Optimizing in vivo dLight recordings for investigating the influence of disease on dopamine signaling.
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Principal Investigator: Jacob Roshgadol Organization: UNIVERSITY OF CALIFORNIA AT DAVIS Fiscal Year: 2024 Award: $41,972 Funding agency: National Institute of Mental Health Project Summary This proposed research aims to provide a roadmap for studying dopamine dynamics using a novel optical tool, dLight, deepening our understanding of dopamine biology. Altered dopamine signaling is central to many neurological and neuropsychiatric disorders, yet we lack effective treatments for these disorders. Many existing treatments act on dopaminergic systems, but they act on slow time scales and do not work for all patients. Deepening our fundamental understanding of dopamine biology can lead to the discovery of improved treatments. The Tian lab developed dLight, which is an optical biosensor that can directly record dopamine signaling in vivo with high precision. The proposed work aims to determine the performance metrics of the newest dLight variants in vivo and use the sensor to measure alterations in dopamine activity in a disease model. Specifically, I will measure differences in dopamine signaling between offspring of the maternal immune activation model and healthy controls, as offspring from this model have been shown to have altered dopamine signaling systems. Additionally, I will create a database of the data collected to share with the neuroscience research community, increasing the accessibility of dLight and enabling researchers to use this novel tool in their own research. Successful completion of this project has the potential to transform our understanding of dopamine biology and significantly contribute to the development of effective treatments for many neurological and neuropsychiatric disorders. Terms: <2-photon microscopy><Acceleration><Address><Affinity><Ammon Horn><Amygdala><Amygdaloid Body><Amygdaloid Nucleus><Amygdaloid structure><Animal Disease Models><Animals><Atlases><Behavior><Benchmarking><Best Practice Analysis><Biology><Biosensor><Brain><Brain Diseases><Brain Disorders><Brain Nervous System><Brain region><Cell Communication and Signaling><Cell Signaling><Characteristics><Communities><Complex thinking><Cornu Ammonis><Corpus Striatum><Corpus striatum structure><Critical Thinking><DA Neuron><Data><Data Analyses><Data Analysis><Data Bases><Databases><Development><Disease><Disorder><Dopamine><Dopamine neuron><Dysfunction><Encephalon><Encephalon Diseases><Engineering><Evaluative Thinking><Event><Family><Functional disorder><Generations><Grooming><Hippocampus><Hydroxytyramine><Image><Immune Cell Activation><Immunology><In Vitro><Inflammatory><Intracellular Communication and Signaling><Intracranial CNS Disorders><Intracranial Central Nervous System Disorders><Investigators><Knowledge><Literature><Location><Measurement><Measures><Metadata><Methods><Modeling><Nature><Nervous System Diseases><Nervous System Disorder><Neurologic Disorders><Neurological Disorders><Neuromodulator><Neurosciences><Neurosciences Research><Optics><Outcome><Paralysis Agitans><Parkinson><Parkinson Disease><Pathology><Patients><Performance><Phenotype><Physiologic><Physiological><Physiopathology><Play><Prefrontal Cortex><Primary Parkinsonism><Research><Research Personnel><Researchers><Rewards><Role><Schizophrenia><Schizophrenic Disorders><Shapes><Signal Transduction><Signal Transduction Systems><Signaling><Striate Body><Striatum><System><Technology><Testing><Time><Training><Translating><Variant><Variation><Visualization><Work><amygdaloid nuclear complex><behavior phenotype><behavioral phenotyping><benchmark><biological sensor><biological signal transduction><calcium indicator><cognitive process><conditioned fear><data base><data interpretation><dementia praecox><design><designing><developmental><disease model><disorder model><dopamine system><dopaminergic neuron><effective therapy><effective treatment><efficacious therapy><efficacious treatment><fear conditioning><hippocampal><imaging><immune activation><improved><in utero><in vivo><insight><interest><internet resource><meta data><multiplexed imaging><neural><neural control><neural regulation><neurological disease><neuromodulation><neuromodulatory><neurophysiological><neurophysiology><neuropsychiatric disease><neuropsychiatric disorder><neuroregulation><new approaches><new technology><novel><novel approaches><novel strategies><novel strategy><novel technologies><offspring><on-line compendium><on-line resource><online compendium><online resource><open source><open source tool><open source toolkit><optical><optogenetics><pathophysiology><repetitive behavior><response><schizophrenic><sensor><skills><social role><striatal><technological innovation><theories><tool><two photon excitation microscopy><two photon microscopy><web resource><web-based resource>