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BNT162b2
Module 2.6.1. Introduction
CONFIDENTIAL
Page 1MODULE 2.6.1. INTRODUCTION
This document contains confidential information belonging to BioNTech /Pfizer. Except as may be 
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information in confidence and not to disclose it to others (except w here required by applicable 
law), nor to use it for unauthorized purpos es. In the event of actual or suspected breach of this 
obligation, BioNTech /Pfizer should be promptly notified.
090177e19630e2b2\Approved\Approved On: 08-Feb-2021 15:28 (GMT)
FDA-CBER-2021-5683-0708841
BNT162b2
Module 2.6.1. Introduction
CONFIDENTIAL
Page 22.6.1. INTRODUCTION
There is an urgent need for the development of a new proph ylactic vaccine given the threat 
posed by  the increasing number of globall y distributed outbreaks of Severe Acute 
Respiratory  Syndrome (SARS) -CoV -2 infection and thus its associated disease Coronavi rus 
Disease 2019 (COVID -19). A L ipid Nanoparticle (LNP) -formulated ribonucleic acid (RNA )-
based vaccine would provide one of the most flexible, scalable, and fastest approaches to 
provide protection against new, fast spreading, virus infection. The develop ment of a n
RNA -based vaccine encoding a viral antigen that is translated by  the vaccinated organism to 
protein to induce a protective immune response provides significant advantages over more 
conventional vaccine approaches.
BNT162b2 (BioNTech code number BNT162, Pfizer code number PF -07302048) is a
vaccine intended toprevent COVID -19, which is caused bySARS -CoV -2.BNT162b2, 
otherwise known as BNT162b2 (V9), is a nucleoside modified mRNA (modRNA) 
expressing full -length spike (S) protein with two proline mutations (P2) to lock the 
transmembrane protein in an antigenicall y optimal prefusion conformation . The vaccine is 
formulated in lipid nanoparticles. The LNP is composed of 4 lipids: AL C-0315, AL C-0159, 
DSPC, and cholesterol. Other excip ients in the formulation include sucrose, NaCl, KCL, 
Na2HPO 4, and KH 2PO 4. The drug product is a preservative -free, sterile dispersion of RNA 
formulated in LNP in aqueous cry oprotectant buffer for intramuscular ( IM)administration. 
The RNA drug substance is the only  active ingredient in the drug product. The drug product 
is a concentrate for injection and filled a mg/mL  in glass vials and closed with 
stoppers and flip off crimping cap.
2.6.1.1. Proposed Indications
BNT162b2 is indicated for a ctive immuniz ation to prevent COVID -19caused by  SARS -
CoV -2 virus, in individuals 16years of age and older .The dose selected for BNT162b2 for 
commercial use i s 30 ug RNA administered IM on Day s1 and 22.
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