Proteins><GTP-Binding Proteins><GTP-Regulatory Proteins><Glycoproteins><Green Monkey><Guanine Nucleotide Coupling Protein><Guanine Nucleotide Regulatory Proteins><Human><Immunize><Lentiviral Vector><Lentivirus Vector><Licensing><Link><M mulatta><M. mulatta><Macaca mulatta><Messenger RNA><Modern Man><Molecular Interaction><Monomeric G-Proteins><Monomeric GTP-Binding Proteins><Nipah Virus><RNA vaccine><RNA-based vaccine><Reproducibility><Rhesus Macaque><Rhesus Monkey><Sampling><Serum><Small G-Proteins><Small GTPases><Vaccinated><Vaccines><Viral Diseases><Virus><Virus Diseases><developmental><enzyme linked immunoassay><mRNA><mRNA vaccine><mRNA-based vaccine><monomer><response><vaccine candidate><viral infection><virus infection><virus-induced disease>