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Principal Investigator: Kathryn Ann Wikenheiser-Brokamp
Organization: CINCINNATI CHILDRENS HOSP MED CTR
Fiscal Year: 2024
Award: $135,832
Funding agency: National Institute of Diabetes and Digestive and Kidney Diseases
PROJECT SUMMARY
The overall goal of the Integrative Morphology Core of the Digestive Health Center (DHC) is to provide
DHC investigators with state-of-the-art, high quality, efficient and reliable technology support for morphologic
studies of digestive diseases. The Core pursues this goal with three complementary aims. In the First Aim “to
develop an effective service infrastructure to support morphologic studies,” the Core developed and
implemented an organizational infrastructure and personnel to greatly facilitate the request and execution of
services in a cost-efficient basis, while enhancing interactions between the investigative groups. The Core is
directed by an experienced pathologist with expertise on molecular biology and advanced instrumentation for
high-definition analysis of fixed and living cells and tissues. In the Second Aim “to augment microscopic
analyses of tissue and cellular structure and function,” the Core staffs three laboratories with highly qualified
technicians or directors in three complementary laboratories: 1) Research Pathology Laboratory, focusing on
histochemical, immunostaining, and electron microscopy analysis of cells and tissues; 2) Live Microscopy
Laboratory that specializes in 2-photon-based confocal microscopy of live cells and tissues; and 3) Confocal
Imaging Laboratory, with a superb infrastructure for high-end confocal microscopy. In the Third Aim “to
ensure quality control, cost efficiency, and technology transfer,” the Core implements a quality assurance
program related to reliability of reagents, well-functioning instruments, user safety, proper hands-on-training,
and streamlined process for service requests. The Core has evolved with the acquisition of new
instrumentation, incorporation of artificial intelligence to deconvolute digital information and increase
the resolution of confocal microscopy, and collaboration with the Gene Analysis Core and the Biobank
to perform spatial sequencing. The Core also holds seminars and workshops as part of the Center
enrichment series with the goal to provide DHC investigators with practical reviews and analysis of the latest
technologies and assays, and to empower investigators to take advantage of the resources based on their
scientific needs. This management strategy and commitment to research excellence have resulted in large
utilization of services and have been linked to a high scientific output in peer-reviewed original
publications that are relevant to digestive disease.
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Instrumentation><Confocal Microscopy><Consultations><Cost efficiency><DNA Molecular Biology><Danio rerio><Data><Decontamination><Digestive Diseases><Digestive System Diseases><Digestive System Disorders><Digestive Tract><Disease><Disorder><Doctor of Philosophy><Educational workshop><Electron Microscopy><Electrons><Ensure><Evolution><Experimental Designs><Fluorescence><Foundations><GI Tract><GI tract disorder><Gastrointestinal Tract><Gastrointestinal tract structure><Genes><Goals><Health><Hour><Human><Human Resources><Image><Image Analyses><Image Analysis><Immune><Immunes><Infrastructure><Injury><Institution><Intracellular Communication and Signaling><Investigators><Investments><Laboratories><Leadership><Link><Liver><Machine Intelligence><Manpower><Medical center><Medicine><Methods><Mice><Mice Mammals><Microscope><Microscopic><Microscopy><Model System><Modern Man><Molecular Biology><Morphology><Murine><Mus><Names><Negative Beta Particle><Negatrons><Nucleic 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method><Stains><Subcellular Process><Surface><Technology><Technology Transfer><Tissues><Training><Translational Research><Translational Science><Video Recording><Videorecording><Visualization><Workshop><Zebra Danio><Zebra Fish><Zebrafish><alimentary tract><base><bases><bench bed side><bench bedside><bench to bed side><bench to bedside><bench to clinic><bench to clinical practice><biobank><biological signal transduction><biorepository><college><collegiate><confocal imaging><consultation><coronavirus disease 2019 crisis><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 pandemic><coronavirus disease 2019 public health crisis><coronavirus disease 2019 testing><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease pandemic><coronavirus disease testing><coronavirus disease-19 global pandemic><coronavirus disease-19 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coronavirus 2 global pandemic><severe acute respiratory syndrome coronavirus 2 testing><super high resolution><superresolution><testing for COVID><testing of COVID><three dimensional><translation research><translational investigation><two-photon><ultra high resolution><video recording system><web based software><web software>