110 1 Courtesy Copy BLA 125742 0 Analytical Method Review Memo COMIRNATY

Pfizer Documents (PHMPT/FDA)

Pfizer Bla Submission

Pfizer 16 Plus Documents

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DEPARTMENT OF HEALTH & HUMAN SERVICES   
Food & Drug Administrat ion 
Silver Spring, MD 20993 
 1 
 
 
DBSQC/OCBQ ANALYTICAL METHOD REVIEW MEMO 
 To: The file: STN 125742/0  
 
From :  
 
Reviewer  Date 
finalized  Stamp  Supervisor  Stamp  
Emnet Yitbarek  
(Lead reviewer)  8/20/2021     Kori Francis    
Hsiaoling Wang  8/19/2021     Tao Pan    
Esmeralda 
Alvarado-
Facundo  8/20/2021     Muhammad Shahabuddin      
Anil Choudhary  8/20/2021     
Karla Garcia  8/20/2021    James Kenney    
Simleen Kaur  
  
 Through: Maryna Eichelberger, Ph.D., Division Director, CBER/OCBQ/DBSQC  
 
Mary A. Malarkey, Director CBER/OCBQ  
Applicant : BioNTech  Manufacturing GmbH  
 Subject:  Review of Analytical Methods used for the Lot release of COMI RNATY 
Drug Substance (DS) and Drug Product  (DP) 
____________________________________________________________________  
 Recommendation:  Approval  
 Summary:  
The following analytical methods used for lot release of COMIRNATY and the 
associated method validation s or qualifications, were  reviewed:  
1. RNA  DP by  (Emnet Yitbarek)  
2. Identifi cation and quantification of lipids in DP by  (Emnet Yitbarek ) 
3.  (Hsiaoling Wang)  
4.  (Hsiaoling 
Wang)  
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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5. Gener al  methods  to test  DP: Appearanc e, Particu late 
Matter ,  (Hsiaoling Wang)  
6. Identi ty of  DP by   Assay ( Esmeralda Alvarado- Facundo)  
7.  (Esmeralda Alvara do-Facundo)  
8.  (Esmerald a Alvarado -Facundo) 
9.  (Anil Choudhary ) 
10.  (Anil Choudhary ) 
11.  (Anil Chou dhary ). 
12.  (Karla Garc ia) 
13. Endotoxin of   DP (Karla Garc ia) 
14. Sterility of DP (Karla Garcia)  
 
Conclusion:  The analytical methods and their validations and/or qualifications 
reviewed for the COMIRNATY drug substance and drug product  were found to be 
adequate for their inte nded use.  
 
Documents Reviewed  
Information in sections of the original BLA (STN125742) and IND 19736 submissions 
that descr ibe control of DS  and DP (3.2.S.4 and 3.2.P.5, respecti vely) , including 
descriptions of DS  and DP s pecifica tions,  analytical procedures of DS an d DP and 
validation of these analytical procedures were reviewed. Additional information in amendmen ts specified by each reviewer  were also reviewed.  
Background On Ma y 6, 2021, Pfizer/BioNTe ch (sp onsor)  submitte d an original r olling BLA, STN 
125742, for COMIR NATY (BNT162/PF -07302048), a prophylactic vaccine for the 
prevention of COVID -19 which is  caused by SARS- CoV-2 virus. COMIRNATY is a 
white suspension solution composed of mRNA t hat encodes for the spike protein of 
the SARS-C oV-2 virus and is encapsulated i n a lipid nano particle  (LNP). The 
concentrated suspension is stored at - 60 °C and is diluted with a sterile 0.9% Sodium 
Chloride solution , USP,  to ad minister  intramuscularly to individuals ≥16 years  of age. 
The proposed dosage is a 30 µg regimen of two 0.3 mL doses  given 3 weeks apart.  
 DBSQC reviews BLAs and their supplements to ensure analytical methods are appropriately described, validated and suitable for the intended purposes.  The 
following facilities  perfor m the  methods review ed: 
1. Pfizer Biotherapeutics Pharmaceutical Sciences  Analytical Rese arch & 
Development , Chesterfield, MO ( ARD- STL)  
2. Pfizer Biotherapeutics Pharmaceutical Sciences  Analytical Rese arch & 
Development ,
  Andover,  MA (ARD- AND) 
3. Pfizer Global Supply, Kalamazoo, MI  (PGS -KZO)  
4. Pfizer Global Supply , Andover , MA ( PGS- AND) 
5. Pfizer Global Supply, Puurs, Belgium  (PGS -Puurs)  
6. Pfizer Global Suppl y, Grange Castle, Ire land ( PGS- GC) 
7.   
 
 2 
(b) (4)
(b) (4)
(b) (4)
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 3  The following analytical methods used for DS and DP release were reviewed: 
1. RNA  DP by   
Method description 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
  
  
 
 
 
 
  
 
  
 
  
 
  
 
 
 
 
 
 
 
(b) (4)
(b) (4)
(b) (4)
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 6  
 
 
 
 
 
 
 
 
 
 
 
 
 
Conclusion  
The  method is adequately validated for the determination of product  in  
COMIRNATY DP . Th e firm’s test  method i ncludes limited instructio ns for 
. They have agreed to add the information and submit  the updated 
procedure to their IND.  
 
2. Identification and quantification of lipids in DP  by   
Method descri ption  
The identi ty and quantit y of the four lipids in the DP , ALC-0 159, c holesterol, DSPC 
and ALC- 0315, are determined by . In this method,  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 7 The assay system su itability criteria  include:  
  
  
  
Assay and DP sample  acce ptance criteria include:  
  
 
  
  
 
  
 
 
  
Method Validation: 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
(b) (4)
(b) (4)
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 9  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Conc lusion :  
The  method is adequately described and validated for the 
identification and quantitat ion of four lipids (ALC- 0159, cholesterol, DSPC and ALC -
0315) in COMIRNATY DP. 
 
 
 
 
  
(b) (4)
(b) (4)
(b) (4)
(b) (4)
3 pages have been determined to be not releasable
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 13  
 
 
 
  
 
 
 
 
 
  
 
 
4.  of DP by   
The DP is a sterile  of RNA -containi ng lipid nanoparticles (LNPs) in 
phosphate buffer contai ning sucrose. The specifications of LNP  for DP are 
, respectively. The specification for 
the . 
   
Method description  
  
  
 
 
  
 
 
 
 
  
 
   
 
(b) (4)
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 17  
 
 
Conclusion  
Based on the information provided in the or iginal BLA and t he amendment, the 
method has been validated for its intended purpose.  
 
 
5. General  Methods to test  DP  
 methods used for  DP analyses are shown in T able 7 below.  
 Table 7.  methods used to tes t  DP 
 
 These methods do not require validation. Suitability of the methods for this  DP was verified as described below.  
 
Review of Appearance Methods and Method Verifications : 
The appearanc e of  includes the tests for . The appearance 
tests described in TM100010539 for ARD  labs and in LAB -  for PGS -AND lab are 
equivalent.  
 
 
 
  
DP Test Specification   
method  
DP  Appearance: Visual 
and particles  White to off -white suspension; May 
contain white to off-white opaque, 
amorphous particles  
DP   
 
DP   
DP      
DP Container content  Not less than  
(b) (4)
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21 Review of Container Content and Method Verifications : 
The container content is an adaption of the  for DP. Analytical 
procedures in TM100011129 for ARD labs, LAB-  for PGS -Puurs and TM9106A 
for PGS -KZO are equivalent.  DP vials are used and 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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The contai
ner content is calculated to  decimal places and every one of the  vials 
should have a volume of no less than  to be reported as “No Less Than 
”. 
Infor
mation Request and Reviews : 
The following IR was sent  to the firm on August 2, 2021:  
a.You calculate volume of each vial based on . Please 
describe how  was determined.  
b.In the verification report [
] from PG S-KZO lab, DP container content was 
determined by measuring the total volume after 1.8 mL of sterile 0.9% sodium 
chloride solution was added. Please confirm that this method will be used for lot release testing by the PG S-KZO laboratory and that the container volume 
specification “Not less than ” is the same regardless of test site/method.  
Review of the response: The response was received on August 9, 2021 in amendment 35.  
a.The firm stated that the 
  was obtained by  
b.The firm confirmed that the container con tent specification of Not less than
  is the same regardless of test site/method. Container content for 
  was used as a DP specification under EUA, which was replaced by 
22 
(b) (4)
(b) 
(4)
(b) (4)
(b) 
(4)
(b) (4)
(b) (4)
(b) (4)
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vial container content in BLA. PGS -KZO will perform DP container cont ent test 
as other labs described in analytical procedure TM9106A.  
 
The responses are acceptable. Re -verification from PGS -KZO is not necessary 
because the analytical steps are equivalent.    
 23 Conclusion  
Based on the information provided in the original BLA  and the amendment, these  
 methods have been verified for their intended purposes.  
 
6. Identi ty of  DP by  Assay 
The  assay  is a release test to verify the  identity of  DP by 
demonstrating the presence of the mRNA target. The test is  performed at Pfizer 
facilities  PGS -GC, PGS -AND, A RD a nd . The spec ificatio n for  DP is  
“Identity confir med”.  
Method description 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 24 The assay validity criteria are:  
 
 
 
 
 
 
 
If the assay fails to meet  predefined acceptance criteria, then the assay is invalid  and 
must be repeated. If the DP assay acceptance crit eria fail, the sample is re -
processed f rom th e beginning . 
The sample acceptance criteria are:  
 
 
 
 
 
 
If the assay acceptance criteria and sample acceptance criteria are met,  the result is 
reported as “ Identity C onfirme d” for the presence of target mRNA  or “Identity not 
confirmed” for the absence of target mRNA.  
Method Va lidatio n 
 
 
 
 
 
 
 
 
 
  
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 25 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Conclusion:  
The  method to confirm the identity of   DP mRNA was adequately 
descri bed and the validation data demons trate the assay is suitabl e for its intended 
use. 
 
 
 
 
 
  
 
(b) (4)
(b) (4)
(b) (4)
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 36   
 
  
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
10. Total  RNA by   
 
The principle of the method t o quantify RNA  is  
 Method De
scription   
  
 
 
 
 
 
 
 
 
 
 
 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 37  
 
 
 
 
 
 
  
 
  
 
 
 
 
A
ssay acceptanc e criteria are as f ollows : 
 
 
 
 
 
 Sample accepta nce criteria are as follow: 
 
 
 
  
 
 Reporting of the Results : The total RNA content  is reported for each test sample in 
; the  RNA is reported as a whole 
number.  The current specifications for DP  are:  
.  
Method Qualification  
  
 
 
 
 
 
(b) (4)
(b) (4)
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 39  
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
Overall Conclusion  
The RNA  method is adequately described and demonstrated to be 
suitable for measuring total RNA and .  
 
 
 
 
 
 
 
(b) (4)
(b) (4)
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(b) (4)
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 45  13 . Bacter ial Endotoxin Test  (BET)  
Pfizer  qualified the  BET m ethod at the ARD -AND a nd PGS-
AND facilities  for testing of  BNT162b2 mRNA . In addition, t he  
 method was also qualified at the PGS -AND facility. The -BET 
method was performed usin g a  and -BET method was performed 
using the  
 the BET for Drug Product  (DP), which also uses  a 
. The BNT162 b2 DP 
is a single -stranded, 5 ’-capped mRNA that is translated into a protein. Lipids are later 
added to form  lipid nanopartic les (LNP);  
 the DP BET is perfor med.    
 
Method des c ription  
 
 
 
 
 
 
-BET  Me thod Qualif ication  for  
  
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
(b) (4)
(b) (4)
(b) (4)
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 46  
 
 
 
 
 
 
 
 
 
 
 
 
 
  
  
  
BET Qualification for D rug Produc t  
Pfizer  qualified the -BET method for DP  at the Pfizer Global Supply  in Puurs, 
Belgiu m (PGS- Puurs ) and Pfizer Global Supply in  Kalamazoo, MI ( PGS- KZO) 
facilities . 
 
-BET Method Qualification for BNT162b2 mRNA DP: 
The -BET is performed to dete ct or quantitate bacterial endoto xins present  in test 
samples .  
 
 
 
 
 
  
 The original submiss ion d id not include qualification data from the PGS -Puurs and 
PGS- KZO laboratories. CBER sent an i nformation request  (IR) to Pfizer  on July16, 
2021, regar ding their t est for interfering factors at  the PGS -Puurs and P GS-KZO 
facilities.  Respons e to this IR w as received on July  30, 2021 in amendment num ber 
21.   Pfizer  submitted  supplemental verification  report s performed on DP at each 
requested facility . These verification tests were performed with  
 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 47  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
The c urrent DP bacterial endotoxin test method involves sample preparation in which 
 
 Pfizer  performed a bacterial endotoxin  test to 
demonstrate the  
. This   test is described below:  
 
 
 
 
 
(b) (4)
(b) (4)
(b) (4)
(b) (4)
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 49  
  
 
 
  
 
  
 
 
 
 
 
 
 
Conc lusion   
The endotoxin ( ) test meth od was qualified 
appropriately  and demonstrated to be suita ble unde r the actual c ondit ions of use . 
Pfizer  has committed  to  
and wi ll sub mit the verification study  result s 
with a proposed updated lot release protocol template a s a supplement to this file on 
or before December 6 , 2021.  
 
 14.  Sterility T est  
Method descripti on 
The  is used t est DP sterility.  
 
 
 
 
 
 
 
 
 
 
 
 
    
 
 
 
 
 
(b) (4)
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 52  
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
Conclusion :  The  Sterility Test method was validated in 
accordance with  and was found t o provi de detec tion results equival ent 
 sterility test method.  The data provided 
show that  the  method is suitable for its intended use.   
 
(b) (4)
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