Combined archive search across FBI Vault, SPLC, PHMPT, ACIP, NIH grants, the Federal Writers' Project, and other collections we've ingested.
ROBERT A SEDER · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2023 · $332,304 — Over the past year we have focused on how mucosal vaccination influences immunity and protection against Omicron variants of concerned compared to conventional mRNA boosting. For these studies, NHP are immunized with mRNA 1273 as the standard primary vaccine and then boosted several months later wit…
T. Michael Redmond · NATIONAL EYE INSTITUTE · 2023 · $404,162 — Novel coronavirus SARS-CoV-2, originating at the end of 2019 in Wuhan, China, causes the pandemic coronavirus disease COVID-19 that has had a major public health and economic impact in the USA and the rest of the world since then. The disease is quite heterogeneous and targets internal organs with m…
KATHERINE D MCREYNOLDS · CALIFORNIA STATE UNIVERSITY SACRAMENTO · 2023 · $106,500 — PROJECT SUMMARY/ABSTRACT The current COVID-19 pandemic has resulted in millions of infections and over 2 million deaths worldwide since SARS-CoV-2 emerged in Wuhan, China in December of 2019. This pandemic has revealed a significant treatment gap in the ability to minimize the morbidity and mortali…
Haichao Wang · FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH · 2023 · $418,750 — PROJECT SUMMARY Microbial infections annually claim hundreds of thousands of victims in the U.S. alone, but its complex pathogenesis is still poorly understood. Our seminal discovery of HMGB1 as a late mediator of lethal endotoxemia (Science, 1999, Cited >4,000 times) has fueled continuous search fo…
Veronica Villanueva · RUSH UNIVERSITY MEDICAL CENTER · 2023 · $22,422 — Lupus nephritis (LN) and coronavirus disease 2019 (COVID-19) both display myeloid cell dysfunction which can lead to altered signaling resulting in tissue damage, such as kidney injury. Up to 50% of LN patients develop chronic kidney disease (CKD) and 25% of COVID-19 patients are reported to experie…
Maria Antoninova NINOVA · UNIVERSITY OF CALIFORNIA RIVERSIDE · 2023 · $388,750 — Project Summary Transposable elements (TEs) are mobile genetic elements that can propagate within the host DNA and have a significant impact on the genome organization and function. TEs are ubiquitous across the tree of life and occupy substantial fractions of animal genomes - about 20% of the D. m…
Leonid B. Margolis · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · 2023 · $1,895,506 — 1. Cytokines in HIV disease We followed longitudinally 90 people living with HIV (PWH) (59 women and 31 men) already virally suppressed and on antiretroviral therapy (ART) on average for 6 years. We measured the concentrations of 41 cytokines in their blood plasma and followed their trajectory over…
Michael Grigg · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2023 · $454,297 — Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), the etiologic agent of COVID-19, emerged in 2019 as a public health emergency and global pandemic. SARS-CoV-2 is a linear, non-segmented, capped, polyadenylated positive-sense, single stranded RNA virus related to SARS-CoV and MERS-CoV, t…
Dax A Hoffman · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · 2023 · $2,031,130 — Kv4.2 complex regulation and its role in cognitive flexibility We have recently identified a novel molecular cascade that regulates the potassium channel Kv4.2 association with the auxiliary subunit DPP6 and membrane surface expression neurons. This cascade is initiated by various patterns of acti…
Heather Kalish · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · 2023 · $789,255 — Sub-project #1 MALDI imaging of tissue samples to identify novel proteins MALDI-TOF Imaging is an emerging tool for the label free measurement of peptides, proteins, lipids, drugs and metabolites. The tissue can be imaged directly be the MALDI-TOF and combined with other modalities to study the mo…
Mario Borgnia · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2023 · $487,097 — We have established high-throughput structure determination workflows using single particle analysis cryo-EM (SPA) on variants of the SARS-CoV-2 S protein ectodomain. These workflows allow us to solve the structure of macromolecular complexes of the spike at near atomic resolution with less than 24 …
Robert Petrovich · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2023 · $1,405,912 — The protein expression facility (PEF) functions as a support group for the principal investigators at the NIEHS. Our focus is to provide the research groups with a means to generate the proteins they require so that they can perform their experiments. These projects range from generating enough o…
JERREL L YAKEL · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2023 · $1,741,729 — The Viral Vector Core facility currently offers support for production, purification, titering, and validation of twelve types of viruses: Adenovirus, Adenoassociated virus (AAV), coronaviruses (229E, OC43, NL63, and MHV), Herpes Simplex Virus (HSV), Influenza A, lentiviruses including human immunod…
Mitchell Ho · DIVISION OF BASIC SCIENCES - NCI · 2023 · $290,927 — During the pandemic, Dr Ho has evaluated the challenges for developing antibody therapeutics targeting SARS-Cov-2 and wrote a review/perspective article [Ho Antibody Therapeutics 2020; PMID: 32566896]. SARS-CoV-2 gains entry to human cells through its spike (S) protein binding to angiotensin-convert…
alexander wlodawer · DIVISION OF BASIC SCIENCES - NCI · 2023 · $217,282 — Recently, angiotensin-converting enzyme 2 (ACE2) was shown to be a functional receptor for SARS-CoV-2, enabling the virus to enter host target cells. Using structure-based rational design, a chimeric ACE2 peptide was designed by us for use as a ligand for analytical quantitation of SARS-CoV-2. We ai…
di s xia · DIVISION OF BASIC SCIENCES - NCI · 2023 · $149,621 — My group studies membrane proteins using both X-ray crystallography and cryo-EM. For the proposed work, we have obtained/purified recombinant ectodomain of the S protein, the S1 subunit, the RBD domain, the S2 subunit, and the human ACE-2 with the amount sufficient for cryo-EM studies. Currently, we…
David Margulies · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2023 · $66,875 — We have successfully engineered and produced a number of SARS-CoV-2 proteins, subunits, and single domain antibodies directed against the RBD. These purified proteins have provided the materials for obtaining high resolution crystal structures of four of these sybodies in complex with the Spike rece…
Raphaela Goldbach-Mansky · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2023 · $10,739 — In collaboration with the NIAID COVID consortium we have analyzed longitudinally collected whole blood samples collected for RNA isolation from 84 adult patients hospitalized with severe COVID. We also assessed serum samples from a larger panel of adult patients hospitalized with severe COVID (from …
Florian Krammer · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2023 · $612,607 — The SARS-CoV-2 pandemic represents an exceptional public health crisis highlighting the need for better understanding of the mechanisms controlling broadly protective immune responses and generating vaccine candidates able to elicit such responses. The program project entitled “Programming Long-last…
Yihong Ye · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · 2023 · $779,674 — Proteinaceous inclusions termed Lewy bodies (LBs) are a classical hallmark of Parkinson's disease (PD). The primary component of these inclusions is alpha-synuclein (a-syn), a protein with an intrinsic propensity to misfold and aggregate. In PD patients, alpha-syn inclusions first observed in the ol…