Development of a high precision and high order DNA counting platform

NIH Pandemic-Era Grants

Pandemic Era Grants

2024

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Principal Investigator: Eleen Yee Lam Shum
Organization: ENUMERIX, INC.
Fiscal Year: 2024
Award: $824,307
Funding agency: National Institute of General Medical Sciences

PROJECT SUMMARY/ABSTRACT
Enumerix has developed a cost-effective method to perform high order DNA counting at high precision
called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of
partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and
thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from
the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital
PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation
sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase
II project we plan to further develop our dcPCR platform with the goal to distribute the technology to
end-users to replace quantitative PCR and digital PCR.

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